We cover the following methods:

• wide-field microscopy,
• confocal microscopy,
• two-photon deep intravital microscopy including SHG and THG,
• 3D super-resolution microscopy (STED, STORM, SIM),
• functional imaging (spectral, FLIM, FCS),
• TIRF,
• live cell imaging.

The strength of our facility is the application, implementation, and development of advanced fluorescence imaging techniques, especially super-resolution and functional (FLIM, FCS) imaging.

Acknowledgment

We acknowledge Imaging Methods Core Facility at BIOCEV, institution supported by the MEYS CR (Large RI Project LM2018129 Czech-BioImaging) and ERDF (project No. CZ.02.1.01/0.0/0.0/18_046/0016045) for their support with obtaining imaging data presented in this paper.