Optical Microscopes

  • Inverted confocal microscope
  • Multiphoton excitation and non-descanned detection with FLIM option
  • Internal spectral detection unit

Multi-functional fluorescence inverted confocal microscope with single- or multi-photon excitation, internal spectral detection and non-descanned detection with FLIM option.

Applications

  • Fast and multi-color confocal imaging
  • Various measurement options: z-stack, time series, tile scan, multi positions and regions
  • Spectral detection, spectral unmixing and fingerprinting
  • Deep imaging of thick biological samples with multiphoton excitation and non-descanned detection
  • Label free imaging: Coherent anti-Stokes Raman Scattering (CARS) and higher harmonic generation (SHG and THG)
  • FLIM with multiphoton excitation
  • Long term live-cell imaging available

Specifications

Motorized inverted fluorescence microscope Carl Zeiss Axio Observer.Z1 with confocal module LSM 880 NLO equipped with the following:

Software
ZEN 2.1 Black with following modules and functionalities:
Spectral Unmixing, 3D visualization, Tiles & Positions, FRAP, Experiment designer
Illumination
  • Transmitted Light Illuminator HAL 100
  • Epifluorescence Illuminator HXP 120 V
  • Lasers (CW)
    • 405 nm Laser 30 mW
    • Argon Laser (458, 488, 514 nm) 25 mW
    • 561 nm Laser 20 mW
    • 633 nm Laser 5 mW
  • Lasers (pulsed)
    • Ti:Sapphire femtosecond laser Chameleon Ultra II (Coherent),
      690-1080 nm, > 3.5W
    • Optical parametric oscillator (Chameleon Compact OPO, Coherent),
      1100-1300 nm
    • Delay line with LMS-270 precision liner stage (PI) in the Chameleon
      beam path
Detection
  • 2x transmitted light detector LSM T-PMT
  • Internal spectral detection unit
    • 2 standard PMTs („blue“ and „red“ 371-740 nm)
    • Spectral detector 32-channels GaAsP PMT (410-695 nm)
    • Spectral GaAsP detector and „red“ PMT enables photon counting mode
  • Non-descanned detection unit (reflected geometry)
    • Spectral detector 32BiG.2 unit (containing 2 detectors)
    • High Speed Hybrid Detector for TCSPC HPM-100-40 (Becker & Hickl), IRF 120 ps
    • Range of dichroic beam-splitting filter cubes
Objectives
Objective TypeImmersionMagnificationN.A.WD[mm]DIC/Correct. Ring
EC Plan-Neofluar
420330-9901
Air5x0.1618.5-
Plan-Apochromat
420640-9900
Air10x0.452.10-
LD LCI Plan-Apochromat
420852-9871
Oil, Water, Glycerine25x0.80.57*DIC, Corr (CG=0-0.17mm)
C-Apochromat
421867-9970[1]
Water40x1.10.62*DIC, Corr (CG=0.14-0.19mm)
C-Apochromat
421787-9970[1]
Water63x1.20.28*DIC, Corr (CG=0.14-0.19mm)
Plan-Apochromat
420782-9900[1]
Oil63x1.40.19DIC
* at CG = 0.17
Filter Cubes (turret with 6 positions)
Filter set 49 DAPI EX 365, BS 395, EM 445/50exchangeable
Filter set 38 GFPEX 470/40, BS 495, EM 525/50exchangeable
Filter set 43 Cy3EX 545/25, BS 570, EM 605/70exchangeable
BS-MP 355/690+(R)fixed position
BS-MP-760fixed position
Rear (100% mirror)fixed position
On request (instead of one of the sets above): Filter set 50 Cy5: EX 640/30, BS 660, EM 690/50
Beam splitters in scanning head
Main beam splitters 1 (visible)Main beam splitters 2 (invisible)
MBS 458MBS 405
MBS 458/514MBS 445
MOBS 458/561MBS 690+
MBS 488MBS 760+
MBS 488/561MBS 405/760+
MBS 488/561/633MBS 445/760+
MBS T80/R20MBS T80/R20
PlatePlate
NoneNone
Confocal scanning module LSM 880
13 fps for 512x512, zoom 0.6 - 40x, max. resolution 8192 x 8192
FLIM Option
BiG.2 and TCSPC HPM-100-40 non-descanned detectors can be on request attached to a TCSPC card (TimeHarp 260 Nano, PicoQuant)
  • Spinning disk confocal microscope
  • Dual camera, 7 laser wavelengths
  • FRAP/Photoactivation module
  • Heating/cooling stage top incubator

Versatile spinning disk confocal microscope equipped with seven excitation lasers, epifluorescence LED light source, and two back-illuminated sCMOS cameras. The microscope is equipped by stage-top heating/cooling incubator for live cell and tissue imaging and a FRAP/photoactivation module. It is based on an inverted widefield microscope Nikon Eclipse Ti2 equipped with motorized XY stage, Perfect Focus System, semi-motorized DIC, LED transmitted light source, multicolor LED for wide-field epifluorescence, piezo stage for fast z-stack acquisition, and automatic water dispenser for long term imaging.

Applications

  • Fast multicolor confocal imaging
  • Various measurement options: z-stack, time series, tile scan, multi positions
  • Brightfield and DIC microscopy
  • FRET imaging

Specifications

Software
NIS-Elements
Spinning disk module
Yokogawa CSU-W1
Pinhole size [μm]
(dual disk)
25, 50
Laser excitation wavelengths [nm]
(all CW)
405, 445, 488, 515, 561, 594, 640
Dichroic mirrors (spinning disk module)
quad-band dichroic mirror 405/488/561/638 nm
tripple-band dichroic mirror 445/514/594 nm
Image splitting mirrors (spinning disk module)
561 nm long-pass dichroic mirror (DM A561LP)
514 nm long-pass dichroic mirror (DM A514LP)
empty position for single camera acquisition
Filter cubes (microscope)
DAPI/FITC/TRITC/Cy5
CFP/YFP/mCherry
FITC
TRITC
Emission filters
455/50, 480/40m, 525/50, 545/40, 600/50, 645/75, 690/50, 720/60, 455/50, 480/40, 525/50, 545/40, 440/521/607/700 nm quad-band bandpass filter,
475/543/702 nm triple-band bandpass filter,
Dual Line Emitter 405 / 488
Objectives
Objective NameMagnitudeImmersionN.A.WD[MM]
CFI Plan Apo Lambda 10X10air0.454
CFI Plan Apo VC 20x20air0.751
CFI Plan Apo Lambda S 25XC Sil25silicone oil1.050.55
CF Plan Apo VC 60XC WI60water1.200.31-0.28
CFI SR HP Plan Apo Lambda S 100XC Sil100silicone oil1.350.31-0.28
CFI Plan Apo VC 100X Oil100oil1.400.13
Epifluorescence source
CoolLED pE-4000 (365, 385, 405, 435, 460, 470, 490, 500, 525, 550, 580, 595, 635, 660, 740, and 770 nm)
Tube lenses
1x, 1.5x
Relay lens magnification
1x, 2x, motorized changer
Camera (2x)
PRIME BSI (Teledyne Photometrics)

2048 x 2048 pixels
pixel size: 6.5 x 6.5 μm
63 fps @ 2048 x 2048 pixels, 11-bit data
QEmax> 95%

  • Inverted confocal microscope
  • WLL and internal spectral detection unit
  • FLIM and FCS

Versatile inverted fluorescence confocal microscope equipped with 405 nm, 445 nm and white light laser, spectrally tunable detection and FLIM hardware. Presence of camera and epifluorescence lamp allows also widefield imaging. For more detailed microscope characteristics please see the section “Specifications”.

Applications

  • Multi-color confocal imaging
  • Multi-color widefield imaging
  • Various measurement options: z-stack, time series, tile scan, multi positions
  • Spectral imaging
  • Measurement of emission and excitation spectra 
  • Lifetime imaging, FLIM-FRET
  • Measurement of e.g. membrane dynamics by FCS and FRAP
  • Live-cell imaging available: stage-top incubator with controlled humidity and atmosphere (CO2+N2
  • Brightfield, DIC, polarized light microscopy

Specifications

An inverted confocal microscope Dmi8 with a laser scanning confocal head Leica TCS SP8, a motorized microscope stage with a Super Z-galvo scanning insert for fast and precise Z movement, HW autofocus and Best focus control, transmitted light LED U-12V, square pinhole of tunable size and following units:

Software
LAS-X
Epifluorescence
CoolLED pE-300
Lasers
  • 405 nm(max 40 MHz, 3 mW)
  • 445 nm(max 40 MHz)
  • WLL 470-670 nm (max 80 MHz, 1.5 mW)
    • laser wavelength and intensity is controlled by a combination of AOTF and AOBS
    • the actual wavelength selectable with 1 nm step
    • up to 8 parallel laser lines
Additional beamsplitters
BP 440/40
Filter turret (filter cubes for epifluorescence lamp)
DAPI EX 325-375, BS 400, EM 435-485
FITCEX 460-500, BS 505, EM 512-542
RHODEX 541-551, BS 560, EM 565-605
CY5EX 590-650, BS 660, EM 662-738
Analyser
Objectives
Objective TypeMagnificationImmersionN.A.WD[mm]DIC/Correct. Ring
HC PL FLUOTAR 1.6x1.6xDry0.051.54-
HC PL APO CS 10x10xDry0.402.20-
HC PL APO CS2 20x20xDry0.750.62DIC
HC PL APO CS2 63x WI63xWater1.200.30DIC, motorized correction collar
HC PL APO CS2 63x Oil63xOil1.400.14DIC

Scanner
FOV SP8 scanner
  • maximal sampling 8192x8192px
  • speed 1-1800 Hz (7fps@512x512 px)
  • hardware zoom 0.75x-48x
Fluorifier Disc Settings (notch filters)
Turret 1Turret 2
NF 488NF 445
NF 514NF 594
NF 458/514NF 445/594
NF 488/561/633SMD1 NF 405/470
MBS 488/561SMD2 NF 405/640
MBS 488/561/633SMD3 NF 470/640

Detection
Transmitted light detector (PMT)
Internal spectrally tunable detection unit
  • 3x HyD detectors for FLIM (time-gating possible), GaAsP, 400 – 720 nm, modes: Standard, Counting, BrightR; QE=45%@500nm
  • 2x PMT detectors,400-800nm, QE=30%@500nm

Camera DFC365 FX (Leica)
  • 1392 x 1040 pixels
  • 6.45 μm x 6.45 μm
  • CCD sensor Sony ICX285
  • C-mount 0.7x
FLIM and FCS module
HydraHarp400 (Picoquant)
  • Picosecond Event Timer & TCSPC Module
  • Four channels available
  • Enables detection of photon arrival times with respect to the beginning of the experiment, the excitation pulse and the channel in which the photon was registered (TTTR mode). In combination with a scanner controller, a histogram acquisition at different image positions is available.
Symphotime64
  • Software for image acquisition and analysis
Custom written software in LabView for image acquisition and data analysis.
Navigator module
LAS-X Navigator - software module for quick navigation in the sample and automated stage experiments
Live cell imaging
OKOlab incubation system - regulation of temperature and CO2 and N2 level
  • Inverted confocal microscope
  • STED super-resolution microscopy

Fluorescence inverted confocal microscope and STED super-resolution nanoscope with high detection efficiency of far-red photons. The system is equipped with four excitation lasers, depletion laser 775 nm and four single photon counting detectors. For more detailed microscope characteristics please see the section “Microscope”. Basic introduction to STED microscopy can be found here:

https://abberior-instruments.com/knowledge/microscopy-tutorials/sted/

Applications

  • Two-color 2D and 3D super-resolution images obtained by STED technique with a pulsed depletion laser 775 nm and pulsed excitation 561 nm and 640 nm 
  • STED RESCUE and DYMIN mode available (STED imaging mode that significantly reduces the light dose sent onto the sample without compromising the resolution)
  • 2D and 3D STED images available also with water immersion objective
  • Multicolor confocal scanning system with variable pinhole size
  • Possible FLIM or FCS acquisition
  • Live cell imaging
  • NIR confocal imaging (775 nm excitation)

Specifications

Inverted confocal microscope Nikon Eclipse Ti-E equipped with a piezo Z-stage, motorized XY stage, Perfect Focus System, transmitted light lamp (100 W) and following units:

Software
Imspector (v16.3)
Epifluorescence
CoolLED pE-4000
Epifluorescence cubes
FITC (465-495, DM505, 515-555)
TRITC (527-553, DM565, 577-633)
QUAD (DAPI/FITC/Cy3/Cy5)
Analyzer
Excitation lasers
cw - 405 nm, pulsed - 485 nm, 561 nm, 640 nm, and 775 nm
STED Laser
775 nm (40 MHz pulsed laser, for 2D and 3D STED)
Donut formation
Programmable Spatial Light Modulator – 775 nm
Scanner
Abberior QUAD scanner: line frequency up to 2.5 kHz
Objectives
Nikon CFI Plan Apo Lambda 60x Oil, NA 1.40, WD 0.13 mm
Nikon CFI Plan Apo 60x WI, NA 1.27, WD 0.17 mm, Correction Collar 0.15-0.19, DIC
Nikon CFI Plan Apo Lambda 10x, NA 0.45, WD 4 mm, DIC
Emission filters
422-467 nm
506-594 nm (or 500-550 nm)
580-630 nm
650-720 nm (or 800-870 nm)
Pinholes
16 pinholes from 25 μm to 2 mm
Detectors
4x Photon Counting Module (Excelitas Technologies)
  • Two time resolved detectors optimized for FLIM
  • High detection sensitivity for red light (>62% for 680nm)
PMT for transmitted and reflected light (Hamamatsu Photonics)
Monochrome widefield camera DMK 23U274 (Imaging Source)
  • CCD sensor, 1600×1200 (1.9 MP), up to 20 fps, pixel size 4.4 µm x 4.4 µm
FLIM
Externally connected TCSPC card: TimeHarp260N (PicoQuant)
  • Two independent channels, ultra-short dead time <2ns, temporal resolution 250 ps
  • Inverted widefield microscope
  • TIRF illumination
  • Image splitter

Multifunctional fluorescence inverted widefield microscope enabling live-cell imaging, TIRF or HILO illumination and two super-resolution techniques: structured illumination microscopy and single molecule localization microscopy. For more detailed microscope characteristics please see the section “Specification”. Basic introduction to super-resolution microscopy can be found here:

http://zeiss-campus.magnet.fsu.edu/articles/superresolution/introduction.html

Applications

  • Multicolor (up to 3 colors) super-resolution images obtained by 3D-SIM technique reaching up to 2x better lateral and axial resolution compared to standard widefield imaging
  • Super-resolution 2D images at high speed captured by „TIRF-SIM“ mode for excitation wavelengths 488 and 561 nm for better understanding of molecular interactions at the cell surface
  • Two-color super-resolution images obtained by single molecule localization methods (STORM, dSTORM, PALM; TIRF or HILO illumination available) with resolution improvement up to 10x compared to conventional optical microscopes
  • Fast and sensitive multicolor widefield imaging with TIRF or HILO excitation option
  • Option of simultaneous dual wavelength imaging by single camera 
  • Long term live-cell imaging available
  • Brightfield microscopy

Specifications

Inverted widefield microscope Nikon Eclipse Ti-E equipped with a piezo Z-stage, motorized XY stage, Perfect Focus System, automatized H-TIRF module, insertable quarter-wave plate, insertable gradation neutral density filter, transmitted light lamp (100 W) and following units:

Software
NIS-Elements (v5.21)
Epifluorecence
Nikon Intensilight E
Laser excitation wavelengths
405 nm, 445nm, 488 nm, 561 nm, 647 nm (all cw)
Objectives
  • Nikon CFI HP Apo TIRF 100x Oil, NA 1.49, WD 0.12 mm, Temperature Correction Ring 23-37°C (for STORM)
  • Nikon CFI SR Apo TIRF 100x Oil, NA 1.49, WD 0.12 mm, Temperature Correction Ring 23-37°C (for SIM)
  • Nikon CFI Plan Apo 60x WI, NA 1.27, WD 0.17 mm, Correction Collar 0.15-0.19 (for SIM)
  • Nikon CFI Plan Apo Lambda 20x, NA 0.75, WD 1mm
Cameras
EM CCD Andor iXon Ultra DU897 (Andor Technologies)
  • right port
  • available for both SIM and localization super-resolution techniques, live-cell imaging and TIRF imaging
  • 512x512 pixels
  • pixel size: 16x16 μm
  • frame rate 56 fps for 512x512 pixels, standard mode
  • frame rate up to 1 fps for 512x512 pixels, 3D-SIM acquisition
  • frame rate up to 1.6 fps for 512x512 pixels, TIRF-SIM acquisition

sCMOS Hamamatsu ORCA 4.0 V2 (Hamamatsu Photonics)
  • left port
  • available for both localization super-resolution techniques, live-cell imaging and TIRF imaging
  • 2048x2048 pixels
  • pixel size: 6.5x6.5 μm
  • frame rate 100 fps for 2048x2048 pixels, standard scan
Filter turret 1
DAPI (361-389, DM415, 430-490)
CFP TIRF (436/20, 442rdc, 460lp, 480/40) – inserted on demand
488 TIRF (488/10, 488rdc, 500lp, 525/50)
561 TIRF (561/10, 561rdc, 575lp, 600/50)
Cy5 (628/40, DM660, 731/137) – inserted on demand
continuous STORM TIRF
(excitation, mirror, emission:
387-417, 420-481, 422-478
483-494, 497-553, 502-549
557-570, 575-628, 581-625
636-661, 667-792, 674-786)
HM Half Mirror
Filter turret 2
SIM488 (470-490, DM515, 500-545)
SIM561 (556-566, DM590, 570-640)
SIM647 (590-650, DM665, 663-738)
HM Half Mirror
Tube lenses
1x, 1.5x
Relay lenses in front of ORCA Camera
1x (widefield), 0.4x (STORM)
Relay lenses in front of EM CCD Camera
1x (astigmatism lens for STORM)
1x (widefield)
2.5x (SIM)
Astigmatism lens (for 3D STORM)
Yes, insertion possible for both cameras
SIM diffraction grating blocks
3D EX V-R (3D-SIM 100x oil objective)
3D EX V-R (3D-SIM 60x water objective)
TIRF 488 (TIRF 100x oil objective)
TIRF 561 (TIRF 100x oil objective)
Image splitter
DV2 (Photometrics)
  • inserted on demand
  • available beamsplitters: 565 nm (T565lpxr), 505 nm (T505lpxr), Polarization/Anisotropy module

W-VIEW GEMINI (Hamamatsu Photonics)
  • inserted on demand
  • available beamsplitters for 484 nm, 560 nm, 640 nm, and 700 nm
  • Inverted widefield microscope
  • TIRF illumination
  • Image splitter

Versatile fluorescence inverted widefield microscope equipped with four lasers, epifluorescence lamp and EM-CCD and CMOS cameras. The microscope is designed for short and long live-cell imaging and experiments under standard or TIRF illumination. Link to manufacturer website:

https://www.nikoninstruments.com/cz_CZ/Vyrobky/Inverzni-mikroskopy/Eclipse-Ti-E

Applications

  • Fast and sensitive multicolor widefield imaging with TIRF, HILO or EPI excitation option
  • Option of simultaneous dual wavelength imaging by single camera 
  • Short or long term live-cell imaging available
  • Various measurement options: z-stack, time series, tile scan, multi positions
  • Brightfield and DIC microscopy

Specifications

Inverted widefield microscope Nikon Eclipse Ti-E equipped with motorized XY stage, Perfect Focus System, H-TIRF module, module for environmental control Okolab (temperature controlled range room temperature up to 40°C; CO2 0-10%; humidity up to 95 %), insertable quarter-wave plate, insertable gradation neutral density filter, transmitted light lamp (100 W) and following units:

Software
NIS-Elements Ar (v5.02)
Epifluorecence
Nikon Intensilight E
Laser excitation wavelengths
405 nm, 488 nm, 561 nm, 640 nm (all cw)
Objectives
  • Nikon CFI Apo TIRF 60x Oil, NA 1.49, WD 0.12 mm, Correction Collar, DIC (motorized prism insertion)
  • Nikon CFI Plan Apo 60x WI, NA 1.27, WD 0.17 mm, Correction Collar 0.15-0.19, DIC
  • Nikon CFI Plan Apo Lambda 20x, NA 0.75, WD 1mm, DIC
  • Nikon CFI Plan Apo Lambda 10x, NA 0.45, WD 4 mm
Cameras
EM CCD Andor iXon Ultra DU888 (Andor Technologies)
  • right port
  • 1024 x 1024 pixels
  • pixel size: 13 μm x 13 μm
  • 26 fps at 1024 x 1024 pixels, QEmax> 90%

CMOS Hamamatsu ORCA-flash4.0 LT (Hamamatsu Photonics)
  • left port
  • 2048 x 2048 pixels
  • pixel size: 6.5 x 6.5 μm
  • 30 fps 2048 x 2048 pixels
Filter turret
TagBFP (excitation 395-415, emission 435-485)
EGFP (483-493, 500-550)
mCherry (556-566, 593-668)
Cy5 (632-652, 669-741)
Quad Band Set - 405/488/561/640
Tube lenses
1x, 1.5x
Relay lens in front of EM CCD Camera
2.5x
Image splitter
W-View Gemini (Hamamatsu Photonics)
  • in front of Hamamatsu camera
  • available beamsplitters: 484 nm, 560 nm, 640 nm and 700 nm
  • Inverted widefield microscope
  • TIRF illumination

Versatile fluorescence inverted widefield microscope equipped with three lasers, epifluorescence lamp, and back-illuminated sCMOS camera. The microscope is designed for short- and long-term imaging experiments under standard or TIRF illumination. Links to manufacturer website:

https://www.microscope.healthcare.nikon.com/products/inverted-microscopes/eclipse-ti2-series

Applications

  • Fast and sensitive multicolor widefield imaging with TIRF, HILO or EPI excitation option
  • Various measurement options: z-stack, time series, tile scan, multi positions
  • Brightfield and DIC microscopy

Specifications

Inverted widefield microscope Nikon Eclipse Ti2 equipped with motorized XY stage, Perfect Focus System, H-TIRF module, insertable quarter-wave plate, insertable gradation neutral density filter, transmitted light lamp (Nikon TI2-D-LHLED) and following units:

Software
NIS-Elements AR (v. 5.20)
Epifluorecence
CoolLED pE-300, 400 nm, 460 nm, 550 nm
Laser excitation wavelengths
488 nm, 561 nm, 640 nm (all cw)
Filter turret
EM435-485
EM500-545
EM610/75
EM700/75
Quad Band Set - 405/488/561/640
Objectives
  • Nikon Plan Apo λ 20x, NA 0.75, WD 1.00 mm
  • Nikon Apo TIRF 60x Oil, NA 1.49, WD 0.12 mm, Correction Collar 0.15-0.19, DIC
Tube lenses
1x, 1.5x
Relay lens (mountable)
2.5x
Camera
PRIME BSI (Teledyne Photometrics)
  • 2048 x 2048 pixels
  • pixel size: 6.5 x 6.5 μm
  • 63 fps @ 2048 x 2048 pixels, 11-bit data
  • QEmax> 95%
  • Upright confocal microscope
  • Internal spectral detection unit

An upright fluorescence confocal microscope equipped with four excitation lasers, dipping objectives with long working distance, oil immersion objective and 1x HyD detector and 2x PMTs. Presence of camera, epifluorescence lamp and removable infrared filter allows also widefield imaging in infrared area. Accessory for electrophysiological experiments is also available. For more detailed microscope characteristics please see the section “Specifications”.

Applications

  • Fast and multi-color confocal imaging
  • Fast and multi-color widefield imaging 
  • Various measurement options: z-stack, time series
  • Spectral imaging, measurement of emission spectrum
  • Option of infrared detection in widefield imaging  
  • Brightfield and polarized light microscopy

Specifications

An upright confocal microscope DM6 CFS with a laser scanning confocal head Leica TCS SP8, a manual microscope stage, Best focus control, transmitted light LED U-12V, removable infrared filter, square pinhole of tunable size and following units:

Software
LAS-X
Epifluorescence lamp
Leica EL6000
Lasers
405, 488, 552, 638 nm (all cw)
Beamsplitters
DD 488/552
TD 488/552/638
RT 15/85
Filter turret
– filter cubes for epifluorescence lamp
DAPI    (Ex: 325-375; DM: 400; Em: 435-485)
FITC     (Ex: 460-500; DM: 505; Em: 512-542)
RHOD (Ex: 541-551; DM: 560; Em: 565-605)
CY5       (Ex: 590-650; DM: 660; Em: 662-738) – not inserted
Analyser
Objectives
  • HC APO L 10x (W), NA 0.30, WD 3.7 mm, dipping objective
  • HC FLUOTAR L 25x (W), NA 0.95, WD 2.5 mm, dipping objective
  • HC PL APO CS2 63x Oil, NA 1.40, WD 0.14 mm

Two objectives are installed at maximum.
Scanner
FOV SP8 scanner
  • maximal sampling 8192 x 8192 px
  • speed 1-1800 Hz (7fps@512 x 512 px)
  • hardware zoom 0.75x-48x
Detection
Transmitted light detector (PMT)
Internal spectrally tunable detection unit
  • 1x HyD detectors (GaAsP, 400 – 720 nm, Modes: Standard, Counting, BrightR), QE=45%@500nm
  • 2x PMT detectors (400-800nm), QE=30%@500nm
Camera DFC365 FX (Leica)
  • 1392 x 1040 pixels
  • 6.45 μm x 6.45 μm
  • CCD sensor Sony ICX285
  • C-mount 0.7x
  • Inverted widefield microscope

Inverted widefield fluorescence microscope equipped with epifluorescence lamp and EM-CCD camera.

Applications

  • Fast and multi-color widefield imaging
  • Various measurement options: z-stack, time series, tile scan, multi positions
  • Brightfield and DIC microscopy

Specifications

An inverted widefield microscope Dmi8 with motorized microscope stage, transmitted light LED U-12V, and following units:

Software
LAS-X, Navigator
Epifluorescence lamp
CoolLED pE-300
Filter turret
DAPI    (Ex: 325-375; DM: 400; Em: 435-485)
FITC     (Ex: 460-500; DM: 505; Em: 512-542)
RHOD (Ex: 541-551; DM: 560; Em: 565-605)
CY5       (Ex: 590-650; DM: 660; Em: 662-738)
Analyser
Objectives
  • HC PL FLUOTAR 10x, NA 0.30, WD 11 mm
  • HC PL FLUOTAR L 20x, NA 0.40, WD 6.9 mm
  • HC PL FLUOTAR L 40x, NA 0.60, WD 3.3 mm
  • HC PL APO 63x Oil, NA 1.4, WD 0.14 mm, DIC
Camera: left port
ORCA-Flash4.0 V3 (Hamamatsu)
  • monochrome digital sCMOS camera
  • 2048 x 2048 pixels
  • pixel size: 6.5x6.5 μm
  • readout rate 40 fps for 16bit full frame
Camera: right port
K3C (Leica)
  • color digital CMOS camera
  • 3072 x 2048 pixels
  • pixel size: 2.4 x 2.4 μm
  • bit depth: 3x12bit RGB
  • readout rate 15 fps
  • Upright macroscope

Fluorescence zoom upright microscope which combines the benefits of stereomicroscopes such as zoom optics and long working distances with the higher resolutions of traditional light microscopes. The macroscope in IMCF at BIOCEV is equipped with a 2.3x magnification objective combined with numerical aperture 0.57 what offers superior brightness in large objects fields. In comparable image fields, the Axio Zoom.V16 offers a 2.5 times higher resolution and 10 times brighter fluorescence than generally used stereomicroscopes. Link to manufacturer web:

http://www.zeiss.com/microscopy/en_de/products/stereo-zoom-microscopes/axio-zoom-v16-for-materials.html

Applications

  • visualization of large samples, e.g. whole model organism, in fluorescence contrast or transmitted light with the possibility to zoom in to the smallest details of the sample. Long working distance of the available objectives allows comfortable manipulation with specimens. 
  • basic documentation of histological samples.

Specifications

Axiozoom.V16 is a fluorescence zoom upright microscope equipped with a motorized stage with travel range 150 x 100 mm and the following:

Software
ZEN blue pro 2012
Illumination
  • Transmitted light – Zeiss CL 9000 LED CAN with day-light filter for cold light LED
  • Fluorescence - HXP 120 V (D) – long-life, metal halide illuminator
Filter cubes
Filter set 49 DAPI    (Ex: 365; DM: 395; Em: 445/450)
Filter set 38 GFP      (Ex: 470/40; DM: 495; Em: 525/550)
Filter set 63 RFP      (Ex: 572/25; DM: 590; Em: 629/62)
Filter set 50 CY5      (Ex: 640/30; DM: 660; Em: 690/50)

All filter sets are exchangeable.
Objectives
Objective TypeImmersionMagnificationN.A.WD[mm]DIC/Correct. Ring
PlanNeoFluar ZAir1x0.2556-
PlanNeoFluar ZAir2.3x0.5710.6-
Detection
Camera Axiocam 506 mono
  • 6 Mpix (2752x2208)
  • pixel size 4,54 μm x 4.54 μm
  • 19 fps at full frame
  • effective sensor size 12.5 x 10.0 mm
  • 3D holotomography & fluorescence
  • Stage-top incubator for long-term automatic live cell imaging

Automated holotomographic and fluorescence microscope for long-term live-cell imaging. Label-free 3D imaging of cells in real time is ensured due to the low power laser and top stage. In addition, presence of epifluorescence lamp allows correlative holographic and fluorescence imaging. Link to manufacturer website:

https://www.nanolive.ch/products/3d-microscopes/cx-a/

Applications

  • Gentle long-term live-cell label-free 3D imaging
  • Correlative holographic (3D) and fluorescence (2D) imaging
  • Imaging of cell populations, single cells or organelles
  • Measurement of refractive indices inside the cell

Specifications

Software
EVE - data acquisition
STEVE - data visualization and post-processing
Illumination
520 nm (Class 1 laser low power, sample exposure 0.2 mW/mm2)
Epifluorescence lamp
CoolLED pE-300
Excitation filters
392/23, 474/27, 554/23 or 635/18
Emission filters
For DAPI, FITC, TRITC/Cy5 imaging (Semrock FF01-432/515/595/730)
Objective
Dry objective 60x, NA 0,8
Camera
USB 3.0 CMOS Sony IMX174 sensor
  • Quantum Efficiency 70 % (at 545 nm)
  • Dark Noise 6,6 e¯
  • Dynamic Range 73,7 dB
Module for environmental control
Tokaihit stage top incubator
  • CO2 concentration range: 5% - 20% (±0.1%)
  • Humidity: ~ 95%
  • Sample temperature: 30-40°C (±0.3°C)

Sample insert
4 dish format
96 well plate
Recommended labware
35 mm low IBIDI dish Cat.No: 80136; optional DIC lid Cat.No: 80050; recommendations: respect dish volume (1-2 ml), use same volume in each well, assure no meniscus, assure no bubbles, change medium before imaging)
Nanolive 96-well format plates – recommendations: respect well volume (80-90μl), each well must have the same volume
  • cytometry & fluorescence

Fully automated imaging cytometer designed for fast acquisition and analysis of robust data volume by advanced methods of multiparametric cytometric analysis, reconstruction and multidimensional visualization. Link to manufacturer website:

https://www.moleculardevices.com/systems/high-content-imaging/imagexpress-micro-xls-widefield-high-content-analysis-system

Applications

  • localization, quantification and functional analysis of mitochondria in cell, effectivity and dynamics of internalization of surface structures, viability and morphology of cells or subcellular structures
  • cytotoxicity, apoptosis/necrosis
  • cell cycle and mitosis
  • cell differentiation, number, viability and phosphorylation of cells or subcellular structures, activation/inhibition of signal pathways and phosphorylation of proteins, effectivity of transfection, cell migration and analysis of objects movement
  • effectivity and dynamics of internalization of surface structures, localization and co-localization of proteins in nuclear, perinuclear, cytoplasmic or membrane localization

Specifications

Automatic hardware autofocus via IR laser. Automatic software autofocus.

Software
1 offline licence
Modules:
  • Granularity Application Module
  • Live/Dead Application Module
  • Cell cycle Application Module
  • Multiwavelenght Cell Scoring Application Module
  • Multiwavelenght Translocation Application Module
Epifluorecence
solid state with spectral range 400-640nm and 10 000 hours life time
Objectives
  • 10x Plan Fluor, NA 0.3
  • 20x Ph1 S Plan Fluor ELWD ADM, NA 0.45, appropriate for phase contrast
  • 40x Ph2 S Plan Fluor ELWD ADM, NA 0.60, appropriate for phase contrast
Filters
DAPI
eGFP/FITC
TRITC
Detector
SCMOS
4.5 MPx, 80-200 frames per sec., 16bit A/D converter, dynamic range 1:20000, low electronic noise 1.4 e/px
Stage
inserts for multiwall plates (6 well – 1536 well)
microscopic slides (possible up to 3 slides simultaneously)
adjustable in X, Y, Z with 100nm accuracy