{"id":139,"date":"2020-06-04T14:16:32","date_gmt":"2020-06-04T14:16:32","guid":{"rendered":"http:\/\/web.natur.cuni.cz\/IMCF\/IMCF\/?page_id=139"},"modified":"2025-02-28T14:23:38","modified_gmt":"2025-02-28T14:23:38","slug":"electron-microscopy","status":"publish","type":"page","link":"https:\/\/imcf.natur.cuni.cz\/IMCF\/services\/electron-microscopy\/","title":{"rendered":"Electron microscopy"},"content":{"rendered":"\n<p>We cover the following methods:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Consultation on sample preparation, harvesting and controls <\/li>\n\n\n\n<li>Optimization of sample processing protocols and workflows<\/li>\n\n\n\n<li>Standard (chemical) sample preparation including Microwave processing<\/li>\n\n\n\n<li>Cryo-processing by High Pressure Freezing and Automated Freeze-Substitution<\/li>\n\n\n\n<li>Negative staining&nbsp;and Automated Plunge freezing followed by RT-TEM or cryo-TEM analysis<\/li>\n\n\n\n<li>TEM analysis of ultra-thin sections from resin-embedded cells, tissues and bio-composites<\/li>\n\n\n\n<li>3D-reconstruction of sub-cellular volumes by Electron Tomography<\/li>\n\n\n\n<li>Antigen detection and localization by immuno-gold TEM\/SEM<\/li>\n\n\n\n<li>Electron Diffraction of microcrystalline compounds<\/li>\n\n\n\n<li>Surface morphology of biological samples and materials by SEM<\/li>\n\n\n\n<li>Elemental analysis by EDS (in SEM)<\/li>\n\n\n\n<li>3D Ultrastructural analysis by FIB-SEM<\/li>\n\n\n\n<li>Correlative Light and Electron Microscopy (CLEM)<\/li>\n\n\n\n<li>EM data processing and quantitative analysis<\/li>\n\n\n\n<li>User training and support<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p>Samples we analyze:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Protein and macromolecular complexes<\/li>\n\n\n\n<li>Viruses, nanoparticles, liposomes<\/li>\n\n\n\n<li>Bacteria and yeast cells<\/li>\n\n\n\n<li>Adherent and suspension cells<\/li>\n\n\n\n<li>Tissues<\/li>\n\n\n\n<li>Organoids and small organisms<\/li>\n\n\n\n<li>Bio-composites and materials<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><a href=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/price-lists\/\" data-type=\"page\" data-id=\"2457\">Price list<\/a><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Acknowledgment<\/strong><\/h2>\n\n\n\n<p>When publishing data acquired using the facility\u2019s equipment or services, the users are required to acknowledge the facility. See <a href=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/acknowledgements\/\" target=\"_blank\" rel=\"noreferrer noopener\">here <\/a>for acknowledgement template and more information.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>We cover the following methods: Samples we analyze: Price list Acknowledgment When publishing data acquired using the facility\u2019s equipment or services, the users are required to acknowledge the facility. See here for acknowledgement template and more information.<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":135,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"sidebar-page.php","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-139","page","type-page","status-publish","hentry"],"featured_image_src":null,"_links":{"self":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/139","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/comments?post=139"}],"version-history":[{"count":26,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/139\/revisions"}],"predecessor-version":[{"id":2796,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/139\/revisions\/2796"}],"up":[{"embeddable":true,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/135"}],"wp:attachment":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/media?parent=139"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}