{"id":1647,"date":"2023-02-08T14:44:49","date_gmt":"2023-02-08T14:44:49","guid":{"rendered":"http:\/\/imcf.natur.cuni.cz\/IMCF\/?page_id=1647"},"modified":"2026-03-12T07:55:45","modified_gmt":"2026-03-12T07:55:45","slug":"electron-microscopes","status":"publish","type":"page","link":"https:\/\/imcf.natur.cuni.cz\/IMCF\/electron-microscopes\/","title":{"rendered":"Electron microscopes and sample preparation equipment"},"content":{"rendered":"\n<p style=\"font-size:26px\"><strong>Electron microscopes<\/strong><\/p>\n\n\n<div class=\"wp-block-ub-content-toggle wp-block-ub-content-toggle-block\" id=\"ub-content-toggle-block-372b612e-73cc-49e3-a172-87aff2ded326\" data-mobilecollapse=\"false\" data-desktopcollapse=\"true\" data-preventcollapse=\"false\" data-showonlyone=\"true\">\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-0-372b612e-73cc-49e3-a172-87aff2ded326\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-372b612e-73cc-49e3-a172-87aff2ded326\" style=\"color: #000000; \"><strong>FEI Helios NanoLab 660 G3 UC<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-0-372b612e-73cc-49e3-a172-87aff2ded326\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"210\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/fei_helios_1-regular280.jpg\" alt=\"\" class=\"wp-image-321\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>FIB-SEM DualBeam microscope<\/li>\n\n\n\n<li>High resolution scanning electron microscopy with focus ion beam<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Scanning electron microscope with focused ion beam milling equipped for cryo-imaging and correlative light and electron microscopy (CLEM)<\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>3D scanning electron microscopy (FIB-SEM)<\/li>\n\n\n\n<li>high resolution scanning electron microscopy (embedded, dried or frozen samples)<\/li>\n\n\n\n<li>elemental analysis (EDS)<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-25d58fa5-b9e8-4e68-9d4f-76d6662b46af\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-25d58fa5-b9e8-4e68-9d4f-76d6662b46af\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-25d58fa5-b9e8-4e68-9d4f-76d6662b46af\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-25d58fa5-b9e8-4e68-9d4f-76d6662b46af\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-25d58fa5-b9e8-4e68-9d4f-76d6662b46af\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-40\" class=\"tablepress tablepress-id-40\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">AutoSlice&amp;View 4.1, Amira 6 softwares for image analysis<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Electron and Ion sources<\/h5><\/td><td class=\"column-2\">Electron gun with Schottky thermal field emitter LMIS Ga3+ ion source<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Electron column\/optics<br \/>\nspecifications<\/h5><\/td><td class=\"column-2\"><ul><li>Available accelerating voltage: <b>350 V \u2013 30 kV<\/b><\/li><li>Probe current: <b>0,8 pA \u2013 100 nA<\/b><\/li><li>Imaging modes: <b>field free, XHR immersion, EDS optimized<\/b><\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Ion column\/optics<br \/>\nspecifications<\/h5><\/td><td class=\"column-2\"><ul><li>Ion column type: <b>Tomahawk<\/b><\/li><li>Available accelerating voltage: <b>500 V \u2013 30 kV<\/b><\/li><li>Probe current: <b>0,1 pA \u2013 65 nA<\/b><\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Detectors available<\/h5><\/td><td class=\"column-2\"><ul><li>In-lens detector (TLD \u2013 SE, BSE)<\/li><li>In-column SE detector (ICD)<\/li><li>In-column BSE detector (MD)<\/li><li>Everhart-Thornley SE detector (ETD)<\/li><li>Retractable high contrast solid-state backscatter electron<br \/>\ndetector (CBS)<\/li><li>Retractable STEM detector with BF\/DF\/HAADF segments<\/li><li>Secondary electrons\/ions detector (ICE)<\/li><li>Energy dispersive spectrometer (EDS)<\/li><li>Others: IR camera for viewing sample\/column, Chamber<br \/>\nmounted Nav-Cam+, Integrated beam current measurement<\/li><br \/>\n<\/ul><\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Resolution<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-41\" class=\"tablepress tablepress-id-41\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Top Resolution<\/th><th class=\"column-2\">Conditions<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">0,6 nm<\/td><td class=\"column-2\">30 kV (STEM)<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">0,6 nm<\/td><td class=\"column-2\">15 \u2013 2 kV<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">0,7 nm<\/td><td class=\"column-2\">1 kV<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">1,0 nm<\/td><td class=\"column-2\">500 V (ICD)<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">4,0 nm<\/td><td class=\"column-2\">30 kV (i-beam)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Stage<\/h5><\/td><td class=\"column-2\">High precision 5-axes 16 inch piezo motorized stage Cryo-stage Leica VCT100<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Others<\/h5><\/td><td class=\"column-2\">Gas Injection System \u2013 platinum and carbon depostion, Plasma cleaner<br \/>\n(mounted on the chamber), Micromanipulator EasyLift EX (FEI)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-25d58fa5-b9e8-4e68-9d4f-76d6662b46af\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-25d58fa5-b9e8-4e68-9d4f-76d6662b46af\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-1-372b612e-73cc-49e3-a172-87aff2ded326\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-372b612e-73cc-49e3-a172-87aff2ded326\" style=\"color: #000000; \"><strong>JEOL JEM 2100-Plus 200kV<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-1-372b612e-73cc-49e3-a172-87aff2ded326\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"354\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/tem_photo-regular280.jpg\" alt=\"\" class=\"wp-image-331\" srcset=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/tem_photo-regular280.jpg 280w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/tem_photo-regular280-237x300.jpg 237w\" sizes=\"auto, (max-width: 280px) 100vw, 280px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Transmission Electron Microscope equipped with cryo-pole piece and on axis TVIPS TemCam\u2013XF416 4K CMOS camera with frame rate up to 24 f\/s and high sensitivity<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Transmission Electron Microscope equipped with a cryo-pole piece, fast and sensitive TVIPS XF-416 4kx4k camera and side-entry cryo holder Gatan 626.<\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Transmission electron microscopy (TEM) at room temperature<\/li>\n\n\n\n<li>Advanced sample mapping<\/li>\n\n\n\n<li>Electron tomography (ET)<\/li>\n\n\n\n<li>Diffraction at cryo and room temperature<\/li>\n\n\n\n<li>Cryo-electron microscopy<\/li>\n\n\n\n<li>SerialEM software for automated image acquisition, montage mapping, tomography in ambient and cryo conditions<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-f0395d6a-5678-43fb-b345-ae822a2604cc\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-f0395d6a-5678-43fb-b345-ae822a2604cc\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-f0395d6a-5678-43fb-b345-ae822a2604cc\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-f0395d6a-5678-43fb-b345-ae822a2604cc\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-f0395d6a-5678-43fb-b345-ae822a2604cc\" aria-hidden=\"true\">\n\t\t\t\n\n<p>JEOL manufacturer specifications (https:\/\/www.jeol.co.jp\/en\/products\/detail\/JEM-2100.html)<\/p>\n\n\n\n<table id=\"tablepress-42\" class=\"tablepress tablepress-id-42\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">TEM Center, EM Menu 5, SerialEM<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Electron sources<\/h5><\/td><td class=\"column-2\">Single crystal LaB6 cathode with a current range of at least 4pA to 40nA<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Acceleration voltage (min. step)<\/h5><\/td><td class=\"column-2\">80kV - 200kV (50 V)<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Stability<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-44\" class=\"tablepress tablepress-id-44\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Acc. Voltage<\/th><th class=\"column-2\">OL Current<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">2 x 10-6 \/min<\/td><td class=\"column-2\">1 x 10-6 \/min<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Magnification range<br \/>\n(MAG\/Low MAG mode)<\/h5><\/td><td class=\"column-2\">50x \u2013 1.000.000x<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Bottom Mount High-sensitivity<br \/>\nCMOS Camera<\/h5><\/td><td class=\"column-2\"><ul><li>TVIPS TemCam\u2013XF416<\/li><li>4k x 4k (4096 x 4096 pxl)<\/li><li>Signal\/noise ratio 15:1 (200 kV)<\/li><li>Frame rate up to 24 f\/s<\/li><li>Physical pixel size 15.5 um<\/li><li>Field of view 63.5 um<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Camera length<\/h5><\/td><td class=\"column-2\">SA DIFF (mm) : 100 to 2,500<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Resolution at 200 kV<\/h5><\/td><td class=\"column-2\">Point resolution 0.27 nm\/Line resolution 0.14 nm<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><h5>Optical paremeters for<br \/>\nobjective lenses<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-43\" class=\"tablepress tablepress-id-43\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Focal Length <\/th><th class=\"column-2\">Spherical aber.coeff.<\/th><th class=\"column-3\">Chromatic aber.coeff.<\/th><th class=\"column-4\">Minimal focal step<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">2.8 mm<\/td><td class=\"column-2\">2.0 mm<\/td><td class=\"column-3\">2.1 mm<\/td><td class=\"column-4\">2.0 mm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><h5>Goniometer tilt<\/h5><\/td><td class=\"column-2\">From 70\u00b0 to - 70\u00b0<\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\"><h5>Holders<\/h5><\/td><td class=\"column-2\"><ul><li>Single tilt holder (1 grid)<\/li><li>Specimen quartet holder (4 grids)<\/li><li>High-tilt holder (1 grid)<\/li><li>Cryo-holder Gatan 626<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-12\">\n\t<td class=\"column-1\"><h5>Others<\/h5><\/td><td class=\"column-2\"><ul><li>Minimal Dose System (MDS)<\/li><li>Anti-contamination device (ACD)<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-f0395d6a-5678-43fb-b345-ae822a2604cc\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-f0395d6a-5678-43fb-b345-ae822a2604cc\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div><\/div>\n<\/div>\n\n<\/div>\n\t\t<\/div>\n<\/div>\n\n\n<p><\/p>\n\n\n\n<p style=\"font-size:26px\"><strong>Sample preparation equipment<\/strong><\/p>\n\n\n\n<p><\/p>\n\n\n<div class=\"wp-block-ub-content-toggle wp-block-ub-content-toggle-block\" id=\"ub-content-toggle-block-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" data-mobilecollapse=\"false\" data-desktopcollapse=\"true\" data-preventcollapse=\"false\" data-showonlyone=\"true\">\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-0-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" style=\"color: #000000; \"><strong>Automatic Plunge Freezer EM GP2<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-0-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"213\" height=\"300\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2022\/12\/AutPlFr.jpg\" alt=\"\" class=\"wp-image-1703\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>The EM GP2 plunge freezes fluid or extremely thin samples spread on an electron microscopy grid into liquid ethane after removing excess fluid by automatic blotting.<\/li>\n\n\n\n<li>Prior to freezing the sample is maintained in a temperature and humidity controlled environmental chamber, adjustable between +4\u00b0C and +60\u00b0C and room humidity to 99 %<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>Leica EM GP2 on Leica Microsystems webpage                              (https:\/\/www.leica-microsystems.com\/products\/sample-preparation-for-electron-microscopy\/p\/leica-em-gp2\/)<\/p>\n\n\n\n<p>Leica EM GP2 Flyer                                                                          (https:\/\/www.leica-microsystems.com\/products\/sample-preparation-for-electron-microscopy\/p\/leica-em-gp2\/)<\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-0c6e91d9-1997-4ec9-ab11-3dc20254b00c\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-d473247c-3d73-4ee7-8e5b-6dc45afb48e8\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-d473247c-3d73-4ee7-8e5b-6dc45afb48e8\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-d473247c-3d73-4ee7-8e5b-6dc45afb48e8\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-d473247c-3d73-4ee7-8e5b-6dc45afb48e8\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-45\" class=\"tablepress tablepress-id-45\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Operating Conditions<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-46\" class=\"tablepress tablepress-id-46\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Feature<\/th><th class=\"column-2\">Min\/Max<\/th><th class=\"column-3\">Ideal<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">Room Temperature<\/td><td class=\"column-2\">+15 to +30 \u00b0C<\/td><td class=\"column-3\">+21 to +24 \u00b0C<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">Room Rel. Humidity<\/td><td class=\"column-2\">Max. 55 %<\/td><td class=\"column-3\">Around 40 %<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">Storage Temperature<\/td><td class=\"column-2\">+5 to +35\u00b0C<\/td><td class=\"column-3\">+21 to +24 \u00b0C<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">Storage Humidity<\/td><td class=\"column-2\">Max. 55 %<\/td><td class=\"column-3\">Around 40 %<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<b>Room Ventilation is required at all times during operation.<\/b><\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Environmental Chamber<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-47\" class=\"tablepress tablepress-id-47\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Feature<\/th><th class=\"column-2\">Temperature Control<\/th><th class=\"column-3\">Humidity Control <\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">Min\/Max<\/td><td class=\"column-2\">+4 to +60 \u00b0C<\/td><td class=\"column-3\">Room Humidity to 99%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\nChamber Features: Light (LED), Anti-Fog Heating <\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Dewar<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-48\" class=\"tablepress tablepress-id-48\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">LN2 Dewar Volume<\/th><th class=\"column-2\">Dewar Temperature<\/th><th class=\"column-3\">Dewar bake-out Temperature<\/th><th class=\"column-4\">LN2 Consumption <\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">1 l<\/td><td class=\"column-2\">-196 \u00b0C<\/td><td class=\"column-3\">Max. +110\u00b0C<\/td><td class=\"column-4\">~ 1 l\/h1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Preparation Chamber<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-49\" class=\"tablepress tablepress-id-49\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Allowed Secondary Cryogen<\/th><th class=\"column-2\">Secondary Cryogen Container Temperature<\/th><th class=\"column-3\">Secondary Cryogen Container Volume <\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">Ethane \/ Propane<\/td><td class=\"column-2\">0 to -196 \u00b0C<\/td><td class=\"column-3\">2.5 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-d473247c-3d73-4ee7-8e5b-6dc45afb48e8\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-d473247c-3d73-4ee7-8e5b-6dc45afb48e8\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div><\/div>\n<\/div>\n\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-1-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" style=\"color: #000000; \"><strong>Leica EM ICE \u2013 high pressure freezing machine<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-1-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"302\" height=\"227\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/10\/IMG_70572-1.jpg\" alt=\"\" class=\"wp-image-2686\" srcset=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/10\/IMG_70572-1.jpg 302w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/10\/IMG_70572-1-300x225.jpg 300w\" sizes=\"auto, (max-width: 302px) 100vw, 302px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>cryofixation of samples for EM<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>High pressure freezing system for cryo-fixation (vitrification) of samples for EM. There are several advantages over chemical fixation. Sample is freeze in a range of milliseconds, which immobilize all macromolecular components simultaneously. This technique also improves preservation of biological samples.<\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-79bfde67-38be-44f7-a8a7-ab948248204d\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-67147424-dff9-4720-870f-ba60dc022c10\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-67147424-dff9-4720-870f-ba60dc022c10\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-67147424-dff9-4720-870f-ba60dc022c10\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-67147424-dff9-4720-870f-ba60dc022c10\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-53\" class=\"tablepress tablepress-id-53\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Types of sample<\/h5><\/td><td class=\"column-2\"><ul><li>Cell suspension<\/li><li>Cell monolayer<br \/>\n<\/li><li>Tissue<\/li><li>Hydrogel<\/li><\/ul><br \/>\n<b>Size limitation is to 6 mm carrier: height 200 \u00b5m, diameter 5 mm<\/b><\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Application<\/h5><\/td><td class=\"column-2\"><ul><li>Freeze fracturing<\/li><li>Cryofixation for SEM, TEM FIB-SEM<\/li><li>Sample preparation for correlative light and electron microscopy<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-67147424-dff9-4720-870f-ba60dc022c10\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-67147424-dff9-4720-870f-ba60dc022c10\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div><\/div>\n<\/div>\n\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-2-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" style=\"color: #000000; \"><strong>Leica EM ACE600 \u2013 coating system<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-2-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"373\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_coating1-regular280.jpg\" alt=\"\" class=\"wp-image-339\" style=\"width:178px;height:237px\" srcset=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_coating1-regular280.jpg 280w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_coating1-regular280-225x300.jpg 225w\" sizes=\"auto, (max-width: 280px) 100vw, 280px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>precise coating of samples foe subsequent examination with an electron microscope<\/li>\n\n\n\n<li>glow discharge<\/li>\n\n\n\n<li>freeze fracture cryo set<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p><strong>Application<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Metal coating of sample to cerate a conductive layer which inhibits charging, reduces thermal demage and improves the secondary electron signal in the SEM<\/li>\n\n\n\n<li>Making the carbon layers to be transparent to the electron beam but conductive  (needed for X-ray microanalysis), to support films on grids<\/li>\n\n\n\n<li>Glow discharge to render grids surface hydrophilic<\/li>\n\n\n\n<li>Freeze-Fracture of frozen samples<\/li>\n<\/ul>\n\n\n\n<p><strong>Main components<\/strong><\/p>\n\n\n\n<p>The Leica EM ACE600 includes the following main functional units:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>High vacuum chamber<\/li>\n\n\n\n<li>Rotation sample stage, 24 positions for 12,7 mm SEM stubs<\/li>\n\n\n\n<li>Quartz thickness measurement<\/li>\n\n\n\n<li>Carbon thread source or sputter source (platinum and gold targets)<\/li>\n\n\n\n<li>Freeze-Fracture cryo set includes vacuum cyro transfer Leica EM VCT100 and cryo stage<\/li>\n<\/ul>\n\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-3-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" style=\"color: #000000; \"><strong>Leica EM CPD300 \u2013 critical point dryer<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-3-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"374\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_critical_1-regular280.jpg\" alt=\"\" class=\"wp-image-341\" srcset=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_critical_1-regular280.jpg 280w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_critical_1-regular280-225x300.jpg 225w\" sizes=\"auto, (max-width: 280px) 100vw, 280px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>drying of samples with CO2 in critical point for SEM<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Automated Critical Point Dryer<\/p>\n\n\n\n<p><strong>Application<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>The drying of biological specimens such as pollen, tissue, plants, insects for SEM analysis<\/li>\n\n\n\n<li>The drying of industrial samples , for example MEMS (Micro Electro Mechanical Systems)<\/li>\n<\/ul>\n\n\n\n<p><strong>Method<\/strong><\/p>\n\n\n\n<p>Crytical point drying is a method which use of CO2 as transitional fluid because its critical point is at 31 \u00b0C and 73,8 bar. Technically these temperature and pressure requirements of the CO2 can be implemented relatively easily then for water (critical point is 374 \u00b0C and 228,5 bar). The water in the cell is replaced by an acetone which is very soluble with liquid CO2. This procedure is follow by substitution with liquid CO2 through serial dilution steps. The increasing the temperature and pressure will transfer the CO2 through its critical point into a subcritical state. By controlled depressurization and constant temperature is subcritical CO2 convert into its gaseous phase without crossing the phase boundary between liquid and gas. This technique of preparing sample for scanning electron microscope is more gentle and better preserve structure than air drying sample.<\/p>\n\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-4-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" style=\"color: #000000; \"><strong>Leica EM AFS2 &amp; FSP (freeze substitution processor)<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-4-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"186\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_em_fsp-regular280.png\" alt=\"\" class=\"wp-image-342\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>An automatic reagent handling system for AFS2<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Automatic freeze substitution (AFS) connects cryo-fixation (e. g. high pressure freezing) and resin embedding. It is a process of dehydration in low temperatures without crystal formation. In the beginning of freeze substitution, amorphic water contained in sample is dissolved by an organic solvent (e.g. acetone, ethanol), which also includes chemical fixatives. After dehydration temperature rises to point where is resin embedding possible. Freeze substitution processor (FSP) is an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for freeze substitution. It also provides UV lamp for photopolymerization of acrylic resins.<\/p>\n\n\n\n<p><strong>Application<\/strong><\/p>\n\n\n\n<p>Cryo-substitution and resin embedding<\/p>\n\n\n\n<table id=\"tablepress-54\" class=\"tablepress tablepress-id-54\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Types of sample<\/h5><\/td><td class=\"column-2\"><ul><li>Cell suspension<\/li><li>Cell monolayer<\/li><li>Tissue<\/li><li>Hydrogel<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Resins<\/h5><\/td><td class=\"column-2\"><ul><li>Epoxy resins: Embed 812, Araldite, Durcupan, Spurr, Ultrabed<\/li><li>Acrylic resins: Lowicryl, LR white<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-5-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" style=\"color: #000000; \"><strong>Leica EM AFS2 (automatic freeze substitution)<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-5-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"170\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_em_afs2-regular280.png\" alt=\"\" class=\"wp-image-343\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Cryo-substitution for samples frozen in HPM100<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Automatic freeze substitution (AFS) connects cryo-fixation (e. g. high pressure freezing) and resin embedding. It is a process of dehydration in low temperatures without crystal formation. In the beginning of freeze substitution, amorphic water contained in sample is dissolved by an organic solvent (e.g. acetone, ethanol), which also includes chemical fixatives. After dehydration temperature rises to point where is resin embedding possible. Freeze substitution processor (FSP) is an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for freeze substitution. It also provides UV lamp for photopolymerization of acrylic resins.<\/p>\n\n\n\n<p><strong>Application<\/strong><\/p>\n\n\n\n<p>Cryo-substitution and resin embedding<\/p>\n\n\n\n<table id=\"tablepress-54-no-2\" class=\"tablepress tablepress-id-54\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Types of sample<\/h5><\/td><td class=\"column-2\"><ul><li>Cell suspension<\/li><li>Cell monolayer<\/li><li>Tissue<\/li><li>Hydrogel<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Resins<\/h5><\/td><td class=\"column-2\"><ul><li>Epoxy resins: Embed 812, Araldite, Durcupan, Spurr, Ultrabed<\/li><li>Acrylic resins: Lowicryl, LR white<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-6-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" style=\"color: #000000; \"><strong>Pelco BioWavePro+ \u2013 microwave processor<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-6-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2022\/04\/Pelco_BioWave-1024x768.jpg\" alt=\"\" class=\"wp-image-1089\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>sample preparation for EM<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Microwave Processor containing Pelco ColdSpot\u00aePro cooling system<\/p>\n\n\n\n<p><strong>Application<\/strong><\/p>\n\n\n\n<p>Sample preparations for EM (fixation, contrastation, dehydratation, embedding, polymerisation)<\/p>\n\n\n\n<table id=\"tablepress-55\" class=\"tablepress tablepress-id-55\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Types of sample<\/h5><\/td><td class=\"column-2\"><ul><li>Histological tissues<\/li><li>Suspension cells<\/li><li>Hard-to infiltrate biological specimens<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Adjustable features<\/h5><\/td><td class=\"column-2\"><ul><li>Timing<\/li><li>Wattages (0 \u2013 750 W)<\/li><li>Temperature (0 \u2013 100\u00b0C)<br \/>\n<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>On\/Off systems<\/h5><\/td><td class=\"column-2\"><ul><li>Vacuum<\/li><li>Bubbler<\/li><li>Stirrer<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Other features<\/h5><\/td><td class=\"column-2\"><ul><li>Temperature check probe<\/li><li>Manual or Automatic running mode<\/li><li>Data recording<\/li><li>Protocols storage<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-7-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" style=\"color: #000000; \"><strong>Ultramicrotome Leica EM UC7<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-7-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"210\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2022\/07\/Microtome_A-scaled-1.jpg\" alt=\"\" class=\"wp-image-1171\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>EM UC7 Basic Instrument with eucentric 180\u00b0 swivelling microscope carrier<\/li>\n\n\n\n<li>Built in anti-vibration system<\/li>\n\n\n\n<li>Cutting window adjustment from 0.2-15 mm<\/li>\n\n\n\n<li>Cutting window adjustment from 0.2-15 mm<\/li>\n\n\n\n<li>Knife block 360\u00b0 rotatable, dual sided self locking precision drive with \u00b130\u00b0 graduation, clearance angle drive with 1\u00b0 scale from -2\u00b0 to over 15\u00b0<\/li>\n\n\n\n<li>Knife holder for 6-12mm glass and diamond knives<\/li>\n\n\n\n<li>360\u00b0 rotation of specimen with self locking drive<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p><strong>Specification<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Eucentric movement of the stereo microscope carrier (+ 5\u00b0 \/ &#8211; 8\u00b0) with patented designated positions for specimen approach, for glass and diamond knives<\/li>\n\n\n\n<li>Multi-LED-illumination for standard illumination, back light illumination, specimen<br>transillumination and spot light illumination<\/li>\n\n\n\n<li>Dual automatic advance system with stepping motor for 200\u03bcm specimen advance, stepping motor for 10mm knife N-S movement<\/li>\n\n\n\n<li>Stepping motor for 25mm E-W movement<\/li>\n\n\n\n<li>Reflexomat II -peristaltic water pump; water spout in magnetic holder<\/li>\n\n\n\n<li>Syringe, special tubing for Reflexomat II<\/li>\n\n\n\n<li>Segment arc with specimen transillumination, self locking precision drives with \u00b122\u00b0 eucentric movement, transferable stem for longitudinal and cross sectioning<\/li>\n<\/ul>\n\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-8-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\" style=\"color: #000000; \"><strong><strong>Stereomicroscope Leica Investa 3<\/strong> <strong>(Integrated camera)<\/strong><\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-8-8a80149a-39d2-4bd0-aa9f-382dc34f64bf\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:22%\">\n<figure class=\"wp-block-image size-medium\"><img loading=\"lazy\" decoding=\"async\" width=\"225\" height=\"300\" src=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2025\/11\/stereo-225x300.jpg\" alt=\"\" class=\"wp-image-3066\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Designed to record and facilitate sample dissection and micromanipulations, preparation and quality control of sample carriers a tools<\/li>\n\n\n\n<li>Optimized for stereomicroscopic inspection of samples, acquisition of documentation images\/movies, demonstrations and user training<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p><strong>Specification<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Integrated camera and output to an external monitor are suited for digital image sharing directly on different devices incl. external monitor, mobile device, or computer.<\/li>\n\n\n\n<li>Enables 3D view of the sample with an optimal 3D perception thanks to FusionOptics which combines high resolution and a large depth of field, large 9:1 zoom range, magnification of up to 55x and apochromatically corrected optics.<\/li>\n\n\n\n<li>Convenient to handle samples with the microscope\u2019s extra large working distance up to 122 mm.<\/li>\n\n\n\n<li>Enersight software interface can be used in multiple operation modes, such as on-screen display, mobile devices, or computer, allowing for inspection, comparative analysis and length\/angle measurements.<\/li>\n\n\n\n<li>Integrated camera can visualize sample movements on the monitor clearly and smoothly thanks to the high frame rate (up to 60 fps) with the fast auto exposure in the live image display.<\/li>\n\n\n\n<li>Leica Investa 3 Flyer (<a href=\"https:\/\/downloads.leica-microsystems.com\/Ivesta%203\/Brochure%20or%20Flyer\/Ivesta%203_Industry_brochure_EN.pdf\" data-type=\"link\" data-id=\"https:\/\/downloads.leica-microsystems.com\/Ivesta%203\/Brochure%20or%20Flyer\/Ivesta%203_Industry_brochure_EN.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">link<\/a>)<\/li>\n<\/ul>\n\n<\/div>\n\t\t<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Electron microscopes Sample preparation equipment<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"sidebar-page.php","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-1647","page","type-page","status-publish","hentry"],"featured_image_src":null,"_links":{"self":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/1647","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/comments?post=1647"}],"version-history":[{"count":76,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/1647\/revisions"}],"predecessor-version":[{"id":3174,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/1647\/revisions\/3174"}],"wp:attachment":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/media?parent=1647"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}