We cover the following methods:
- Consultation on sample preparation, harvesting and controls
- Optimization of sample processing protocols and workflows
- Standard (chemical) sample preparation including Microwave processing
- Cryo-processing by High Pressure Freezing and Automated Freeze-Substitution
- Negative staining and Automated Plunge freezing followed by RT-TEM or cryo-TEM analysis
- TEM analysis of ultra-thin sections from resin-embedded cells, tissues and bio-composites
- 3D-reconstruction of sub-cellular volumes by Electron Tomography
- Antigen detection and localization by immuno-gold TEM/SEM
- Electron Diffraction of microcrystalline compounds
- Surface morphology of biological samples and materials by SEM
- Elemental analysis by EDS (in SEM)
- 3D Ultrastructural analysis by FIB-SEM
- Correlative Light and Electron Microscopy (CLEM)
- EM data processing and quantitative analysis
- User training and support
Samples we analyze:
- Protein and macromolecular complexes
- Viruses, nanoparticles, liposomes
- Bacteria and yeast cells
- Adherent and suspension cells
- Tissues
- Organoids and small organisms
- Bio-composites and materials
Acknowledgment
We acknowledge Imaging Methods Core Facility at BIOCEV, institution supported by the MEYS CR (Large RI Project LM2018129 Czech-BioImaging) and ERDF (project No. CZ.02.1.01/0.0/0.0/18_046/0016045) for their support with obtaining imaging data presented in this paper.