Publications of IMCF users


Arbon, D. et al. Adaptive iron utilization compensates for the lack of an inducible uptake system in naegleria fowleri and represents a potential target for therapeutic intervention. PLoS Negl. Trop. Dis. 14, 1–25 (2020).
Filova, I. et al. Combined Atoh1 and Neurod1 Deletion Reveals Autonomous Growth of Auditory Nerve Fibers. Mol. Neurobiol. (2020) doi:10.1007/s12035-020-02092-0.
Frolikova, M. et al. The role of taste receptor mTAS1R3 in chemical communication of gametes. Int. J. Mol. Sci. 21, (2020).
Henrichs, V. et al. Mitochondria-adaptor TRAK1 promotes kinesin-1 driven transport in crowded environments. Nat. Commun. 11, 3123 (2020).
Horníková, L. et al. The Major Capsid Protein, VP1, of the Mouse Polyomavirus Stimulates the Activity of Tubulin Acetyltransferase 1 by Microtubule Stabilization Lenka. Viruses 12, (2020).
Hubackova, S. et al. Replication and ribosomal stress induced by targeting pyrimidine synthesis and cellular checkpoints suppress p53-deficient tumors. Cell Death Dis. 11, (2020).
Jankovicova, J. et al. Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells. Sci. Rep. 10, 1–12 (2020).
Krčová, L. et al. Highly selective mitochondrial probes based on fluorinated pentamethinium salts: On two-photon properties and microscopic applications. Dye. Pigment. 172, (2020).
Kusari, F., Mihola, O., Schimenti, J. C. & Trachtulec, Z. Meiotic epigenetic factor PRDM9 impacts sperm quality of hybrid mice. Reproduction 160, 53–64 (2020).
Le, T. et al. Anaerobic peroxisomes in Mastigamoeba balamuthi. Proc. Natl. Acad. Sci. U.S.A. 117, 2065–2075 (2020).
Ma, Y. et al. Clustering of the ζ-chain can initiate t cell receptor signaling. Int. J. Mol. Sci. 21, 1–25 (2020).
Rabata, A., Fedr, R., Soucek, K., Hampl, A. & Koledova, Z. 3D Cell Culture Models Demonstrate a Role for FGF and WNT Signaling in Regulation of Lung Epithelial Cell Fate and Morphogenesis. Front. Cell Dev. Biol. 8, 1–16 (2020).
Sklenář, F. et al. Increasing the species diversity in the Aspergillus section Nidulantes: Six novel species mainly from the indoor environment. Mycologia 112, 342–370 (2020).
Špaček, M. et al. Comparison of Different Thawing Protocols in Human Cryopreserved Venous Grafts. Ann. Vasc. Surg. 64, 347–354 (2020).
Talianová, V. et al. Coumarin Tröger’s base derivatives with cyanine substitution as selective and sensitive fluorescent lysosomal probes. Bioorg. Chem. 94, 103447 (2020).
Ustinova, K. et al. The disordered N-terminus of HDAC6 is a microtubulebinding domain critical for efficient tubulin deacetylation. J. Biol. Chem. 295, 2614–2628 (2020).
Vaitkinene, S., Daugelavičius, R., Sychrová, H. & Kodedová, M. Styrylpyridinium Derivatives as New Potent Antifungal Drugs and Fluorescence Probes. Front. Microbiol. 11, 1–14 (2020).
Valdinocci, D., Kovarova, J., Neuzil, J. & Pountney, D. L. Alpha-Synuclein Aggregates Associated with Mitochondria in Tunnelling Nanotubes. Neurotox. Res. (2020).


Barinka, C. et al.: Structural and computational basis for potent inhibition of glutamate carboxypeptidase II by carbamate-based inhibitors. Bioorganic Med. Chem. 27, 255–264 (2019).
Bohuslavova, R. et al.: HIF-1α is required for development of the sympathetic nervous system. Proc. Natl. Acad. Sci. U. S. A. (2019) doi:10.1073/pnas.1903510116.
Dlasková, A. et al.: Mitochondrial cristae narrowing upon higher 2-oxoglutarate load. Biochim. Biophys. Acta - Bioenerg. 1860, 659–678 (2019).
Fawley, M. W., Němcová, Y. & Fawley, K. P.: Phylogeny and characterization of paraeustigmatos columelliferus, gen. Et sp. nov., a member of the eustigmatophyceae that may represent a basal group within the eustigmatales. Fottea 19, 107–114 (2019).
Figura, T., Tylová, E., Šoch, J., Selosse, M. A. & Ponert, J.: In vitro axenic germination and cultivation of mixotrophic Pyroloideae (Ericaceae) and their post-germination ontogenetic development. Ann. Bot. 123, 625–639 (2019).
Frolikova, M. et al.: Addressing the Compartmentalization of Specific Integrin Heterodimers in Mouse Sperm. Int. J. Mol. Sci. 20, 1–16 (2019).
Gemperle, J., Dibus, M., Koudelková, L., Rosel, D. & Brábek, J.: The interaction of p130Cas with PKN3 promotes malignant growth. Mol. Oncol. 13, 264–289 (2019).
Hernychová, L. et al.: The C-type lectin-like receptor Nkrp1b: Structural proteomics reveals features affecting protein conformation and interactions. J. Proteomics 196, 162–172 (2019).
Havlík, M. et al.: Versatile fluorophores for bioimaging applications: π-expanded naphthalimide derivatives with skeletal and appendage diversity. Chem. Commun. 55, 2696–2699 (2019).
Kohutova, J. et al.: Anti-arrhythmic cardiac phenotype elicited by chronic intermittent hypoxia is associated with alterations in connexin-43 expression, phosphorylation, and distribution. Front. Endocrinol. (Lausanne). 10, 1–10 (2019).
Koudelkova, L. et al.: Novel FRET-Based Src Biosensor Reveals Mechanisms of Src Activation and Its Dynamics in Focal Adhesions. Cell Chem. Biol. 1–14 (2019).
Karnkowska, A. et al.: The Oxymonad Genome Displays Canonical Eukaryotic Complexity in the Absence of a Mitochondrion. Mol. Biol. Evol. (2019) doi:10.1093/molbev/msz147.
Krejcir, R. et al.: A cyclic pentamethinium salt induces cancer cell cytotoxicity through mitochondrial disintegration and metabolic collapse. Int. J. Mol. Sci. 20, (2019).
Makki, A. et al.: Triplet-pore structure of a highly divergent TOM complex of hydrogenosomes in Trichomonas vaginalis. PLOS Biology vol. 17 (2019).
Musilova, Z. et al. Evolution of the visual sensory system in cichlid fishes from crater lake Barombi Mbo in Cameroon. Mol. Ecol. (2019) doi:10.1111/mec.15217.
Oulehlová, D. et al.: Arabidopsis Class I Formin FH1 Relocates between Membrane Compartments during Root Cell Ontogeny and Associates with Plasmodesmata. Plant Cell Physiol. 60, 1855–1870 (2019).
Meiring, J. C. M. et al.: Colocation of Tpm3.1 and myosin IIa heads defines a discrete subdomain in stress fibres. J. Cell Sci. 132, (2019).
Palma-Onetto, V. et al.: The labral gland in termites: Evolution and function. Biol. J. Linn. Soc. 126, 587–597 (2019).
Princová, J., Schätz, M., Ťupa, O. & Převorovský, M.: Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing. J. Vis. Exp. (2019) doi:10.3791/59889.
Pusztai, M. & Škaloud, P.: Elucidating the evolution and diversity of Uroglena-like colonial flagellates (Chrysophyceae): polyphyletic origin of the morphotype. Eur. J. Phycol. 54, 404–416 (2019).
Rada, P., Makki, A., Žárský, V. & Tachezy, J.: Targeting of tail-anchored proteins to Trichomonas vaginalis hydrogenosomes. Mol. Microbiol. 111, 588–603 (2019).
Rada, P., Kellerová, P., Verner, Z. & Tachezy, J.: Investigation of the Secretory Pathway in Trichomonas vaginalis Argues against a Moonlighting Function of Hydrogenosomal Enzymes. J. Eukaryot. Microbiol. 66, 899–910 (2019).
Rubešová, K. et al.: Heavily Ce3+-doped Y3Al5O12 thin films deposited by a polymer sol-gel method for fast scintillation detectors. CrystEngComm 21, 5115–5123 (2019).
Štundlová, J., Šmíd, J., Nguyen, P. & Šťáhlavský, F.: Cryptic diversity and dynamic chromosome evolution in Alpine scorpions (Euscorpiidae: Euscorpius). Mol. Phylogenet. Evol. 134, 152–163 (2019).
Siahaan, V. et al.: Kinetically distinct phases of tau on microtubules regulate kinesin motors and severing enzymes. Nat. Cell Biol. 21, 1087- (2019).
Schwarzerová, K. et al.: Tubulin is actively exported from the nucleus through the Exportin1/CRM1 pathway. Sci. Rep. 9, 1–13 (2019).
Schmidt-Cernohorska, M. et al.: Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails. Science. 363, 285–288 (2019).
Treitli, S. C., Kolisko, M., Husník, F., Keeling, P. J. & Hampl, V.: Revealing the metabolic capacity of Streblomastix strix and its bacterial symbionts using singlecell metagenomics. Proc. Natl. Acad. Sci. U. S. A. 116, 19675–19684 (2019).
Vennin, C. et al.: CAF hierarchy driven by pancreatic cancer cell p53-status creates a pro-metastatic and chemoresistant environment via perlecan. Nat. Commun. (2019) doi:10.1038/s41467-019-10968-6.


Bajzikova, M., Kovarova, J., Coelho, A. R. et al.: Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells. Cell Metab (2018).
Baresova, V., Skopova, V., Souckova, O. et al.: Study of Purinosome Assembly in Cell-based Model Systems with De Novo Purine Synthesis and Salvage Pathway Deficiencies. PLoS One 13, 1-13 (2018).
Cerychova, R., Bohuslavova, R., Papousek, F et al.: Adverse Effects of Hif1a Mutation and Maternal Diabetes on the Offspring Heart Cardiovas Diabetol 17, 1-16 (2018).
Font-Haro, A. et al.: Expression of TIM-3 on Plasmacytoid Dendritic Cells as a Predictive Biomarker of Decline in HIV-1 RNA Level During ART. Viruses 3, 1-13 (2018).
Frolikova, M., Manaskova-Postlerova, P., Cerny, J. et al.: CD9 and CD81 Interactions and their Structural Modelling in Sperm Prior to Fertilization. Intern Journal of Mol Sci 19, 4-17 (2018).
Grzelak, A., Polakova, I., Smahelova, J. el al.: Experimental Combined Immunotherapy of Tumours with Major Histocompatibility Complex Class I Downregulation. Intern Journal of Molec Sci 19, 3693 (2018).
Hubackova, S., Davidova, E., Rohlenova, K. et al.: Selective Elimination of Senescent Cells by Mitochondrial Targeting is Regulated by ANT2. Cell Death and Differ, 1-15 (2018).
Janovec, V., Aouar, B., Font-Haro, A. et al.: The MEK1/2-ERK Pathway Inhibits Type I IFN Production in Plasmacytoid Dendritic Cells. Front In Immunol 9, 1-16 (2018).
Kazamia, E., Sutak, R., Paz-Yepes, J. et al.: Endocytosis-mediated Siderophore Uptake as a Strategy for Fe Acquisition in Diatoms. Science Advances 4 (2018).
Kollarova, J., Kostrouchova, M., Benda, A. and Kostrouchova, M.: ALKB-8, a 2-Oxoglutarate-Dependent Dioxygenase and S-Adenosine Methionine-Dependent Methyltransferase Modulates Metabolic Events Linked to Lysosome-Related Organelles and Aging in C. elegans. Folia Biol. (Praha). 64, 46–58 (2018).
Kostrouchova, M., Kostrouchova, V., Yilma, P. et al.: Valproic Acid Decreases the Nuclear Localization of MDT-28, the Nematode Orthologue of MED28. Folia Biologica 64, 1-19 (2018).
Mach, J., Bila, J., Zeniskova, K. et al.: Iron Economy in Naegleria Gruberi Reflects its Metabolic Flexibility. Intern Journal for Parasitol 48, 719-727 (2018).
Rada, P., Makki, A., Zarsky, V. and Tachezy, J.: Targeting of Tail-Anchored Proteins to Trichomonas Vaginalis Hydrogenosomes. Mol Microbiol (2018).
Stafkova, J., Rada, P., Meloni, D. et al.: Dynamic Secretome of Trichomonas Vaginalis : Case Study of β-amylases. Mol & Cell Proteom 17, 304-320 (2018).
Becker, W., Studier, H., Wetzker, C. and Benda, A.: Ultra-fast HPM Detectors Improve NAD(P)H FLIM. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 10498 (2018).
Ma, Y., Benda, A., Kwiatek, J. et al.: Time-Resolved Laurdan Fluorescence Reveals Insights into Membrane Viscosity and Hydration Levels. Biophys Journal (2018).
Nicovich, P. R., Kwiatek, J. M., Ma, Y. et al.: FSCS Reveals the Complexity of Lipid Domain Dynamics in the Plasma Membrane of Live Cells. Biophys Jour 114, 2855-2864 (2018).
Olsinova, M., Jurkiewicz, P., Kishko, I. et al.: Roughness of transmembrane helices reduces lipid membrane dynamics. iScience 10, 87–97 (2018).
Proksova, P., Lipov, J., Zelenka, J. et al.: Mason-Pfizer Monkey Virus Envelope Glycoprotein Cycling and Its Vesicular Co-Transport with Immature Particles. Viruses 10, 575 (2018).
Shestivska, V., Olsinova, M., Sovova, K. et al.: Evaluation of Lipid Peroxidation by the Analysis of Volatile Aldehydes in the Headspace of Synthetic Membranes Using Selected Ion Flow Tube Mass Spectrometry. Rapid Commun in Mass Spectrometry 32, 1617-1628 (2018).
Allolio, C., Magarkar, A., Jurkiewicz, P. et al.: Arginine-rich Cell-penetrating Peptides Induce Membrane Multilamellarity and Subsequently Enter via Formation of a Fusion Pore. PNAS (2018), 201811520.
Cermak, V. et al.: RNA-seq of Macrophages of Amoeboid or Mesenchymal Migratory Phenotype Due to Specific Structure of Environment. Sci. Data 5, 1–8 (2018).
Gemperle, J., Dibus, M., Koudelkova, L., Rosel, D. and Brabek, J.: The Interaction of p130Cas with PKN3 Promotes Malignant Growth. Mol. Oncol. 1–26 (2018).
Koudelkova, L. et al.: Novel FRET-Based Src Biosensor Reveals Mechanisms of Src Activation and Its Dynamics in Focal Adhesions. Cell Chem. Biol. 1–14 (2018).
Siahaan, V. et al.: Kinetically Distinct Phases of Tau on Microtubules Regulate Kinesin Motors and Severing Enzymes. BioRxiv (2018). doi:10.1101/424374
Soldatova, I., Prilepskaja, T., Abrahamyan, L., Forstova, J. and Huerfano, S.: Interaction of the Mouse Polyomavirus Capsid Proteins with Importins is Required for Efficient Import of Viral DNA Into the Cell Nucleus. Viruses 10, 1–22 (2018).
Tolde, O. et al.: Quantitative Phase Imaging Unravels New Insight Into Dynamics of Mesenchymal and Amoeboid Cancer Cell Invasion. Sci. Rep. 8, 1–13 (2018).
Agarwal, K. Machan, R. and Prasad, D.K: Non-heuristic Automatic Techniques for Overcoming Low Signal-to-Noise-Ratio bias of Localization Microscopy and Multiple Signal Classification Algorithm. Scientific Repors 8 (1), 2018.
Neburkova, J., Sedlak. F., Zackova Suchanova, J. et al.: Inhibitor-GCPII Interaction: Selective and Robust System for Targeting Cancer Cells with Structurally Diverse Nanoparticles. Mol Pharmaceut, 15 (8), 2932 – 2945 (2018).


Blecha, J. et al.: Antioxidant Defense in Quiescent Cells Determinates Selectivity of Electron Transport Chain Inhibition-Induced Cell Death. Free Radic Biol Med 112, 253-266 (2017).
Bohuslavova, R., Cerychova, R., Nepomucka, K. and Pavlinkova, G.: Renal injury is accelerated by global hypoxia-inducible factor 1 alpha deficiency in a mouse model of STZ-induced diabetes. BMC Endocr. Disord. 17, 1–12 (2017).
Branis, J. et al.: The Role of Focal Adhesion Anchoring Domains of CAS in Mechanotransduction. Sci Rep 7 (2017).
Danko, M., Hrdlovic, P., Martinicka, A., Benda, A. and Cigan, M.: Spectral Properties of Ionic Benzotristhiazole Based Donor-Acceptor NLO-phores in Polymer Matrices and their one- and two-photone Cellular Imaging Ability. Photochem Photobiol Sci 16 (2017).
Hornikova, L., Fraiberk, M., Man, P., Janovec, V., Forstova, J.: VP1, the Major Capsid Protein of the Mouse Polyomavirus, Binds Microtubules, Promotes their Acetylation and Block the Host Cell Cycle. FEBS J. 284, 301-323 (2017).
Huerfano, S., Ryabchenko, B., Spanielova, H. and Forstova, J.: Hydrophobic Domains of Mouse Polyomavirus Minor Capsid Proteins Promote Membrane Association and Virus Exit from the ER. FEBS J 284, 883-902 (2017).
Kellerova, P. and Tachezy, J.: Zootonic Trichomonas Tenas and the New Trichomonad Species, Trichomonas Brixi n.sp., from the Oral Cavities of Dogs and Cats. Int J Parasitol 47, 247-255 (2017).
Kokavec, J. et al.: The ISWI ATPase Smarca5 (Snf2h) is Required for Proliferation and Differenciation of Hematopoietic Stem and Progenitor Cells. Stem Cells 35, 1614-1623 (2017).
Kostrouchova, M. et al.: The Nematode Homologue of Mediator Complex Subunit 28 , F28F8.5, is a Critical Regulator of C. elegans Development. PeerJ 2017 (2017).
Luo, W. et al.: ARHGAP42 is Activated by Src-Mediated Tyrosine Phosphorylation to Promote Cell Motility . J Cell Sci 130, 2382-2393 (2017).
Novakova, Z. et al.: Novel Monoclonal Antibodies Recognizing Human Prostate-Specific Membrane Antigen (PSMA) as Research and Theranostic Tools. Prostate 77, 749–764 (2017).
Pavlinkova, G. et al.: Transgenerational inheritance of susceptibility to diabetes-induced male subfertility. Sci. Rep. 7, (2017).
Skultetyova, L. et al.: Human Histone Deacetylase 6 Shows Strong Preference for Tubulin Dimers Over Assebled Microtubules. Sci Rep 7 (2017).
Voleman, L. et al.: Giardia Intestinalis Mitosomes Undergo Synchronised Fission but not Fusion and are Constitutively Associated with the Endoplasmic Reticulum. BMC Biol 15 (2017).
Zackova Suchanova, N., Neburkova, J., Spanielova, H., Forstova, J. and Cigler, P.: Retargeting Polyomavirus-like Particles to Cancer Cells by Chemical Modification of Capsid Surface. Bioconug Chem. 28, 307-313 (2017).


Boukalova, S. et al.: Mitochondrial Targeting of Metformin Enhances its Activity against Pancreatic Cancer. Mol Cancer Ther. 15, 2875-2886 (2016).
Dvorakova, M. et al.: Incomplete and Delayed Sox2 Deletion Defines Residual Ear Neurosensory Development and Maintenance. Sci Rep 6, 1-16 (2016).
Jankovicova, J. et al.: Characterization of tetraspanin protein CD81 in mouse spermatozoa and bovine gametes. Reproduction (2016). doi:10.1530/REP-16-0304
Agarwal, K. & Macháň, R. Multiple signal classification algorithm for super-resolution fluorescence microscopy. Nat. Commun. 7, (2016).
Benda, A., Aitken, H., Davies, D. S., Whan, R. & Goldsbury, C. STED imaging of tau filaments in Alzheimer’s disease cortical grey matter. J. Struct. Biol. 195, (2016).
Jiang, Y. et al. Time-resolved fluorescence anisotropy study of organic lead halide perovskite. Sol. Energy Mater. Sol. Cells 151, (2016).
Ma, Y., Benda, A., Nicovich, P. R. & Gaus, K. Measuring membrane association and protein diffusion within membranes with supercritical angle fluorescence microscopy. Biomed. Opt. Express 7, (2016).
Macháň, R., Foo, Y. H. & Wohland, T. On the Equivalence of FCS and FRAP: Simultaneous Lipid Membrane Measurements. Biophys. J. 111, 152–161 (2016).
Yavas, S., Macháň, R. & Wohland, T. The Epidermal Growth Factor Receptor Forms Location-Dependent Complexes in Resting Cells. Biophys. J. 111, 2241–2254 (2016).