{"id":1846,"date":"2023-02-08T14:54:19","date_gmt":"2023-02-08T14:54:19","guid":{"rendered":"http:\/\/imcf.natur.cuni.cz\/IMCF\/?page_id=1846"},"modified":"2023-02-08T17:00:16","modified_gmt":"2023-02-08T17:00:16","slug":"flow-cytometry","status":"publish","type":"page","link":"https:\/\/imcf.natur.cuni.cz\/IMCF\/flow-cytometry\/","title":{"rendered":"Flow Cytometry"},"content":{"rendered":"<div class=\"wp-block-ub-content-toggle wp-block-ub-content-toggle-block\" id=\"ub-content-toggle-block-3b2ca8d7-7f0e-4f1d-8a92-b91697ca1b6e\" data-mobilecollapse=\"false\" data-desktopcollapse=\"true\" data-preventcollapse=\"false\" data-showonlyone=\"true\">\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-0-3b2ca8d7-7f0e-4f1d-8a92-b91697ca1b6e\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-3b2ca8d7-7f0e-4f1d-8a92-b91697ca1b6e\" style=\"color: #000000; \"><strong>BD LSRFortessaTM SORP<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-0-3b2ca8d7-7f0e-4f1d-8a92-b91697ca1b6e\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"209\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/bd_fortressa-regular280.jpg\" alt=\"\" class=\"wp-image-412\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Multicolour flow cytometry analysis<\/li>\n\n\n\n<li>5 spatially separated lasers<\/li>\n\n\n\n<li>18 fluorescent detectors<\/li>\n\n\n\n<li>Sample loading options (5ml tubes or 96-\/384-well plates) <\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p><strong>Description<\/strong><\/p>\n\n\n\n<p>The BD LSRFortessaTM SORP is a benchtop digital flow cytometer. Equipped with 5 spatially separated lasers, 18 fluorescent detectors and 2 light scatter detectors for fast multicolour analysis with accurate data acquisition rate at up to 40,000 events per second (with beads). The BD LSRFortessaTM has a BDTM High Throughput Sampler (HTS) for increased lab productivity by acquiring samples from a 96- or 384-well plates.<\/p>\n\n\n\n<p>Link to the manufacturer: https:\/\/www.bdbiosciences.com\/en-us<\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<p>Multicolour flow cytometry analysis<\/p>\n\n\n\n<p><strong>Specification<\/strong><\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-91b0a7dd-f20e-4383-95bd-4b4f44c07af3\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-91b0a7dd-f20e-4383-95bd-4b4f44c07af3\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-91b0a7dd-f20e-4383-95bd-4b4f44c07af3\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-91b0a7dd-f20e-4383-95bd-4b4f44c07af3\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-91b0a7dd-f20e-4383-95bd-4b4f44c07af3\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-56\" class=\"tablepress tablepress-id-56\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Excitation Optics<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-57\" class=\"tablepress tablepress-id-57\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Laser Wavelength [nm]<\/h5><\/td><td class=\"column-2\">355<\/td><td class=\"column-3\">405<\/td><td class=\"column-4\">488<\/td><td class=\"column-5\">561<\/td><td class=\"column-6\">640<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Laser Power [mW]<\/h5><\/td><td class=\"column-2\">20<\/td><td class=\"column-3\">50<\/td><td class=\"column-4\">100<\/td><td class=\"column-5\">50<\/td><td class=\"column-6\">40<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Emission Optics<\/h5><\/td><td class=\"column-2\">Forward Scatter Detector: photodiode with 488\/10 bandpass filter for the 488-nm laser<br \/>\nSide Scatter Detector: photomultipler with a 488\/10 bandpass filter for the 488-nm laser<br \/>\n<br \/>\nFluorescence Detectors and Filters:<br \/>\n\n<table id=\"tablepress-58\" class=\"tablepress tablepress-id-58\">\n<thead>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><\/td><th class=\"column-2\">BP<\/th><th class=\"column-3\">LP<\/th><th class=\"column-4\">ND<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>UV Laser 355<\/h5><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">Indo-1 blue<\/td><td class=\"column-2\">530\/30<\/td><td class=\"column-3\">505<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">Indo-1 violet<\/td><td class=\"column-2\">450\/50<\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Violet Laser 405<\/h5><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\">BV786<\/td><td class=\"column-2\">780\/60<\/td><td class=\"column-3\">750<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\">Qdot655<\/td><td class=\"column-2\">670\/30<\/td><td class=\"column-3\">630<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\">Qdot605<\/td><td class=\"column-2\">610\/20<\/td><td class=\"column-3\">600<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-12\">\n\t<td class=\"column-1\">Qdot585<\/td><td class=\"column-2\">585\/15<\/td><td class=\"column-3\">570<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-13\">\n\t<td class=\"column-1\">AmCyan<\/td><td class=\"column-2\">525\/50<\/td><td class=\"column-3\">505<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-14\">\n\t<td class=\"column-1\">Pacific Blue<\/td><td class=\"column-2\">450\/50<\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-15\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-16\">\n\t<td class=\"column-1\"><h5>Blue Laser 488<\/h5><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-17\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-18\">\n\t<td class=\"column-1\">PerCP-Cy5.5<\/td><td class=\"column-2\">710\/50<\/td><td class=\"column-3\">685<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-19\">\n\t<td class=\"column-1\">FITC<\/td><td class=\"column-2\">530\/30<\/td><td class=\"column-3\">505<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-20\">\n\t<td class=\"column-1\">SSC<\/td><td class=\"column-2\">488\/10<\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-21\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-22\">\n\t<td class=\"column-1\"><h5>YellGreen Laser 561<\/h5><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-23\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-24\">\n\t<td class=\"column-1\">PE-Cy7<\/td><td class=\"column-2\">780\/60<\/td><td class=\"column-3\">750<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-25\">\n\t<td class=\"column-1\">PE-Cy5.5<\/td><td class=\"column-2\">710\/50<\/td><td class=\"column-3\">685<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-26\">\n\t<td class=\"column-1\">PE-Cy5<\/td><td class=\"column-2\">670\/30<\/td><td class=\"column-3\">635<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-27\">\n\t<td class=\"column-1\">PE-Texas Red<\/td><td class=\"column-2\">610\/20<\/td><td class=\"column-3\">600<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-28\">\n\t<td class=\"column-1\">PE<\/td><td class=\"column-2\">586\/15<\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-29\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-30\">\n\t<td class=\"column-1\"><h5>Red Laser 640<\/h5><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-31\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-32\">\n\t<td class=\"column-1\">APC-Cy7<\/td><td class=\"column-2\">780\/60<\/td><td class=\"column-3\">750<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-33\">\n\t<td class=\"column-1\">Alexa Fluor 700<\/td><td class=\"column-2\">730\/45<\/td><td class=\"column-3\">690<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-34\">\n\t<td class=\"column-1\">APC<\/td><td class=\"column-2\">670\/14<\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-35\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-36\">\n\t<td class=\"column-1\"><h5>Available filters<\/h5><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-37\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-38\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\">685\/35<\/td><td class=\"column-3\">670<\/td><td class=\"column-4\">1.5 in use Aria<\/td>\n<\/tr>\n<tr class=\"row-39\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\">675\/50<\/td><td class=\"column-3\">635<\/td><td class=\"column-4\">1.0 in use Fortessa<\/td>\n<\/tr>\n<tr class=\"row-40\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\">670\/30<\/td><td class=\"column-3\">595<\/td><td class=\"column-4\">2.0<\/td>\n<\/tr>\n<tr class=\"row-41\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\">660\/20<\/td><td class=\"column-3\">545<\/td><td class=\"column-4\">2.0<\/td>\n<\/tr>\n<tr class=\"row-42\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\">585\/42<\/td><td class=\"column-3\">535<\/td><td class=\"column-4\">1.5<\/td>\n<\/tr>\n<tr class=\"row-43\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\">560\/20<\/td><td class=\"column-3\"><\/td><td class=\"column-4\">1<\/td>\n<\/tr>\n<tr class=\"row-44\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\">560\/40<\/td><td class=\"column-3\"><\/td><td class=\"column-4\">1<\/td>\n<\/tr>\n<tr class=\"row-45\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\">540\/30<\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-46\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\">424\/44<\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Fluidics - Sample Flow Rates<\/h5><\/td><td class=\"column-2\"><ul><li>Front key panel provides three modes<\/li><ul style=\"list-style-type:circle;\"><li>RUN<\/li><li>STANDBY<\/li><li>PRIME<\/li><\/ul><br \/>\n<li>Continuously adjustable flow rates, plus three preset flow rates<\/li><ul style=\"list-style-type:circle;\"><li>LO  12 \u00b5L\/min<\/li><li>MED 35 \u00b5L\/min<\/li><li>HI 60 \u00b5L\/min<\/li><\/ul><\/ul><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Fluorescence sensitivity<\/h5><\/td><td class=\"column-2\"><ul><li>FITC : 80 molecules of equivalent soluble fluorochrome (MESF-FITC)<\/li><li>PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE)<\/li><li>PE-Cy\u21225: 10 molecules of equivalent soluble fluorochrome (MESF-PE-Cy5)<\/li><li>APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC)<\/li><\/ul><br \/>\nFITC and PE measurements performed using SPHERO\u2122 Rainbow Calibration Particles (RCP-30-5A) PE-Cy5 and APC measurements performed using SPHERO Ultra Rainbow Calibration Particles (URCP-38-2K)<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Fluorescence resolution<\/h5><\/td><td class=\"column-2\">Coefficient of variation (CV) PI: Area, <3.0%, full G0\/G1 peak for PI-stained chicken erythrocyte nuclei (CEN)<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Fluorescence linearity<\/h5><\/td><td class=\"column-2\">Doublet\/singlet ratio CEN stained with PI: 1.95\u20132.05 (488-nm laser)<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Forward and Side Scatter Sensitivity<\/h5><\/td><td class=\"column-2\">Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-\u00b5m beads<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Forward and Side Scatter Resolution<\/h5><\/td><td class=\"column-2\">Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><h5>Signal Processing - <br \/>\nConverter <\/h5><\/td><td class=\"column-2\">10-MHz analog-to-digital converter<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><h5>Signal Processing - <br \/>\nWorkstation Resolution<\/h5><\/td><td class=\"column-2\">262,144 channels<\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\"><h5>Signal Processing - <br \/>\nPulse Processing<\/h5><\/td><td class=\"column-2\">Height, area, and width measurements available for any parameter. Ratio measurements are also available<\/td>\n<\/tr>\n<tr class=\"row-12\">\n\t<td class=\"column-1\"><h5>Signal Processing - <br \/>\nTime<\/h5><\/td><td class=\"column-2\">Can be correlated to any parameter for kinetic experiments or other applications<\/td>\n<\/tr>\n<tr class=\"row-13\">\n\t<td class=\"column-1\"><h5>BD\u2122 High Throughput Sampler (HTS)<\/h5><\/td><td class=\"column-2\">Is available to increase your lab productivity by acquiring samples from a 96- or 384-well microtiter plate.<br \/>\n<br \/>\n<b>HTS throughput<\/b><br \/>\n<br \/>\n<u>Acquisition<\/u><br \/>\n<ul><li> less than 15 minutes per microtiter plate in high throughput mode using a 2-second acquisition<\/li><li>less than 44 minutes in standard mode using a 10-second acquisition<\/li><\/ul><br \/>\n<u>Carryover<\/u><br \/>\n<ul><li><0.5% HT mode<\/li><li><0.75% STD mode<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-56 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-91b0a7dd-f20e-4383-95bd-4b4f44c07af3\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-91b0a7dd-f20e-4383-95bd-4b4f44c07af3\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-1-3b2ca8d7-7f0e-4f1d-8a92-b91697ca1b6e\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-3b2ca8d7-7f0e-4f1d-8a92-b91697ca1b6e\" style=\"color: #000000; \"><strong>BD FACS Aria Fusion<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-1-3b2ca8d7-7f0e-4f1d-8a92-b91697ca1b6e\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"265\" height=\"198\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/bd-facsaria-fusion-regular280.jpg\" alt=\"\" class=\"wp-image-425\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Fluorescence Activated Cell Sorting<\/li>\n\n\n\n<li>4 spatially separated lasers<\/li>\n\n\n\n<li>16 fluorescence detectors<\/li>\n\n\n\n<li>T\u00b0 control of a sample\n<ul class=\"wp-block-list\">\n<li>Sorting on\/into device options:<ul style=\"list-style-type:circle;\"><li>1.5mL tubes<\/li><li>5ml-, 15ml- tubes<\/li><li>6-, 24-, 48-, 96-, 384-well plates<\/li><li>Microscope slides<\/li><\/ul><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p><strong>Description<\/strong><\/p>\n\n\n\n<p>Fluorescence activated cell sorter (FACS) with a quartz cuvette flow cell that is in fixed alignment with the laser, and is gel coupled to the collection optics. BD FACSAriaTM Fusion is equipped with 4 spatially separated lasers, 16 fluorescent detectors and 2 light scatter detectors for fast multicolour analysis and cell sorting with accurate event data acquisition at up to 70,000 events per second. BD FACSAriaTM Fusion is placed in the biosafety cabinet (BSC) Class II Type A2, designed in collaboration with The Baker Company, a leader in biosafety solutions.<\/p>\n\n\n\n<p>Link to the manufacturer: https:\/\/www.bdbiosciences.com\/en-us<\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<p>Flow cytometry analysis and fluorescence activated cell sorting.<\/p>\n\n\n\n<p><strong>Specification<\/strong><\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-9d5b5b56-54a1-4ab4-9bf1-b8f0d37e768f\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-9d5b5b56-54a1-4ab4-9bf1-b8f0d37e768f\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-9d5b5b56-54a1-4ab4-9bf1-b8f0d37e768f\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-9d5b5b56-54a1-4ab4-9bf1-b8f0d37e768f\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-9d5b5b56-54a1-4ab4-9bf1-b8f0d37e768f\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-59\" class=\"tablepress tablepress-id-59\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Excitation Optics<\/h5><\/td><td class=\"column-2\">Fixed optical alignment of all Class IIIb lasers with the cuvette flow cell. All lasers are solid state and elliptical <ul><li>Beam height: 9 \u00b13 \u00b5m<\/li><li>Beam width: 65 \u00b17 \u00b5m<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Emission Optics<\/h5><\/td><td class=\"column-2\">Forward Scatter Detector: photodiode with 488\/10 bandpass filter for the 488-nm laser<br \/>\nSide Scatter Detector: photomultipler with a 488\/10 bandpass filter for the 488-nm laser<br \/>\n<br \/>\nFluorescence Detectors and Filters:<br \/>\n\n<table id=\"tablepress-60\" class=\"tablepress tablepress-id-60\">\n<thead>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><\/td><th class=\"column-2\">BP<\/th><th class=\"column-3\">LP<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Violet Laser 405<\/h5><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">BV786<\/td><td class=\"column-2\">780\/60<\/td><td class=\"column-3\">750<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">BV711<\/td><td class=\"column-2\">710\/50<\/td><td class=\"column-3\">690<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">Qdot605<\/td><td class=\"column-2\">610\/20<\/td><td class=\"column-3\">595<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\">Qdot585<\/td><td class=\"column-2\">586\/20<\/td><td class=\"column-3\">550<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\">BV510<\/td><td class=\"column-2\">510\/30<\/td><td class=\"column-3\">495<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\">DAPI<\/td><td class=\"column-2\">450\/50<\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\"><h5>Blue Laser 488<\/h5><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-12\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-13\">\n\t<td class=\"column-1\">PerCP-Cy5.5<\/td><td class=\"column-2\">695\/40<\/td><td class=\"column-3\">655<\/td>\n<\/tr>\n<tr class=\"row-14\">\n\t<td class=\"column-1\">FITC<\/td><td class=\"column-2\">525\/20<\/td><td class=\"column-3\">505<\/td>\n<\/tr>\n<tr class=\"row-15\">\n\t<td class=\"column-1\">SSC<\/td><td class=\"column-2\">488\/10<\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-16\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-17\">\n\t<td class=\"column-1\"><h5>YellGreen Laser 561<\/h5><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-18\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-19\">\n\t<td class=\"column-1\">PE-Cy7<\/td><td class=\"column-2\">780\/60<\/td><td class=\"column-3\">750<\/td>\n<\/tr>\n<tr class=\"row-20\">\n\t<td class=\"column-1\">PE-Cy5<\/td><td class=\"column-2\">690\/20<\/td><td class=\"column-3\">670<\/td>\n<\/tr>\n<tr class=\"row-21\">\n\t<td class=\"column-1\">PE-Cy5.5<\/td><td class=\"column-2\">670\/14<\/td><td class=\"column-3\">630<\/td>\n<\/tr>\n<tr class=\"row-22\">\n\t<td class=\"column-1\">PE-Texas Red<\/td><td class=\"column-2\">610\/20<\/td><td class=\"column-3\">600<\/td>\n<\/tr>\n<tr class=\"row-23\">\n\t<td class=\"column-1\">PE<\/td><td class=\"column-2\">586\/15<\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-24\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-25\">\n\t<td class=\"column-1\"><h5>Red Laser 640<\/h5><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-26\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<tr class=\"row-27\">\n\t<td class=\"column-1\">APC-Cy7<\/td><td class=\"column-2\">780\/60<\/td><td class=\"column-3\">735<\/td>\n<\/tr>\n<tr class=\"row-28\">\n\t<td class=\"column-1\">Alexa Fluor 700<\/td><td class=\"column-2\">705\/20<\/td><td class=\"column-3\">685<\/td>\n<\/tr>\n<tr class=\"row-29\">\n\t<td class=\"column-1\">APC<\/td><td class=\"column-2\">670\/30<\/td><td class=\"column-3\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Fluidics<\/h5><\/td><td class=\"column-2\"><ul><li>Sheath pressure is adjustable from 5 to 75 psi<\/li><li>Sample Flow Rates adjustable dynamic range<\/li><li>Nozzles are removable and can be sonicated; available in 3 sizes: 70, 85 and <br \/>\n     100-\u00b5m<\/li><li>Temperature control of sample input: 4\u00baC, 20\u00baC, 37\u00baC, and 42\u00baC (adjusted in the software); sample output: from +5\u00b0C to +42\u00b0C<\/li><li>Collection devices: 15ml- and 5ml-tubes, eppendorfs, 6, 24, 96, and 384-well plates. Index sorting can be enabled when sorting single cells. This capability indexes the cell surface phenotype to the well containing that cell<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Fluorescence sensitivity<\/h5><\/td><td class=\"column-2\">Measurements performed at 70 psi and 90 kHz using SPHERO\u2122 Rainbow Calibration Particles (RCP-30-5A).*<br \/>\n*MESF, can vary lot-to-lot<br \/>\n<br \/>\n<ul><li>FITC : 87 molecules of equivalent soluble fluorochrome (MESF-FITC)<\/li><li>PE: 29 molecules of equivalent soluble fluorochrome (MESF-PE)<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Fluorescence resolution<\/h5><\/td><td class=\"column-2\">Coefficient of variation (CV)<br \/>\n<br \/>\n<ul><li>PI: Area, <3.0%, full G0\/G1 peak for PI-stained chicken erythrocyte nuclei (CEN)<\/li><li>Hoechst: Area, <3.5%, full G0\/G1 peak for Hoechst-stained chicken erythrocyte nuclei (CEN)<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Fluorescence linearity<\/h5><\/td><td class=\"column-2\">Doublet\/singlet ratio CEN stained with PI: 1.95\u20132.05 (488-nm laser) or Hoechst: 1.95\u20132.05 (405-nm laser)<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Forward and Side Scatter Sensitivity<\/h5><\/td><td class=\"column-2\">Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-\u00b5m beads<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Forward and Side Scatter Resolution<\/h5><\/td><td class=\"column-2\">Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><h5>Soft Performance - Drop Drive Frequency<\/h5><\/td><td class=\"column-2\">Range: 1\u2013100,000 Hz<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><h5>Soft Performance - Purity and Yield<\/h5><\/td><td class=\"column-2\">At 70 psi and 87 kHz with an average threshold rate of 25,000 events per second, a four-way sort achieved a purity of >98% and a yield >80% of Poisson\u2019s expected yield. Higher threshold rates up to 70,000 events per second can be achieved without affecting purity. However, yield will decrease based on Poisson\u2019s statistics<\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\"><h5>Soft Performance - Functionality<\/h5><\/td><td class=\"column-2\">Dendritic cells (myeloid and plasmacytoid, mDs and pDCs, respectively) were isolated from the peripheral blood mononuclear cells of three donors and sorted on the BD FACSAria III system (one sort per donor) which uses the same cuvettebased flow cell design as the BD FACSAria Fusion. Post-sort cell viability was assessed using a<br \/>\nlive\/dead exclusion marker, and functionality was assessed by intracellular cytokine staining after 6 or 18 hours of stimulation with the TLR 7 &amp; 8 agonist R848. Post-sort viability at 6\u201318 hours was >90% for all three donors. Both pDCs and mDCs for all three donors produced IFN-a, TNF-a, and IL-12 stimulation, demonstrating post-sort functionality<\/td>\n<\/tr>\n<tr class=\"row-12\">\n\t<td class=\"column-1\"><h5>Soft Performance - Sort Collection Devices<\/h5><\/td><td class=\"column-2\"><ul><li>Two-way sorting: 12 x 75-mm and 15-mL tubes<\/li><li>Four-way sorting: 1.5-mL microtubes and 12 x 75-mm tubes <\/li><li>Single cell sort into multiwall plates: 6-; 24-; 96-; 384-well plates<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-13\">\n\t<td class=\"column-1\"><h5>Soft Performance - FACS\u2122 Accudrop<\/h5><\/td><td class=\"column-2\"><ul><li>Red diode laser provided for fully automated drop-delay determination<\/li><li>Automated drop breakoff monitoring <\/li><li>Automated clog detection and sort tube protection system using Sweet Spot technology<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-14\">\n\t<td class=\"column-1\"><h5>Signal Processing - <br \/>\nConverter <\/h5><\/td><td class=\"column-2\">10-MHz analog-to-digital converter. Pulse sampling is precisely matched to the particle flow rate in the cuvette. Particles travel slower compared to conventional stream-in-air sorters. This increases the light collected, resulting in better sensitivity. High-speed sorting is achieved by accelerating the stream through the nozzle, achieving drop rates comparable to stream-in-air sorters. The flow cell design and electronics are matched to maximize signal while maintaining maximum sort speed, purity, and yield<\/td>\n<\/tr>\n<tr class=\"row-15\">\n\t<td class=\"column-1\"><h5>Signal Processing - <br \/>\nWorkstation Resolution<\/h5><\/td><td class=\"column-2\">262,144 channels<\/td>\n<\/tr>\n<tr class=\"row-16\">\n\t<td class=\"column-1\"><h5>Signal Processing - <br \/>\nPulse Processing<\/h5><\/td><td class=\"column-2\">Height, area, and width measurements available for any parameter. Ratio measurements are also available<\/td>\n<\/tr>\n<tr class=\"row-17\">\n\t<td class=\"column-1\"><h5>Signal Processing - <br \/>\nTime<\/h5><\/td><td class=\"column-2\">Can be correlated to any parameter for kinetic experiments or other applications<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-59 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-9d5b5b56-54a1-4ab4-9bf1-b8f0d37e768f\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-9d5b5b56-54a1-4ab4-9bf1-b8f0d37e768f\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-2-3b2ca8d7-7f0e-4f1d-8a92-b91697ca1b6e\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-3b2ca8d7-7f0e-4f1d-8a92-b91697ca1b6e\" style=\"color: #000000; \"><strong>Cytek\u00ae Aurora<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-2-3b2ca8d7-7f0e-4f1d-8a92-b91697ca1b6e\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"225\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2023\/01\/aurora.jpg\" alt=\"\" class=\"wp-image-1926\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Benchtop spectral flow cytometer<\/li>\n\n\n\n<li>5 spatially separated lasers<\/li>\n\n\n\n<li>64 fluorescent detectors\n<ul class=\"wp-block-list\">\n<li>Sample loading options:<ul style=\"list-style-type:circle;\"><li>5ml tubes<\/li><li>96 &#8211; well plates<\/li><\/ul><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p><strong>Description<\/strong><\/p>\n\n\n\n<p>The Cytek Aurora cytometer is a benchtop spectral flow cytometer. Equipped with 5 spatially separated lasers, 64 fluorescent detectors and 3 light scatter detectors for fast multicolour analysis with accurate data acquisition rate at up to 30,000 events per second (with beads). The Cytek Aurora cytometer is equipped with plate loader system for acquiring samples from a 96 well plates. Plate loader has controlled plate stage temperature within the range 4-30\u00b0 C.<\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<p>Multicolour flow cytometry analysis, high autofluorescence and background substraction, high speed data aquisition.<\/p>\n\n\n\n<p><strong>Specification<\/strong><\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-f609a599-7325-4990-9209-23ea5323a155\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-f609a599-7325-4990-9209-23ea5323a155\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-f609a599-7325-4990-9209-23ea5323a155\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-f609a599-7325-4990-9209-23ea5323a155\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-f609a599-7325-4990-9209-23ea5323a155\" aria-hidden=\"true\">\n\t\t\t\n\n<p><a rel=\"noreferrer noopener\" href=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2021\/12\/Aurora_detector_config.pdf\" target=\"_blank\">Detector configuration [PDF]<\/a><\/p>\n\n\n\n<table id=\"tablepress-61\" class=\"tablepress tablepress-id-61\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Excitation Optics<\/h5><\/td><td class=\"column-2\">Laser power<br \/>\n<br \/>\n<ul><li>355 nm: 20 mW<\/li><li>405 nm: 100 mW<\/li><li>488 nm: 50 mW<\/li><li>561 nm: 50 mW<\/li><li>640 nm: 80 mW<\/li><\/ul><br \/>\nFlat Top Laser beam profile with narrow vertical beam height optimized for small particle detection.<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Emission Optics<\/h5><\/td><td class=\"column-2\">Forward Scatter Detector: High-performance semiconductor detector with 488 bandpass filter<br \/>\nSide Scatter Detector: Two high-performance semiconductor detector with 405 and 488 bandpass filters<br \/>\n<br \/>\nFluorescence Detectors and Filters:<br \/>\nThe Cytek Aurora cytometer is equipped with Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) 8-16 - channel semiconductor detector array per laser enabling more efficient spectrum capture for dyes emitting in the 400-900 nm range. No filter changes required for any fluorochrome excited by the 355nm, 405nm, 488nm, 561nm and 640nm lasers.<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Fluidics<\/h5><\/td><td class=\"column-2\"><ul><li>Sample Flow Rates - Low: 20 \u03bcL\/min, Medium: 40 \u03bcL\/min, High: 80 \u03bcL\/min<\/li><li>Sample input format: Manual (12x75mm polystyrene and polypropylene tubes) and automated (96 well plate) sample input formats<\/li><li>Sample dead volume: 5\u03bcl for 12x75mm tube<\/li><li>Sample restoration mode:  only for 96 well plate loader<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Fluorescence sensitivity<\/h5><\/td><td class=\"column-2\"><ul><li>FITC : <110 MEFL<\/li><li>PE: <35 MEFL<\/li><li>APC: <15 MEFL<\/li><li>Pacific Blue: <190 MEFL<\/li><\/ul><br \/>\n*measurements based on an average from three systems and performed using SPHERO Rainbow<br \/>\nCalibration Particle (RCP 30-5A) based on its peak emission channel<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Fluorescence linearity<\/h5><\/td><td class=\"column-2\">FITC R2 \u22650.995 \/ PE R2 \u22650.995<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Forward and Side Scatter Sensitivity<\/h5><\/td><td class=\"column-2\">Enables separation of fixed platelets from noise<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Forward and Side Scatter Resolution<\/h5><\/td><td class=\"column-2\">Performance is optimized for resolving lymphocytes, monocytes, and granulocytes<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Side Scatter Resolution<\/h5><\/td><td class=\"column-2\">Capable of resolving 0.2\u03bcm beads from noise<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><h5>Carryover<\/h5><\/td><td class=\"column-2\"> <0.1%<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><h5>Data Acquisition Rate<\/h5><\/td><td class=\"column-2\">35,000 events\/s<\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\"><h5>Spectroflo Software<\/h5><\/td><td class=\"column-2\"><ul><li>Live unmixing during acquisition<\/li><li> Developed specifically to streamline assay setup, data acquisition, and file export <\/li><li>Automated QC module<\/li><li>Auto-fluorescence extraction<\/li><li>Raw and Unmixed FCS 3.1 files<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-12\">\n\t<td class=\"column-1\"><h5>Signal Processing <\/h5><\/td><td class=\"column-2\">Digital signal processing with automatic window gate adjustment. 22-bit 6.5 log decades. Threshold using any single parameter or combination of parameters.<\/td>\n<\/tr>\n<tr class=\"row-13\">\n\t<td class=\"column-1\"><h5> Pulse Shape Parameters<\/h5><\/td><td class=\"column-2\">Pulse Area and Height for every parameter. Width for scatter parameters and one fluorescence parameter for each laser.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-61 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-f609a599-7325-4990-9209-23ea5323a155\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-f609a599-7325-4990-9209-23ea5323a155\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"sidebar-page.php","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-1846","page","type-page","status-publish","hentry"],"featured_image_src":null,"_links":{"self":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/1846","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/comments?post=1846"}],"version-history":[{"count":42,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/1846\/revisions"}],"predecessor-version":[{"id":2037,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/1846\/revisions\/2037"}],"wp:attachment":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/media?parent=1846"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}