Practical Course

The course will focus on basic operations in cryogenic electron microscopy (cryoEM), cryoimaging of vitrified particulate specimens (e.g. microtubules, vesicles, viruses, etc.) and the possibility of correlation with cryogenic fluorescence microscopy (cryoFM). The lectures will cover the theoretical principles of EM/cryoEM and FM/cryoFM. During hands-on sessions, participants will gain practical experience with cryo-sample preparation techniques (Plunge Freezer, High Pressure Freezing) and cryo-imaging using confocal fluorescence microscope and transmission electron microscope. The course is particularly suitable for beginners in cryoEM with little or no experience in the field.

April 7 - 9, 2025

BIOCEV, Vestec

The course is organized by the Imaging Methods Core Facility as annual course.

The course is supported by the National Infrastructure for Biological and Medical Imaging (Czech-BioImaging, Ministry of Education, Youth and Sports – Large Research Infrastructure, LM2023050).