Electron microscopy

We cover the following methods:

  • Consultation on sample preparation, harvesting and controls
  • Optimization of sample processing protocols and workflows
  • Standard (chemical) sample preparation including Microwave processing
  • Cryo-processing by High Pressure Freezing and Automated Freeze-Substitution
  • Negative staining and Automated Plunge freezing followed by RT-TEM or cryo-TEM analysis
  • TEM analysis of ultra-thin sections from resin-embedded cells, tissues and bio-composites
  • 3D-reconstruction of sub-cellular volumes by Electron Tomography
  • Antigen detection and localization by immuno-gold TEM/SEM
  • Electron Diffraction of microcrystalline compounds
  • Surface morphology of biological samples and materials by SEM
  • Elemental analysis by EDS (in SEM)
  • 3D Ultrastructural analysis by FIB-SEM
  • Correlative Light and Electron Microscopy (CLEM)
  • EM data processing and quantitative analysis
  • User training and support

Samples we analyze:

  • Protein and macromolecular complexes
  • Viruses, nanoparticles, liposomes
  • Bacteria and yeast cells
  • Adherent and suspension cells
  • Tissues
  • Organoids and small organisms
  • Bio-composites and materials

Acknowledgment

We acknowledge Imaging Methods Core Facility at BIOCEV, institution supported by the MEYS CR (Large RI Project LM2018129 Czech-BioImaging) and ERDF (project No. CZ.02.1.01/0.0/0.0/18_046/0016045) for their support with obtaining imaging data presented in this paper.