Practical Course
The fastest and most gentle 3D live-cell imaging on the Carl Zeiss Lattice Lightsheet 7, used for tracking the trafficking of fluorescently labeled proteins in living cells, will be complemented by FRAP on the Nikon iLas 2 ring-TIRF/FRAP and Nikon CSU-W1, as well as by FCS on the Leica TCS SP8 WLL SMD-FLIM for measuring protein diffusion in the plasma membrane of living cells. Finally, model systems of molecular motors walking along microtubules, visualized using the Nikon Ti-E H-TIRF, will demonstrate the benefits of single-molecule approaches for studying molecular motion.
April 28 - 30, 2025
BIOCEV, Vestec
This course includes theoretical and hands-on training in four complementary microscopy approaches for visualizing and measuring the lateral movement of molecules in living cells or model systems.
The microscopy techniques covered in the course:
- Lattice light-sheet microscopy (LLS)
- Fluorescence recovery after photobleaching (FRAP)
- Fluorescence correlation spectroscopy (FCS)
- Single-particle tracking (SPT)
The course is supported by the National Infrastructure for Biological and Medical Imaging (Czech-BioImaging, Ministry of Education, Youth and Sports – Large Research Infrastructure, LM2023050).