{"id":1353,"date":"2022-11-02T18:07:40","date_gmt":"2022-11-02T18:07:40","guid":{"rendered":"http:\/\/imcf.natur.cuni.cz\/IMCF\/?page_id=1353"},"modified":"2025-10-06T08:52:29","modified_gmt":"2025-10-06T08:52:29","slug":"optical-microscopes","status":"publish","type":"page","link":"https:\/\/imcf.natur.cuni.cz\/IMCF\/optical-microscopes\/","title":{"rendered":"Optical Microscopes"},"content":{"rendered":"<div class=\"wp-block-ub-content-toggle wp-block-ub-content-toggle-block\" id=\"ub-content-toggle-block-c7689ffa-8ae7-4152-b389-68571d10b70d\" data-mobilecollapse=\"false\" data-desktopcollapse=\"false\" data-preventcollapse=\"false\" data-showonlyone=\"true\">\n<div id=\"CZLSM880NLO\" class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-0-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Carl Zeiss LSM 880 NLO<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-0-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"265\" height=\"198\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/carl_zeiss_lsm-880_nlo-regular280.jpg\" alt=\"\" class=\"wp-image-359\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Inverted confocal microscope<\/li>\n\n\n\n<li>Multiphoton excitation and non-descanned detection with FLIM option<\/li>\n\n\n\n<li>Internal spectral detection unit<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Multi-functional fluorescence inverted confocal microscope with single- or multi-photon excitation, internal spectral detection and non-descanned detection with FLIM option.<\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Fast and multi-color confocal imaging<\/li>\n\n\n\n<li>Various measurement options: z-stack, time series, tile scan, multi positions and regions<\/li>\n\n\n\n<li>Spectral detection, spectral unmixing and fingerprinting<\/li>\n\n\n\n<li>Deep imaging of thick biological samples with multiphoton excitation and non-descanned detection<\/li>\n\n\n\n<li>Label free imaging: Coherent anti-Stokes Raman Scattering (CARS) and higher harmonic generation (SHG and THG)<\/li>\n\n\n\n<li>FLIM with multiphoton excitation<\/li>\n\n\n\n<li>Long term live-cell imaging available<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>Motorized inverted fluorescence microscope Carl Zeiss Axio Observer.Z1 with confocal module LSM 880 NLO equipped with the following:<\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-caab01d4-5b11-444d-80d0-d28a0ffebe67\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-caab01d4-5b11-444d-80d0-d28a0ffebe67\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-caab01d4-5b11-444d-80d0-d28a0ffebe67\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-caab01d4-5b11-444d-80d0-d28a0ffebe67\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-caab01d4-5b11-444d-80d0-d28a0ffebe67\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-30\" class=\"tablepress tablepress-id-30\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">ZEN 2.1 Black with following modules and functionalities:<br \/>\nSpectral Unmixing, 3D visualization, Tiles &amp; Positions, FRAP, Experiment designer<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Illumination<\/h5><\/td><td class=\"column-2\"><ul><li>Transmitted Light Illuminator HAL 100<\/li><li>Epifluorescence Illuminator HXP 120 V<\/li><li>Lasers (CW)<\/li><ul><li type=\"circle\">405 nm Laser 30 mW<\/li type=\"circle\"><li type=\"circle\">Argon Laser (458, 488, 514 nm) 25 mW<\/li type=\"circle\"><li type=\"circle\">561 nm Laser 20 mW<\/li type=\"circle\"><li type=\"circle\">633 nm Laser 5 mW<\/li type=\"circle\"><\/ul><li>Lasers (pulsed)<br \/>\n<ul><li type=\"circle\">Ti:Sapphire femtosecond laser Chameleon Ultra II (Coherent),<br \/>\n690-1080 nm,  > 3.5W<\/li type=\"circle\"><li type=\"circle\">Optical parametric oscillator (Chameleon Compact OPO, Coherent),<br \/>\n1100-1300 nm<\/li type=\"circle\"><li type=\"circle\">Delay line with LMS-270 precision liner stage (PI) in the Chameleon<br \/>\nbeam path<\/li type=\"circle\"><\/ul><\/ul><\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Detection<\/h5><\/td><td class=\"column-2\"><ul><li>2x transmitted light detector LSM T-PMT<\/li><li>Internal spectral detection unit<\/li><ul><li type=\"circle\">2 standard PMTs (\u201eblue\u201c and \u201ered\u201c 371-740 nm)<\/li type=\"circle\"><li type=\"circle\">Spectral detector 32-channels GaAsP PMT (410-695 nm)<\/li type=\"circle\"><li type=\"circle\">Spectral GaAsP detector and \u201ered\u201c PMT enables photon counting mode<\/li type=\"circle\"><\/ul><li>Non-descanned detection unit (reflected geometry)<\/li><ul><li type=\"circle\">Spectral detector 32BiG.2 unit (containing 2 detectors)<\/li type=\"circle\"><li type=\"circle\">High Speed Hybrid Detector for TCSPC HPM-100-40 (Becker &amp; Hickl), IRF 120 ps<\/li type=\"circle\"><li type=\"circle\">Range of dichroic beam-splitting filter cubes<\/li type=\"circle\"><\/ul><\/ul><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-31\" class=\"tablepress tablepress-id-31\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Objective Type<\/th><th class=\"column-2\">Immersion<\/th><th class=\"column-3\">Magnification<\/th><th class=\"column-4\">N.A.<\/th><th class=\"column-5\">WD[mm]<\/th><th class=\"column-6\">DIC\/Correct. Ring<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">EC Plan-Neofluar<br \/>\n420330-9901<\/td><td class=\"column-2\">Air<\/td><td class=\"column-3\">5x<\/td><td class=\"column-4\">0.16<\/td><td class=\"column-5\">18.5<\/td><td class=\"column-6\">-<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">Plan-Apochromat<br \/>\n420640-9900<\/td><td class=\"column-2\">Air<\/td><td class=\"column-3\">10x<\/td><td class=\"column-4\">0.45<\/td><td class=\"column-5\">2.10<\/td><td class=\"column-6\">-<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">LD LCI Plan-Apochromat<br \/>\n420852-9871<\/td><td class=\"column-2\">Oil, Water, Glycerine<\/td><td class=\"column-3\">25x<\/td><td class=\"column-4\">0.8<\/td><td class=\"column-5\">0.57*<\/td><td class=\"column-6\">DIC, Corr (CG=0-0.17mm)<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">C-Apochromat<br \/>\n421867-9970[1]<\/td><td class=\"column-2\">Water<\/td><td class=\"column-3\">40x<\/td><td class=\"column-4\">1.1<\/td><td class=\"column-5\">0.62*<\/td><td class=\"column-6\">DIC, Corr (CG=0.14-0.19mm)<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">C-Apochromat<br \/>\n421787-9970[1]<\/td><td class=\"column-2\">Water<\/td><td class=\"column-3\">63x<\/td><td class=\"column-4\">1.2<\/td><td class=\"column-5\">0.28*<\/td><td class=\"column-6\">DIC, Corr (CG=0.14-0.19mm)<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\">Plan-Apochromat<br \/>\n420782-9900[1]<\/td><td class=\"column-2\">Oil<\/td><td class=\"column-3\">63x<\/td><td class=\"column-4\">1.4<\/td><td class=\"column-5\">0.19<\/td><td class=\"column-6\">DIC<\/td>\n<\/tr>\n<\/tbody>\n<tfoot>\n<tr class=\"row-8\">\n\t<th class=\"column-1\">* at CG = 0.17<\/th><td class=\"column-2\"><\/td><td class=\"column-3\"><\/td><td class=\"column-4\"><\/td><td class=\"column-5\"><\/td><td class=\"column-6\"><\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Filter Cubes (turret with 6 positions)<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-32\" class=\"tablepress tablepress-id-32\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\">Filter set 49 DAPI <\/td><td class=\"column-2\">EX 365, BS 395, EM 445\/50<\/td><td class=\"column-3\">exchangeable<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\">Filter set 38 GFP<\/td><td class=\"column-2\">EX 470\/40, BS 495, EM 525\/50<\/td><td class=\"column-3\">exchangeable<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">Filter set 43 Cy3<\/td><td class=\"column-2\">EX 545\/25, BS 570, EM 605\/70<\/td><td class=\"column-3\">exchangeable<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\">BS-MP 355\/690+(R)<\/td><td class=\"column-3\">fixed position<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><\/td><td class=\"column-2\">BS-MP-760<\/td><td class=\"column-3\">fixed position<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">Rear (100% mirror)<\/td><td class=\"column-2\"><\/td><td class=\"column-3\">fixed position<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n On request (instead of one of the sets above): Filter set 50 Cy5: EX 640\/30, BS 660, EM 690\/50<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Beam splitters in scanning head<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-35\" class=\"tablepress tablepress-id-35\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Main beam splitters 1 (visible)<\/th><th class=\"column-2\">Main beam splitters 2 (invisible)<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">MBS 458<\/td><td class=\"column-2\">MBS 405<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">MBS 458\/514<\/td><td class=\"column-2\">MBS 445<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">MOBS 458\/561<\/td><td class=\"column-2\">MBS 690+<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">MBS 488<\/td><td class=\"column-2\">MBS 760+<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">MBS 488\/561<\/td><td class=\"column-2\">MBS 405\/760+<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\">MBS 488\/561\/633<\/td><td class=\"column-2\">MBS 445\/760+<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\">MBS T80\/R20<\/td><td class=\"column-2\">MBS T80\/R20<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\">Plate<\/td><td class=\"column-2\">Plate<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\">None<\/td><td class=\"column-2\">None<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Confocal scanning module LSM 880<\/h5><\/td><td class=\"column-2\">13 fps for 512x512, zoom 0.6 - 40x, max. resolution 8192 x 8192<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>FLIM Option<\/h5><\/td><td class=\"column-2\">BiG.2 and TCSPC HPM-100-40 non-descanned detectors can be on request attached to a TCSPC card (TimeHarp 260 Nano, PicoQuant)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-30 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-caab01d4-5b11-444d-80d0-d28a0ffebe67\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-caab01d4-5b11-444d-80d0-d28a0ffebe67\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-1-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Nikon CSU-W1<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-1-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"210\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2022\/11\/Nikon_CSU-W.png\" alt=\"\" class=\"wp-image-1366\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Spinning disk confocal microscope<\/li>\n\n\n\n<li>Dual camera, 7 laser wavelengths<\/li>\n\n\n\n<li>FRAP\/Photoactivation module<\/li>\n\n\n\n<li>Heating\/cooling stage top incubator<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Versatile spinning disk confocal microscope equipped with seven excitation lasers, epifluorescence LED light source, and two back-illuminated sCMOS cameras. The microscope is equipped by stage-top heating\/cooling incubator for live cell and tissue imaging and a FRAP\/photoactivation module. It is based on an inverted widefield microscope Nikon Eclipse Ti2 equipped with motorized XY stage, Perfect Focus System, semi-motorized DIC, LED transmitted light source, multicolor LED for wide-field epifluorescence, piezo stage for fast z-stack acquisition, and automatic water dispenser for long term imaging.<\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Fast multicolor confocal imaging<\/li>\n\n\n\n<li>Various measurement options: z-stack, time series, tile scan, multi positions<\/li>\n\n\n\n<li>Brightfield and DIC microscopy<\/li>\n\n\n\n<li>FRET imaging<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-a6eb6e02-5300-4314-9d2d-8f1604acf6f1\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-50afad5f-164c-4586-89c6-44ee1b11ecf6\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-50afad5f-164c-4586-89c6-44ee1b11ecf6\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-50afad5f-164c-4586-89c6-44ee1b11ecf6\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-50afad5f-164c-4586-89c6-44ee1b11ecf6\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-19\" class=\"tablepress tablepress-id-19\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">NIS-Elements<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Spinning disk module<\/h5><\/td><td class=\"column-2\">Yokogawa CSU-W1<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Pinhole size [\u03bcm] <br \/>\n    (dual disk)<\/h5><\/td><td class=\"column-2\">25, 50<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Laser excitation wavelengths [nm] <br \/>\n(all CW)<\/h5><\/td><td class=\"column-2\">405, 445, 488, 515, 561, 594, 640 <\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Dichroic mirrors (spinning disk module)<\/h5><\/td><td class=\"column-2\">quad-band dichroic mirror 405\/488\/561\/638 nm <br \/>\ntripple-band dichroic mirror 445\/514\/594 nm <br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Image splitting mirrors (spinning disk module)<\/h5><\/td><td class=\"column-2\">561 nm long-pass dichroic mirror (DM A561LP)<br \/>\n514 nm long-pass dichroic mirror (DM A514LP)<br \/>\nempty position for single camera acquisition<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Filter cubes (microscope)<\/h5><\/td><td class=\"column-2\">DAPI\/FITC\/TRITC\/Cy5<br \/>\nCFP\/YFP\/mCherry<br \/>\nFITC<br \/>\nTRITC<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Emission filters<\/h5><\/td><td class=\"column-2\">455\/50, 480\/40m, 525\/50, 545\/40, 600\/50, 645\/75, 690\/50, 720\/60, 455\/50, 480\/40, 525\/50, 545\/40, 440\/521\/607\/700 nm quad-band bandpass filter, <br \/>\n475\/543\/702 nm triple-band bandpass filter, <br \/>\nDual Line Emitter 405 \/ 488<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-28\" class=\"tablepress tablepress-id-28\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Objective Name<\/th><th class=\"column-2\">Magnitude<\/th><th class=\"column-3\">Immersion<\/th><th class=\"column-4\">N.A.<\/th><th class=\"column-5\">WD[MM]<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">CFI Plan Apo Lambda 10X<\/td><td class=\"column-2\">10<\/td><td class=\"column-3\">air<\/td><td class=\"column-4\">0.45<\/td><td class=\"column-5\">4<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">CFI Plan Apo VC 20x<\/td><td class=\"column-2\">20<\/td><td class=\"column-3\">air<\/td><td class=\"column-4\">0.75<\/td><td class=\"column-5\">1<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">CFI Plan Apo Lambda S 25XC Sil<\/td><td class=\"column-2\">25<\/td><td class=\"column-3\">silicone oil<\/td><td class=\"column-4\">1.05<\/td><td class=\"column-5\">0.55<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">CF Plan Apo VC 60XC WI<\/td><td class=\"column-2\">60<\/td><td class=\"column-3\">water<\/td><td class=\"column-4\">1.20<\/td><td class=\"column-5\">0.31-0.28<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">CFI SR HP Plan Apo Lambda S 100XC Sil<\/td><td class=\"column-2\">100<\/td><td class=\"column-3\">silicone oil<\/td><td class=\"column-4\">1.35<\/td><td class=\"column-5\">0.31-0.28<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\">CFI Plan Apo VC 100X Oil<\/td><td class=\"column-2\">100<\/td><td class=\"column-3\">oil<\/td><td class=\"column-4\">1.40<\/td><td class=\"column-5\">0.13<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><h5>Epifluorescence source<\/h5><\/td><td class=\"column-2\">CoolLED pE-4000 (365, 385, 405, 435, 460, 470, 490, 500, 525, 550, 580, 595, 635, 660, 740, and 770 nm)<\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\"><h5>Tube lenses<\/h5><\/td><td class=\"column-2\">1x, 1.5x<\/td>\n<\/tr>\n<tr class=\"row-12\">\n\t<td class=\"column-1\"><h5>Relay lens magnification<\/h5><\/td><td class=\"column-2\">1x, 2x, motorized changer<\/td>\n<\/tr>\n<tr class=\"row-13\">\n\t<td class=\"column-1\"><h5>Camera (2x)<\/h5><\/td><td class=\"column-2\">PRIME BSI (Teledyne Photometrics)<br \/>\n  <p style=\"margin-left: 20px; margin-bottom: 0px \";>  2048 x 2048 pixels<br \/>\n     pixel size: 6.5 x 6.5 \u03bcm<br \/>\n     63 fps @ 2048 x 2048 pixels, 11-bit data<br \/>\n     QEmax> 95% <\/p><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-19 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-50afad5f-164c-4586-89c6-44ee1b11ecf6\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-50afad5f-164c-4586-89c6-44ee1b11ecf6\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-2-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Abberior Instruments STED &#8211; INFINITY<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-2-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" data-id=\"2724\" src=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/12\/abberior_sted_infinity.jpg\" alt=\"\" class=\"wp-image-2724\" srcset=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/12\/abberior_sted_infinity.jpg 1024w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/12\/abberior_sted_infinity-300x225.jpg 300w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/12\/abberior_sted_infinity-768x576.jpg 768w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/12\/abberior_sted_infinity-400x300.jpg 400w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>\n<\/li><\/ul>\n<\/div>\n<\/div>\n\n\n\n<ul class=\"wp-block-list\">\n<li>3 dedicated 60x STED objectives (water, oil, and silicone)<\/li>\n\n\n\n<li>5 excitation lines (405, 440, 485, 561, and 640 nm)<\/li>\n\n\n\n<li>Pulsed ultra-high-power STED laser (775 nm)<\/li>\n\n\n\n<li>3 APD-based spectral detection channels<\/li>\n\n\n\n<li>2 Spectral MATRIX detector (APD array) allowing differential detection on two channels<\/li>\n\n\n\n<li>TIMEBOW for measuring fluorescence lifetime with and without super resolution<\/li>\n\n\n\n<li>Adaptive optics for STED beams based on SLM technology<\/li>\n\n\n\n<li>Adaptive optics system (DFM deformable mirror) for all beams (excitation, emission, and depletion)<\/li>\n\n\n\n<li>Full auto-alignment<\/li>\n\n\n\n<li>Continuous hardware autofocus for STED &amp; confocal<\/li>\n\n\n\n<li>Adaptive illumination package for highest resolution and live-cell super-resolution imaging at ultra-low light levels (RESCue,<\/li>\n\n\n\n<li>DyMIN, MINFIELD)<\/li>\n<\/ul>\n\n\n\n<p>For more detailed microscope characteristics please see the section \u201cMicroscope\u201d. Basic introduction to STED microscopy can be found here:<\/p>\n\n\n\n<p><a href=\"https:\/\/abberior-instruments.com\/knowledge\/microscopy-tutorials\/sted\/\">https:\/\/abberior-instruments.com\/knowledge\/microscopy-tutorials\/sted\/<\/a><\/p>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<table id=\"tablepress-75\" class=\"tablepress tablepress-id-75\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\"><ul><li>Lightbox Software for Facility Line<\/li><li>ImSpector<\/li><li>TIMEBOW Software Package for Lightbox<\/li><ul><\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Epifluorescence<\/h5><\/td><td class=\"column-2\">CoolLED pE-4000, 16 LEDs<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Epifluorescence cubes<\/h5><\/td><td class=\"column-2\"><ul><li>FITC<br \/>\n<li>TRITC<br \/>\n<li>QUAD (DAPI\/FITC\/Cy3\/Cy5)<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Excitation lasers<\/h5><\/td><td class=\"column-2\"><ul><li>405 nm<br \/>\n<li>440 nm<br \/>\n<li>485 nm<br \/>\n<li>561 nm<br \/>\n<li>640 nm<br \/>\n<br \/>\n<li>All lasers are pulsed<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>STED Laser<\/h5><\/td><td class=\"column-2\">775 nm (40 MHz pulsed laser, for 2D and 3D STED)<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Donut formation<\/h5><\/td><td class=\"column-2\">Programmable Spatial Light Modulator \u2013 775 nm<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Scanner<\/h5><\/td><td class=\"column-2\">Abberior QUAD scanner<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\"><ul><li>Olympus\/Evident UPLXAPO10X, 10x\/NA 0.4 Dry, WD 3.1mm<br \/>\n<li>Olympus\/Evident UPLXAPO20X, 20x\/NA 0.8 Dry, WD 0.6mm<br \/>\n<li>Olympus\/Evident UPLSAPO30XS, 30x\/NA 1.05, silicone oil, WD 0.8mm<br \/>\n<li>Olympus\/Evident UPLSAPO60XW, 60x\/NA 1.2, Water, WD 0.28mm<br \/>\n<li>Olympus\/Evident UPLSAPO60XS, 60x\/NA 1.3, Silicone Oil, WD 0.3mm<br \/>\n<li>Olympus\/Evident UPLXAPO60XO, 60x\/NA 1.42, Oil, WD 0.15mm<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><h5>Rainbow Spectral Detection Kit<\/h5><\/td><td class=\"column-2\"><ul><li>Low-loss, background-free, variable spectral detection<br \/>\n<li>Detection bands fully tunable between 400 and 800nm<br \/>\n<li>No dispersive elements required<br \/>\n<li>Fully integrated in Lightbox software, detection bands can be selected at an instant<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><h5>Pinholes<\/h5><\/td><td class=\"column-2\">16 pinholes from 25 \u03bcm to 2 mm<\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\"><h5>Detectors<\/h5><\/td><td class=\"column-2\"><ul><li>3x Single-photon-counting APD with a superior sensitivity (up to 65% photon detection efficiency)<br \/>\n<li>2x MATRIX detector (consist of multiple avalanche photo diodes with quantum efficiency over 50 % at 500 nm in a hexagonal arrangement on a single detector chip, allows pixel re-assignment and differential detection)<\/td>\n<\/tr>\n<tr class=\"row-12\">\n\t<td class=\"column-1\"><h5>FLIM<\/h5><\/td><td class=\"column-2\"><ul><li>Time-correlated single photon counting (TCSPC) hardware: High-throughput multichannel event timer &amp; TCSPC unit (MultiHarp 150)<br \/>\n<li>Up to 5 FLIM detection channels (3 APD channels + 2 MATRIX detection channels)<br \/>\n<li>Direct photon streaming to .ptu format available for APD channels<br \/>\n<li>Phasor plot analysis available in Lightbox<\/td>\n<\/tr>\n<tr class=\"row-13\">\n\t<td class=\"column-1\"><h5>RAYSHAPE Adaptive Optics system<\/h5><\/td><td class=\"column-2\"><ul><li>Deformable mirror-based correction of arbitrary aberrations for all<br \/>\nbeams<br \/>\n<li>More than 140 elements with a stroke of several microns ensure precise correction, even of high-order aberrations<br \/>\n<li>Compensates for sample-induced aberrations, e.g. when focusing into a refractive-index mismatched sample, or into samples with internal inhomogeneities<br \/>\n<li>Applied spherical correction changes with focus to ensure automatic correction at all focal depths<br \/>\n<li>No moving elements in the objective<br \/>\n<li>Enables superior signal and resolution, in particular with 3DSTED, but also for confocal and multiphoton images, e.g. of thick tissue samples<\/td>\n<\/tr>\n<tr class=\"row-14\">\n\t<td class=\"column-1\"><h5>FLEXPOSURE Adaptive Illumination<\/h5><\/td><td class=\"column-2\"><ul><li>Light dose minimization by up to two orders of magnitude to leverage live cell imaging<br \/>\n<li>Bleaching reduction applicable to all fluorescent markers<br \/>\n<li>Based on concurrent, pixel-wise decision-making of whether structure is present in the current pixel<br \/>\n<li>RESCue, DyMIN, MINFIELD, see <a href=\"https:\/\/abberior.rocks\/superresolution-confocal-systems\/modules\/flexposure-illumination\/\">https:\/\/abberior.rocks\/superresolution-confocal-systems\/modules\/flexposure-illumination\/<\/a><br \/>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-75 from cache -->\n\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-3-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Abberior Instruments STED<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-3-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"210\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/abberior-regular280.jpg\" alt=\"\" class=\"wp-image-357\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Inverted confocal microscope<\/li>\n\n\n\n<li>STED super-resolution microscopy<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Fluorescence inverted confocal microscope and STED super-resolution nanoscope with high detection efficiency of far-red photons. The system is equipped with four excitation lasers, depletion laser 775\u00a0nm and four single photon counting detectors. For more detailed microscope characteristics please see the section \u201cMicroscope\u201d. Basic introduction to STED microscopy can be found here:<\/p>\n\n\n\n<p><a href=\"https:\/\/abberior-instruments.com\/knowledge\/microscopy-tutorials\/sted\/\">https:\/\/abberior-instruments.com\/knowledge\/microscopy-tutorials\/sted\/<\/a><\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Two-color 2D and 3D super-resolution images obtained by STED technique with a\u00a0pulsed depletion laser 775 nm and pulsed excitation 561 nm and 640 nm\u00a0<\/li>\n\n\n\n<li>STED RESCUE and DYMIN mode available (STED imaging mode that significantly reduces the light dose sent onto the sample without compromising the resolution)<\/li>\n\n\n\n<li>2D and 3D STED images available also with water immersion objective<\/li>\n\n\n\n<li>Multicolor confocal\u00a0scanning system with variable pinhole size<\/li>\n\n\n\n<li>Possible FLIM or FCS acquisition<\/li>\n\n\n\n<li>Live cell imaging<\/li>\n\n\n\n<li>NIR confocal imaging (775\u00a0nm excitation)<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>Inverted confocal microscope Nikon Eclipse Ti-E equipped with a piezo Z-stage, motorized XY stage, Perfect Focus System, transmitted light lamp (100 W) and following units:<\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-6a8b4edc-8827-4219-b12e-4bce3af2d728\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-f81f5397-a8d1-489a-bc54-56da4febc12a\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-f81f5397-a8d1-489a-bc54-56da4febc12a\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-f81f5397-a8d1-489a-bc54-56da4febc12a\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-f81f5397-a8d1-489a-bc54-56da4febc12a\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-24\" class=\"tablepress tablepress-id-24\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">Imspector (v16.3)<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Epifluorescence<\/h5><\/td><td class=\"column-2\">CoolLED pE-4000<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Epifluorescence cubes<\/h5><\/td><td class=\"column-2\">FITC (465-495, DM505, 515-555)<br \/>\nTRITC (527-553, DM565, 577-633)<br \/>\nQUAD (DAPI\/FITC\/Cy3\/Cy5)<br \/>\nAnalyzer<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Excitation lasers<\/h5><\/td><td class=\"column-2\">cw - 405 nm, pulsed - 485 nm, 561 nm, 640 nm, and 775 nm<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>STED Laser<\/h5><\/td><td class=\"column-2\">775 nm (40 MHz pulsed laser, for 2D and 3D STED)<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Donut formation<\/h5><\/td><td class=\"column-2\">Programmable Spatial Light Modulator \u2013 775 nm<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Scanner<\/h5><\/td><td class=\"column-2\">Abberior QUAD scanner: line frequency up to 2.5 kHz<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\">Nikon CFI Plan Apo Lambda 60x Oil, NA 1.40, WD 0.13 mm<br \/>\nNikon CFI Plan Apo 60x WI, NA 1.27, WD 0.17 mm, Correction Collar 0.15-0.19, DIC <br \/>\nNikon CFI Plan Apo Lambda 10x, NA 0.45, WD 4 mm, DIC <\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><h5>Emission filters<\/h5><\/td><td class=\"column-2\">422-467 nm<br \/>\n506-594 nm (or 500-550 nm)<br \/>\n580-630 nm<br \/>\n650-720 nm (or 800-870 nm)<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><h5>Pinholes<\/h5><\/td><td class=\"column-2\">16 pinholes from 25 \u03bcm to 2 mm<\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\"><h5>Detectors<\/h5><\/td><td class=\"column-2\">4x Photon Counting Module (Excelitas Technologies)<br \/>\n<ul style=\"list-style-type:circle\"><li>Two time resolved detectors optimized for FLIM<\/li><li>High detection sensitivity for red light (>62% for 680nm)<\/li><\/ul>PMT for transmitted and reflected light (Hamamatsu Photonics)<br \/>\nMonochrome widefield camera DMK 23U274 (Imaging Source)<ul><li>CCD sensor, 1600\u00d71200 (1.9 MP), up to 20 fps, pixel size 4.4 \u00b5m x 4.4 \u00b5m<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-12\">\n\t<td class=\"column-1\"><h5>FLIM<\/h5><\/td><td class=\"column-2\">Externally connected TCSPC card: TimeHarp260N (PicoQuant)<ul><li>Two independent channels, ultra-short dead time <2ns, temporal resolution 250 ps<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-24 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-f81f5397-a8d1-489a-bc54-56da4febc12a\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-f81f5397-a8d1-489a-bc54-56da4febc12a\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-4-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Carl Zeiss Lattice Lightsheet 7<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-4-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/12\/LLS.png\" alt=\"\" class=\"wp-image-2750\" srcset=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/12\/LLS.png 1024w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/12\/LLS-300x225.png 300w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/12\/LLS-768x576.png 768w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/12\/LLS-400x300.png 400w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Short term, long term live cell imaging at subcellular resolution<\/li>\n\n\n\n<li>Low phototoxicity and photobleching<\/li>\n\n\n\n<li>Dual camera setup for colocalization studies<\/li>\n\n\n\n<li>High speed volumetric imaging, penetration depth up to 200 \u00b5m<\/li>\n\n\n\n<li>Tissue culture cells, cells in hydrogel up to spheroids and organoids<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Lightsheet microscope with gentle light sheet imaging at high resolution and volumetric speed with standard sample carriers. Complex alignment processes are performed automatically on workstation with high-speed data transport connection.<\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<p>Volumetric live cell imaging of:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><ul><li>adherent or suspension cells<\/li><\/ul><ul><li>3D cell cultures: cysts, cells in hydrogel, organoids, spheroids with diameter up to 200\u00b5m<\/li><\/ul><ul><li>Small evolving organisms: zebrafish embryo, C.elegans embryo, Drosophila embryo<\/li><\/ul><\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-54befce4-a047-419a-ae97-83a5686c0de6\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-38fe7807-8820-40e5-9d7b-0657d51cc22a\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-38fe7807-8820-40e5-9d7b-0657d51cc22a\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-38fe7807-8820-40e5-9d7b-0657d51cc22a\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-38fe7807-8820-40e5-9d7b-0657d51cc22a\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-76\" class=\"tablepress tablepress-id-76\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">ZEN 3.6 (blue edition)<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Illumination<\/h5><\/td><td class=\"column-2\"><ul><li>LED (white &amp; red) for transmitted lightE<br \/>\n<li>Laser (488 nm, 561nm, 640 nm) for beam adjustment and fluorescence imaging<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Cameras<\/h5><\/td><td class=\"column-2\">2x Hamamatsu Orca-Fusion sCMOS camera<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\">Illumination: 13.3x\/ NA 0.4<br \/>\nDetection: 44.83x \/ NA 1.0<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Filters<\/h5><\/td><td class=\"column-2\">Emission Filter Camera 1<br \/>\n<ul><li>BP 570-620 + LP 655<br \/>\n<li>BP 495-550 + LP 655<br \/>\n<li>LBF 405\/488\/561\/642<br \/>\n<li>ND filter<br \/>\n<li>Empty<br \/>\n<li>BP 495-570<br \/>\n<li>LP 570<\/li><\/ul><br \/>\nEmission Filter Camera 2<br \/>\n<ul><li>BP 570-610 IR+<br \/>\n<li>Empty<br \/>\n<li>BP 495-550 + BP 570-620<br \/>\n<li>BP 500-550 IR+<\/li><\/ul><br \/>\nSecondary Beam Splitter<br \/>\n<ul><li>Plate<br \/>\n<li>LP 565<br \/>\n<li>LP 640<br \/>\n<li>Empty<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Resolution<\/h5><\/td><td class=\"column-2\">Deskewed: 330 nm \u00d7 330 nm \u00d7 500 \u2013 1000 nm;<br \/>\nDeskewed w\/DCV: 290 nm \u00d7 290 nm \u00d7 450 nm\u2013 900 nm (z-res. = light sheet thickness if \u22641000 nm)<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Light sheets<\/h5><\/td><td class=\"column-2\">Beam shaping by cylinder lens and spatial light modulator (SLM)<br \/>\nPre-defined Sinc3 beams with length [\u03bcm] \u00d7 thickness [nm]:<br \/>\n<ul><li>15 \u00d7 550 (w\/side lobes) &amp; 15 \u00d7 650 (w\/o side lobes)<br \/>\n<li>30 \u00d7 700 (w\/side lobes) &amp; 30 \u00d7 1,000 (w\/o side lobes)<br \/>\n<li>100 \u00d7 1,400 (w\/side lobes) &amp; 100 \u00d7 1,800 (w\/o side lobes)<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Acquisition rate<\/h5><\/td><td class=\"column-2\">Volume: 3 Vol\/s @ approx. 300 \u03bcm \u00d7 50 \u03bcm \u00d7 20 \u03bcm<br \/>\nPlane: 400 frames\/s @ approx. 300 \u03bcm \u00d7 20 \u03bcm<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><h5>Sample carrier frames<\/h5><\/td><td class=\"column-2\"><ul><li>\"Dish 35\": for 35 mm cell culture dishes<br \/>\n<li>\"Dish 35...40\": for 35 \u2013 40 mm cell culture dishes<br \/>\n<li>\"Slide\": for slides 26 mm \u00d7 76 mm; also suitable for multi-well glass-bottom slides 26 mm \u00d7 76 mm<br \/>\n<li>\"Chamber slide\": for LabTekR chambers 25 mm \u00d7 57 mm;<br \/>\nalso suitable for multi-well glass-bottom slides 25 mm \u00d7 57 mm<br \/>\n<li>\"Multiwell\": for multiwell microplates 85.48 mm \u00d7 127.76 mm<\/ul><\/li><\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><h5>Environmental control<\/h5><\/td><td class=\"column-2\">Heating system, gas mixer system (CO2 and N2 level control) - integrated in the software<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-76 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-38fe7807-8820-40e5-9d7b-0657d51cc22a\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-38fe7807-8820-40e5-9d7b-0657d51cc22a\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-5-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong><strong>Nikon iLas 2 ring-TIRF\/FRAP<\/strong><\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-5-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"360\" height=\"270\" src=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/07\/ringTIRF.jpg\" alt=\"\" class=\"wp-image-2578\" srcset=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/07\/ringTIRF.jpg 360w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/07\/ringTIRF-300x225.jpg 300w\" sizes=\"auto, (max-width: 360px) 100vw, 360px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Inverted widefield microscope<\/li>\n\n\n\n<li>ring-TIRF illumination<\/li>\n\n\n\n<li>Image splitter (optional)<\/li>\n\n\n\n<li>Two cameras (sCMOS, EM-CCD)<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Inverted fluorescence widefield microscope based on Nikon ECLIPSE Ti2 microscope body and iLas 2 (Gataca Systems) illumination device allowing ring-TIRF microscopy and FRAP.<\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Fast  multicolor widefield imaging with TIRF or HILO excitation option<\/li>\n\n\n\n<li>Bright-field microscopy<\/li>\n\n\n\n<li>Epi-fluorescence<\/li>\n\n\n\n<li>Interference reflection microscopy<\/li>\n\n\n\n<li>FRAP (fluorescence recovery after photobleaching) measurements<\/li>\n\n\n\n<li>Option of simultaneous dual wavelength imaging by single camera\u00a0<\/li>\n\n\n\n<li>Long term live-cell imaging available<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>Inverted widefield microscope body Nikon Eclipse Ti2, motorized XY stage, Perfect Focus System, automatized H-TIRF module, insertable gradation neutral density filter, transmitted light lamp (100 W) and following units:<\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-9d999eab-3612-41e9-a1f2-e47a39ed3b3f\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-7dfcbb43-28fe-4519-b129-2d7e0dee0a6b\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-7dfcbb43-28fe-4519-b129-2d7e0dee0a6b\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-7dfcbb43-28fe-4519-b129-2d7e0dee0a6b\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-7dfcbb43-28fe-4519-b129-2d7e0dee0a6b\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-73\" class=\"tablepress tablepress-id-73\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">NIS-Elements<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Epifluorecence<\/h5><\/td><td class=\"column-2\">CoolLED pE-300, 400 nm, 460 nm, 550 nm<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Laser excitation wavelengths<\/h5><\/td><td class=\"column-2\">405 nm, 488 nm, 561 nm, 640 nm (all cw)<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\"><ul><li>Nikon Plan Apo \u03bb 20x, NA 0.75, WD 1.00 mm<\/li><li>Nikon Apo TIRF 60x Oil, NA 1.49, WD 0.12 mm, Correction Collar 0.15-0.19, DIC<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Filter cubes<\/h5><\/td><td class=\"column-2\"><ul><li>Chroma TRF89901v2 ET - 405\/488\/561\/640nm Laser Quad Band Set for TIRF applications<\/li><li>TIRF LF488\/LP-C Filtrblok<\/li><li>TIRF LF561\/LP-C Filtrblok<\/li><li> Epi-Fl C-FL-HQ Filtrblok <br \/>\nF21-020 50R\/50T<\/li><li>Beamsplitter 400-1000<\/li><li>LED-DA\/FI\/TR\/Cy5-B Filter Cube<\/li><br \/>\n<\/ul><\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Filter turret<\/h5><\/td><td class=\"column-2\"><ul><li>465\/30 nm BrightLine single-band bandpas<\/li><li>525\/45 nm BrightLine single-band bandpas<\/li><li>600\/37 nm BrightLine single-band bandpas<\/li><li>697\/58 nm BrightLine single-band bandpas<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Tube lenses<\/h5><\/td><td class=\"column-2\">1x, 1.5x<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Camera<\/h5><\/td><td class=\"column-2\">PRIME BSI (Teledyne Photometrics)<br \/>\n<ul><li>2048 x 2048 pixels<\/li><li>pixel size: 6.5 x 6.5 \u03bcm<\/li><li>63 fps @ 2048 x 2048 pixels, 11-bit data<\/li><li>QEmax> 95%<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-73 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-7dfcbb43-28fe-4519-b129-2d7e0dee0a6b\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-7dfcbb43-28fe-4519-b129-2d7e0dee0a6b\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-6-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Nikon N-STORM<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-6-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"210\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/nikon_simnstorm-regular280.jpg\" alt=\"\" class=\"wp-image-363\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Inverted widefield microscope<\/li>\n\n\n\n<li>TIRF illumination<\/li>\n\n\n\n<li>Image splitter<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Multifunctional fluorescence inverted widefield microscope enabling live-cell imaging, TIRF or HILO illumination, and single molecule localization microscopy (SMLM) superresolution technique.<\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Single Molecule Localization Microscopy methods (dSTORM, PALM, DNA-PAINT)<\/li>\n\n\n\n<li>Two-color dSTORM images acquired by spectral demixing<\/li>\n\n\n\n<li>Fast and sensitive multicolor widefield imaging with TIRF or HILO excitation option<\/li>\n\n\n\n<li>Option of simultaneous dual wavelength imaging by single camera\u00a0<\/li>\n\n\n\n<li>Long term live-cell imaging available<\/li>\n\n\n\n<li>Brightfield microscopy<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>Inverted widefield microscope Nikon Eclipse Ti-E equipped with a piezo Z-stage, motorized XY stage, Perfect Focus System, automatized H-TIRF module, insertable quarter-wave plate, insertable gradation neutral density filter, transmitted light lamp (100 W) and following units:<\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-f7c1118a-aece-4741-b1ec-3cda65b615cb\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-6d4b3e23-d01b-4d41-ba13-3895c8d1b564\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-6d4b3e23-d01b-4d41-ba13-3895c8d1b564\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-6d4b3e23-d01b-4d41-ba13-3895c8d1b564\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-6d4b3e23-d01b-4d41-ba13-3895c8d1b564\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-20\" class=\"tablepress tablepress-id-20\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">NIS-Elements (v5.21)<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Epifluorecence<\/h5><\/td><td class=\"column-2\">Nikon Intensilight E<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Laser excitation wavelengths<\/h5><\/td><td class=\"column-2\">405 nm, 445nm, 488 nm, 561 nm, 647 nm (all cw)<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\"><ul><li>Nikon CFI Plan Apochromat Lambda D 4X, NA 0.2, WD 20 mm<\/li><li>Nikon CFI HP Apo TIRF 100x Oil, NA 1.49, WD 0.12 mm, Temperature Correction Ring 23-37\u00b0C (for STORM)<\/li><li style= \"margin-top : 0px; margin-bottom: 0px\">Nikon CFI SR Apo TIRF 100x Oil, NA 1.49, WD 0.12 mm, Temperature Correction Ring 23-37\u00b0C<\/li><li>Nikon CFI Plan Apo 60x WI, NA 1.27, WD 0.17 mm, Correction Collar 0.15-0.19 (for SIM)<\/li><li>Nikon CFI Plan Apo Lambda 20x, NA 0.75, WD 1mm<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Cameras<\/h5><\/td><td class=\"column-2\">EM CCD Andor iXon Ultra DU897 (Andor Technologies)<ul><li>right port<\/li><li>512x512 pixels<\/li><li>pixel size: 16x16 \u03bcm<\/li><li>frame rate 56 fps for 512x512 pixels, standard mode<\/li><\/ul><br \/>\nsCMOS Hamamatsu ORCA 4.0 V2 (Hamamatsu Photonics)<ul><li>left port<\/li><li>2048x2048 pixels<\/li><li>pixel size: 6.5x6.5 \u03bcm<\/li><li>frame rate 100 fps for 2048x2048 pixels, standard scan<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Filter turret<\/h5><\/td><td class=\"column-2\">DAPI (361-389, DM415, 430-490)<br \/>\nCFP TIRF (436\/20, 442rdc, 460lp, 480\/40) \u2013 inserted on demand<br \/>\n488 TIRF (488\/10, 488rdc, 500lp, 525\/50)<br \/>\n561 TIRF (561\/10, 561rdc, 575lp, 600\/50)<br \/>\nCy5 (628\/40, DM660, 731\/137) \u2013 inserted on demand<br \/>\ncontinuous STORM TIRF<br \/>\n(excitation, mirror, emission:<br \/>\n387-417, 420-481, 422-478<br \/>\n483-494, 497-553, 502-549<br \/>\n557-570, 575-628, 581-625<br \/>\n636-661, 667-792, 674-786)<br \/>\nHM Half Mirror<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Tube lenses<\/h5><\/td><td class=\"column-2\">1x, 1.5x<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Relay lenses in front of ORCA Camera<\/h5><\/td><td class=\"column-2\">1x (widefield), 0.4x (STORM)<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><h5>Relay lenses in front of EM CCD Camera<\/h5><\/td><td class=\"column-2\">1x (astigmatism lens for STORM)<br \/>\n1x (widefield)<br \/>\n2.5x (SIM)<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><h5>Astigmatism lens (for 3D STORM)<\/h5><\/td><td class=\"column-2\">Yes, insertion possible for both cameras<\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\"><h5>Image splitter<\/h5><\/td><td class=\"column-2\">DV2 (Photometrics)<ul><li>inserted on demand<\/li><li>available beamsplitters: 565 nm (T565lpxr), 505 nm (T505lpxr), Polarization\/Anisotropy module<\/li><\/ul> <br \/>\nW-VIEW GEMINI (Hamamatsu Photonics)<ul><li>inserted on demand<\/li><li>available beamsplitters for 484 nm, 560 nm, 640 nm, and 700 nm<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-20 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-6d4b3e23-d01b-4d41-ba13-3895c8d1b564\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-6d4b3e23-d01b-4d41-ba13-3895c8d1b564\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-7-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Nikon Ti-E H-TIRF<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-7-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"210\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/nikon_h-tirf-regular280.jpg\" alt=\"\" class=\"wp-image-364\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Inverted widefield microscope<\/li>\n\n\n\n<li>TIRF illumination<\/li>\n\n\n\n<li>Image splitter<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Versatile fluorescence inverted widefield microscope equipped with four lasers, epifluorescence lamp and EM-CCD and CMOS cameras. The microscope is designed for short and long live-cell imaging and experiments under standard or TIRF illumination.\u00a0Link to manufacturer website:<\/p>\n\n\n\n<p><a href=\"https:\/\/www.nikoninstruments.com\/cz_CZ\/Vyrobky\/Inverzni-mikroskopy\/Eclipse-Ti-E\">https:\/\/www.nikoninstruments.com\/cz_CZ\/Vyrobky\/Inverzni-mikroskopy\/Eclipse-Ti-E<\/a><a href=\"\"><\/a><\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Fast and sensitive multicolor widefield imaging with TIRF, HILO or EPI excitation option<\/li>\n\n\n\n<li>Option of simultaneous dual wavelength imaging by single camera\u00a0<\/li>\n\n\n\n<li>Short or long term live-cell imaging available<\/li>\n\n\n\n<li>Various measurement options: z-stack, time series, tile scan, multi positions<\/li>\n\n\n\n<li>Brightfield and DIC microscopy<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>Inverted widefield microscope Nikon Eclipse Ti-E equipped with motorized XY stage, Perfect Focus System, H-TIRF module, module for environmental control Okolab (temperature controlled range room temperature up to 40\u00b0C; CO2 0-10%; humidity up to 95 %), insertable quarter-wave plate, insertable gradation neutral density filter, transmitted light lamp (100 W) and following units:<\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-fd4f1aeb-10e5-4804-ac7c-bce35317b7ea\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-fd4f1aeb-10e5-4804-ac7c-bce35317b7ea\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-fd4f1aeb-10e5-4804-ac7c-bce35317b7ea\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-fd4f1aeb-10e5-4804-ac7c-bce35317b7ea\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-fd4f1aeb-10e5-4804-ac7c-bce35317b7ea\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-21\" class=\"tablepress tablepress-id-21\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">NIS-Elements Ar (v5.02)<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Epifluorecence<\/h5><\/td><td class=\"column-2\">Nikon Intensilight E<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Laser excitation wavelengths<\/h5><\/td><td class=\"column-2\">405 nm, 488 nm, 561 nm, 640 nm (all cw)<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\"><ul><li>Nikon CFI Apo TIRF 60x Oil, NA 1.49, WD 0.12 mm, Correction Collar, DIC (motorized prism insertion)<\/li><li>Nikon CFI Plan Apo 60x WI, NA 1.27, WD 0.17 mm, Correction Collar 0.15-0.19, DIC<\/li> <li>Nikon CFI Plan Apo Lambda 20x, NA 0.75, WD 1mm, DIC<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Cameras<\/h5><\/td><td class=\"column-2\">EM CCD Andor iXon Ultra DU888 (Andor Technologies)<ul><li>right port<\/li><li>1024 x 1024 pixels<\/li><li>pixel size: 13 \u03bcm x 13 \u03bcm<\/li><li>26 fps at 1024 x 1024 pixels, QEmax> 90%<\/li><\/ul><br \/>\n<br \/>\nPRIME BSI (Teledyne Photometrics)<br \/>\n<ul><li>2048 x 2048 pixels<\/li><li>pixel size: 6.5 x 6.5 \u03bcm<\/li><li>63 fps @ 2048 x 2048 pixels, 11-bit data<\/li><li>QEmax> 95%<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Filter turret<\/h5><\/td><td class=\"column-2\">TagBFP (excitation 395-415, emission 435-485)<br \/>\nEGFP (483-493, 500-550)<br \/>\nmCherry (556-566, 593-668)<br \/>\nCy5 (632-652, 669-741) <br \/>\nQuad Band Set - 405\/488\/561\/640<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Tube lenses<\/h5><\/td><td class=\"column-2\">1x, 1.5x<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Image splitter<\/h5><\/td><td class=\"column-2\">W-View Gemini (Hamamatsu Photonics)<ul><li>in front of Hamamatsu camera<\/li><li>available beamsplitters: 484 nm, 560 nm, 640 nm and 700 nm<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-21 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-fd4f1aeb-10e5-4804-ac7c-bce35317b7ea\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-fd4f1aeb-10e5-4804-ac7c-bce35317b7ea\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-8-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Nikon Ti-E H-TIRF 2<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-8-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"210\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/img_20200128_094704-regular280.jpg\" alt=\"\" class=\"wp-image-365\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Inverted widefield microscope<\/li>\n\n\n\n<li>TIRF illumination<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Versatile fluorescence inverted widefield microscope equipped with three lasers, epifluorescence lamp, and back-illuminated sCMOS camera. The microscope is designed for short- and long-term imaging experiments under standard or TIRF illumination.\u00a0Links to manufacturer website:<\/p>\n\n\n\n<p><a href=\"https:\/\/www.microscope.healthcare.nikon.com\/products\/inverted-microscopes\/eclipse-ti2-series\">https:\/\/www.microscope.healthcare.nikon.com\/products\/inverted-microscopes\/eclipse-ti2-series<\/a><a href=\"\"><\/a><\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Fast and sensitive multicolor widefield imaging with TIRF, HILO or EPI excitation option<\/li>\n\n\n\n<li>Various measurement options: z-stack, time series, tile scan, multi positions<\/li>\n\n\n\n<li>Brightfield and DIC microscopy<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>Inverted widefield microscope Nikon Eclipse Ti2 equipped with motorized XY stage, Perfect Focus System, H-TIRF module, insertable quarter-wave plate, insertable gradation neutral density filter, transmitted light lamp (Nikon TI2-D-LHLED) and following units:<\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-f250e580-0f18-4ab0-bde4-1c00ba96d0ed\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-f250e580-0f18-4ab0-bde4-1c00ba96d0ed\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-f250e580-0f18-4ab0-bde4-1c00ba96d0ed\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-f250e580-0f18-4ab0-bde4-1c00ba96d0ed\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-f250e580-0f18-4ab0-bde4-1c00ba96d0ed\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-22\" class=\"tablepress tablepress-id-22\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">NIS-Elements AR (v. 5.20)<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Epifluorecence<\/h5><\/td><td class=\"column-2\">CoolLED pE-300, 400 nm, 460 nm, 550 nm<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Laser excitation wavelengths<\/h5><\/td><td class=\"column-2\">488 nm, 561 nm, 640 nm (all cw)<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Filter turret<\/h5><\/td><td class=\"column-2\">EM435-485<br \/>\nEM500-545<br \/>\nEM610\/75<br \/>\nEM700\/75<br \/>\nQuad Band Set - 405\/488\/561\/640<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\"><ul><li>Nikon CFI Plan Apo Lambda 10x, NA 0.45, WD 4 mm<\/li><li>Nikon Plan Apo \u03bb 20x, NA 0.75, WD 1.00 mm<\/li><li>Nikon Apo TIRF 60x Oil, NA 1.49, WD 0.12 mm, Correction Collar 0.15-0.19, DIC<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Tube lenses<\/h5><\/td><td class=\"column-2\">1x, 1.5x<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Relay lens (mountable)<\/h5><\/td><td class=\"column-2\">2.5x<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Camera<\/h5><\/td><td class=\"column-2\">PRIME BSI (Teledyne Photometrics)<br \/>\n<ul><li>2048 x 2048 pixels<\/li><li>pixel size: 6.5 x 6.5 \u03bcm<\/li><li>63 fps @ 2048 x 2048 pixels, 11-bit data<\/li><li>QEmax> 95%<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-22 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-f250e580-0f18-4ab0-bde4-1c00ba96d0ed\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-f250e580-0f18-4ab0-bde4-1c00ba96d0ed\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-9-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Leica TCS SP8 WLL SMD-FLIM<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-9-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"210\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_flim-regular280.jpg\" alt=\"\" class=\"wp-image-362\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Inverted confocal microscope<\/li>\n\n\n\n<li>WLL and internal spectral detection unit<\/li>\n\n\n\n<li>FLIM and FCS<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Versatile inverted fluorescence confocal microscope equipped with 405 nm, 445 nm and white light laser, spectrally tunable detection and FLIM hardware. Presence of camera and epifluorescence lamp allows also widefield imaging. For more detailed microscope characteristics please see the section \u201cSpecifications\u201d.<\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Multi-color confocal imaging<\/li>\n\n\n\n<li>Multi-color widefield imaging<\/li>\n\n\n\n<li>Various measurement options: z-stack, time series, tile scan, multi positions<\/li>\n\n\n\n<li>Spectral imaging<\/li>\n\n\n\n<li>Measurement of emission and excitation spectra\u00a0<\/li>\n\n\n\n<li>Lifetime imaging, FLIM-FRET<\/li>\n\n\n\n<li>Measurement of e.g. membrane dynamics by FCS and FRAP<\/li>\n\n\n\n<li>Live-cell imaging available: stage-top incubator with controlled humidity and atmosphere (CO<sub>2<\/sub>+N<sub>2<\/sub>)\u00a0<\/li>\n\n\n\n<li>Brightfield, DIC, polarized light microscopy<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>An inverted confocal microscope Dmi8 with a laser scanning confocal head Leica TCS SP8, a motorized microscope stage with a Super Z-galvo scanning insert for fast and precise Z movement, HW autofocus and Best focus control, transmitted light LED U-12V, square pinhole of tunable size and following units:<\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-213e6a66-5ea6-40c0-bdce-b8d535585b87\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-213e6a66-5ea6-40c0-bdce-b8d535585b87\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-213e6a66-5ea6-40c0-bdce-b8d535585b87\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-213e6a66-5ea6-40c0-bdce-b8d535585b87\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-213e6a66-5ea6-40c0-bdce-b8d535585b87\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-33\" class=\"tablepress tablepress-id-33\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">LAS-X<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Epifluorescence <\/h5><\/td><td class=\"column-2\">CoolLED pE-300<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Lasers<\/h5><\/td><td class=\"column-2\"><ul><li><b>405 nm<\/b>(max 40 MHz, 3 mW)<\/li><li><b>WLL 470-670 nm <\/b> (max 80 MHz, 1.5 mW)<\/li><ul><li type=\"circle\">laser wavelength and intensity is controlled by a combination of AOTF and AOBS<\/li type=\"circle\"><li type=\"circle\"> the actual wavelength selectable with 1 nm step<\/li type=\"circle\"><li type=\"circle\">up to 8 parallel laser lines<\/li type=\"circle\"><\/ul><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Additional beamsplitters<\/h5><\/td><td class=\"column-2\">BP 440\/40<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Filter turret (filter cubes for epifluorescence lamp)<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-34\" class=\"tablepress tablepress-id-34\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\">DAPI <\/td><td class=\"column-2\">EX 325-375, BS 400, EM 435-485<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\">FITC<\/td><td class=\"column-2\">EX 460-500, BS 505, EM 512-542<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">RHOD<\/td><td class=\"column-2\">EX 541-551, BS 560, EM 565-605<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">CY5<\/td><td class=\"column-2\">EX 590-650, BS 660, EM 662-738<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Analyser<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-36\" class=\"tablepress tablepress-id-36\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Objective Type<\/th><th class=\"column-2\">Magnification<\/th><th class=\"column-3\">Immersion<\/th><th class=\"column-4\">N.A.<\/th><th class=\"column-5\">WD[mm]<\/th><th class=\"column-6\">DIC\/Correct. Ring<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">HC PL FLUOTAR 1.6x<\/td><td class=\"column-2\">1.6x<\/td><td class=\"column-3\">Dry<\/td><td class=\"column-4\">0.05<\/td><td class=\"column-5\">1.54<\/td><td class=\"column-6\">-<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">HC PL APO CS 10x<\/td><td class=\"column-2\">10x<\/td><td class=\"column-3\">Dry<\/td><td class=\"column-4\">0.40<\/td><td class=\"column-5\">2.20<\/td><td class=\"column-6\">-<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">HC PL APO CS2 20x<\/td><td class=\"column-2\">20x<\/td><td class=\"column-3\">Dry<\/td><td class=\"column-4\">0.75<\/td><td class=\"column-5\">0.62<\/td><td class=\"column-6\">DIC<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">HC PL APO CS2 63x WI<\/td><td class=\"column-2\">63x<\/td><td class=\"column-3\">Water<\/td><td class=\"column-4\">1.20<\/td><td class=\"column-5\">0.30<\/td><td class=\"column-6\">DIC, motorized correction collar<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">HC PL APO CS2 63x Oil<\/td><td class=\"column-2\">63x<\/td><td class=\"column-3\">Oil<\/td><td class=\"column-4\">1.40<\/td><td class=\"column-5\">0.14<\/td><td class=\"column-6\">DIC<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Scanner<\/h5><\/td><td class=\"column-2\">FOV SP8 scanner<ul><li> maximal sampling 8192x8192px<\/li><li>speed 1-1800 Hz (7fps@512x512 px)<\/li><li>hardware zoom 0.75x-48x<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Fluorifier Disc Settings (notch filters)<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-37\" class=\"tablepress tablepress-id-37\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Turret 1<\/th><th class=\"column-2\">Turret 2<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">NF 488<\/td><td class=\"column-2\">NF 445<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">NF 514<\/td><td class=\"column-2\">NF 594<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">NF 458\/514<\/td><td class=\"column-2\">NF 445\/594<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">NF 488\/561\/633<\/td><td class=\"column-2\">SMD1 NF 405\/470<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">MBS 488\/561<\/td><td class=\"column-2\">SMD2 NF 405\/640<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\">MBS 488\/561\/633<\/td><td class=\"column-2\">SMD3 NF 470\/640<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><h5>Detection<\/h5><\/td><td class=\"column-2\"><b>Transmitted light detector<\/b> (PMT)<br \/>\n<b>Internal spectrally tunable detection unit<\/b><br \/>\n<ul><li><b>3x HyD detectors<\/b> for FLIM (time-gating possible), GaAsP, 400 \u2013 720 nm, modes: Standard, Counting, BrightR; QE=45%@500nm<\/li><li><b>2x PMT detectors,<\/b>400-800nm, QE=30%@500nm<\/li><\/ul><br \/>\n<b>Camera DFC365 FX<\/b> (Leica)<br \/>\n<ul><li>1392 x 1040 pixels<\/li><li>6.45 \u03bcm x 6.45 \u03bcm<\/li><li>CCD sensor Sony ICX285<\/li><li>C-mount 0.7x<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><h5>FLIM and FCS module<\/h5><\/td><td class=\"column-2\"><b>HydraHarp400<\/b> (Picoquant)<ul><li>Picosecond Event Timer &amp; TCSPC Module<\/li><li>Four channels available<\/li><li>Enables detection of photon arrival times with respect to the beginning of the experiment, the excitation pulse and the channel in which the photon was registered (TTTR mode). In combination with a scanner controller, a histogram acquisition at different image positions is available.<\/li><\/ul><b>Symphotime64<\/b><br \/>\n<ul><li>Software for image acquisition and analysis<\/li><\/ul>Custom written software in LabView for image acquisition and data analysis.<\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\"><h5>Navigator module<\/h5><\/td><td class=\"column-2\"><b>LAS-X Navigator<\/b> - software module for quick navigation in the sample and automated stage experiments<\/td>\n<\/tr>\n<tr class=\"row-12\">\n\t<td class=\"column-1\"><h5>Live cell imaging<\/h5><\/td><td class=\"column-2\"><b>OKOlab incubation system<\/b> - regulation of temperature and CO2 and N2 level<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-33 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-213e6a66-5ea6-40c0-bdce-b8d535585b87\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-213e6a66-5ea6-40c0-bdce-b8d535585b87\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-10-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Leica TCS SP8 DM6 CFS<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-10-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"373\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_tcs_1-regular280.jpg\" alt=\"\" class=\"wp-image-361\" srcset=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_tcs_1-regular280.jpg 280w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_tcs_1-regular280-225x300.jpg 225w\" sizes=\"auto, (max-width: 280px) 100vw, 280px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Upright confocal microscope<\/li>\n\n\n\n<li>Internal spectral detection unit<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>An upright fluorescence confocal microscope equipped with four excitation lasers, dipping objectives with long working distance, oil immersion objective and 1x HyD detector and 2x PMTs. Presence of camera, epifluorescence lamp and removable infrared filter allows also widefield imaging in infrared area. Accessory for electrophysiological experiments is also available. For more detailed microscope characteristics please see the section \u201cSpecifications\u201d.<a href=\"\"><\/a><\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Fast and multi-color confocal imaging<\/li>\n\n\n\n<li>Fast and multi-color widefield imaging\u00a0<\/li>\n\n\n\n<li>Various measurement options: z-stack, time series<\/li>\n\n\n\n<li>Spectral imaging, measurement of emission spectrum<\/li>\n\n\n\n<li>Option of infrared detection in widefield imaging\u00a0\u00a0<\/li>\n\n\n\n<li>Brightfield and polarized light microscopy<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>An upright confocal microscope DM6 CFS with a laser scanning confocal head Leica TCS SP8, a manual microscope stage, Best focus control, transmitted light LED U-12V, removable infrared filter, square pinhole of tunable size and following units:<\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-9b354778-430c-4299-ae58-f63aa75dba26\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-5a07f115-5cfa-40af-a48f-d54bb049ca16\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-5a07f115-5cfa-40af-a48f-d54bb049ca16\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-5a07f115-5cfa-40af-a48f-d54bb049ca16\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-5a07f115-5cfa-40af-a48f-d54bb049ca16\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-27\" class=\"tablepress tablepress-id-27\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">LAS-X<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Epifluorescence lamp<\/h5><\/td><td class=\"column-2\">Leica EL6000<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Lasers<\/h5><\/td><td class=\"column-2\"><li><b>445 nm<\/b> (max 40 MHz)<\/li><li><b>488 nm<\/b> (max 40 MHz)<\/li><li><b>405, 488, 552, 638 nm<\/b> (all cw)<\/li><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Beamsplitters<\/h5><\/td><td class=\"column-2\">DD 488\/552<br \/>\nTD 488\/552\/638<br \/>\nRT 15\/85<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Filter turret <br \/>\n\u2013 filter cubes for epifluorescence lamp<br \/>\n<\/h5><\/td><td class=\"column-2\"><b>DAPI<\/b>&nbsp;&nbsp;&nbsp;&nbsp;(Ex: 325-375;   DM: 400;   Em: 435-485)<br \/>\n<b>FITC<\/b>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(Ex: 460-500;   DM: 505;   Em: 512-542)<br \/>\n<b>RHOD<\/b>&nbsp;(Ex: 541-551;   DM: 560;   Em: 565-605)<br \/>\n<b>CY5<\/b>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(Ex: 590-650;   DM: 660;   Em: 662-738) \u2013 not inserted<br \/>\nAnalyser<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\"><ul><li>HC APO L 10x (W), NA 0.30, WD 3.7 mm, dipping objective<\/li><li>HC FLUOTAR L 25x (W), NA 0.95, WD 2.5 mm, dipping objective<\/li><li>HC PL APO CS2 63x Oil, NA 1.40, WD 0.14 mm<\/li><\/ul><br \/>\nTwo objectives are installed at maximum.<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Scanner<\/h5><\/td><td class=\"column-2\">FOV SP8 scanner<ul><li>maximal sampling 8192 x 8192 px<\/li><li>speed 1-1800 Hz (7fps@512 x 512 px)<\/li><li>hardware zoom 0.75x-48x<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Detection<\/h5><\/td><td class=\"column-2\"><b>Transmitted light detector<\/b> (PMT)<br \/>\n<b>Internal spectrally tunable detection unit<\/b><ul><li><b>1x HyD detectors<\/b> (GaAsP, 400 \u2013 720 nm, Modes: Standard, Counting, BrightR), QE=45%@500nm<\/li><li><b>2x  PMT detectors<\/b> (400-800nm), QE=30%@500nm<\/li><\/ul><b>Camera DFC365 FX (Leica)<\/b><ul><li>1392 x 1040 pixels<\/li><li>6.45 \u03bcm x 6.45 \u03bcm<\/li><li>CCD sensor Sony ICX285<\/li><li>C-mount 0.7x<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-27 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-5a07f115-5cfa-40af-a48f-d54bb049ca16\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-5a07f115-5cfa-40af-a48f-d54bb049ca16\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div id=\"lwf\" class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-11-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Leica DMi8 WF<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-11-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\" id=\"obrl\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"210\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/leica_dmi8-regular280.jpg\" alt=\"\" class=\"wp-image-360\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Inverted widefield microscope<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Inverted widefield fluorescence microscope equipped with epifluorescence lamp and EM-CCD camera.<a href=\"\"><\/a><\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Fast and multi-color widefield imaging<\/li>\n\n\n\n<li>Various measurement options: z-stack, time series, tile scan, multi positions<\/li>\n\n\n\n<li>Brightfield and DIC microscopy<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>An inverted widefield microscope Dmi8 with motorized microscope stage, transmitted light LED U-12V, and following units:<\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-e540ca4c-7513-4b8e-974f-be6101e16374\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-63bfc1ac-e063-4881-b525-5e01a50514e2\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-63bfc1ac-e063-4881-b525-5e01a50514e2\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-63bfc1ac-e063-4881-b525-5e01a50514e2\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-63bfc1ac-e063-4881-b525-5e01a50514e2\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-26\" class=\"tablepress tablepress-id-26\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">LAS-X, Navigator<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Epifluorescence lamp<\/h5><\/td><td class=\"column-2\">CoolLED pE-300 <\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Filter turret<\/h5><\/td><td class=\"column-2\"><b>DAPI<\/b>&nbsp;&nbsp;&nbsp;&nbsp;(Ex: 325-375;   DM: 400;   Em: 435-485)<br \/>\n<b>FITC<\/b>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(Ex: 460-500;   DM: 505;   Em: 512-542)<br \/>\n<b>RHOD<\/b>&nbsp;(Ex: 541-551;   DM: 560;   Em: 565-605)<br \/>\n<b>CY5<\/b>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(Ex: 590-650;   DM: 660;   Em: 662-738)<br \/>\nAnalyser<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\"><ul><li>HC PL FLUOTAR 5x, NA 0.15, WD 13.7 mm<\/li><li>HC PL FLUOTAR 10x, NA 0.30, WD 11 mm<\/li><li>HC PL FLUOTAR L 20x, NA 0.40, WD 6.9 mm<\/li><li>HC PL FLUOTAR L 40x, NA 0.60, WD 3.3 mm<\/li><li>HC PL APO CS2 63x WI, NA 1.20, WD 0.30 mm, DIC<\/li><li>HC PL APO 63x Oil, NA 1.4, WD 0.14 mm, DIC<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Camera: left port<\/h5><\/td><td class=\"column-2\"><b><a href=\"https:\/\/www.hamamatsu.com\/us\/en\/product\/cameras\/cmos-cameras\/C13440-20CU.html\"> ORCA-Flash4.0 V3<\/a><\/b> (Hamamatsu)<ul><li>monochrome digital sCMOS camera<\/li><li>2048 x 2048 pixels<\/li><li>pixel size: 6.5x6.5 \u03bcm<\/li><li>readout rate 40 fps for 16bit full frame<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Camera: right port<\/h5><\/td><td class=\"column-2\"><b>K3C<\/b> (Leica)<ul><li> color digital CMOS camera<\/li><li>3072 x 2048 pixels<\/li><li>pixel size: 2.4 x 2.4 \u03bcm<\/li><li>bit depth: 3x12bit RGB<\/li><li>readout rate 15 fps<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-26 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-63bfc1ac-e063-4881-b525-5e01a50514e2\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-63bfc1ac-e063-4881-b525-5e01a50514e2\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-12-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Carl Zeiss Axiozoom.V16<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-12-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"210\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2020\/06\/carl_zeiss-regular280.jpg\" alt=\"\" class=\"wp-image-358\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>Upright macroscope<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Fluorescence zoom upright microscope which combines the benefits of stereomicroscopes such as zoom optics and long working distances with the higher resolutions of traditional light microscopes. The macroscope in IMCF at BIOCEV is equipped with a 2.3x magnification objective combined with numerical aperture 0.57 what offers superior brightness in large objects fields. In comparable image fields, the Axio Zoom.V16 offers a 2.5 times higher resolution and 10 times brighter fluorescence than generally used stereomicroscopes. See the <a href=\"https:\/\/www.zeiss.com\/microscopy\/en\/products\/light-microscopes\/stereo-and-zoom-microscopes\/axio-zoom-v16-for-biology.html\" target=\"_blank\" rel=\"noopener\">product web page<\/a>.<\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>visualization of large samples, e.g. whole model organism, in fluorescence contrast or transmitted light with the possibility to zoom in to the smallest details of the sample. Long working distance of the available objectives allows comfortable manipulation with specimens.\u00a0<\/li>\n\n\n\n<li>basic documentation of histological samples.<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>Axiozoom.V16 is a fluorescence zoom upright microscope equipped with a motorized stage with travel range 150 x 100 mm and the following<strong>:<\/strong><\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-2f70c537-4fbe-423c-903e-2a3269308dc0\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-2f70c537-4fbe-423c-903e-2a3269308dc0\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-2f70c537-4fbe-423c-903e-2a3269308dc0\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-2f70c537-4fbe-423c-903e-2a3269308dc0\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-2f70c537-4fbe-423c-903e-2a3269308dc0\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-25\" class=\"tablepress tablepress-id-25\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">ZEN blue pro 2012<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Illumination<\/h5><\/td><td class=\"column-2\"><ul><li>Transmitted light \u2013 Zeiss CL 9000 LED CAN with day-light filter for cold light LED<\/li><li>Fluorescence - HXP 120 V (D) \u2013 long-life, metal halide illuminator<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Filter cubes<\/h5><\/td><td class=\"column-2\"><b> Filter set 49 DAPI<\/b>&nbsp;&nbsp;&nbsp;&nbsp;(Ex: 365;   DM: 395;   Em: 445\/450)<br \/>\n<b>Filter set 38 GFP<\/b>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(Ex: 470\/40;   DM: 495;   Em: 525\/550)<br \/>\n<b>Filter set 63 RFP<\/b>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(Ex: 572\/25;   DM: 590;   Em: 629\/62)<br \/>\n<b>Filter set 50 CY5<\/b>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(Ex: 640\/30;   DM: 660;   Em: 690\/50)<br \/>\n<br \/>\nAll filter sets are exchangeable.<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\">\n<table id=\"tablepress-29\" class=\"tablepress tablepress-id-29\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Objective Type<\/th><th class=\"column-2\">Immersion<\/th><th class=\"column-3\">Magnification<\/th><th class=\"column-4\">N.A.<\/th><th class=\"column-5\">WD[mm]<\/th><th class=\"column-6\">DIC\/Correct. Ring<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">PlanNeoFluar Z<\/td><td class=\"column-2\">Air<\/td><td class=\"column-3\">1x<\/td><td class=\"column-4\">0.25<\/td><td class=\"column-5\">56<\/td><td class=\"column-6\">-<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">PlanNeoFluar Z<\/td><td class=\"column-2\">Air<\/td><td class=\"column-3\">2.3x<\/td><td class=\"column-4\">0.57<\/td><td class=\"column-5\">10.6<\/td><td class=\"column-6\">-<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Detection<\/h5><\/td><td class=\"column-2\">Camera Axiocam 506 mono<ul><li> 6 Mpix (2752x2208)<\/li><li> pixel size 4,54 \u03bcm x 4.54 \u03bcm<\/li><li>19 fps at full frame<\/li><li>effective sensor size 12.5 x 10.0 mm<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-25 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-2f70c537-4fbe-423c-903e-2a3269308dc0\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-2f70c537-4fbe-423c-903e-2a3269308dc0\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-13-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Akoya PhenoCycler-Fusion 2.0<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-13-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"998\" height=\"614\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/08\/Akoya-Phenocycler-2.jpg\" alt=\"\" class=\"wp-image-2612\" srcset=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/08\/Akoya-Phenocycler-2.jpg 998w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/08\/Akoya-Phenocycler-2-300x185.jpg 300w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/08\/Akoya-Phenocycler-2-768x472.jpg 768w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2024\/08\/Akoya-Phenocycler-2-488x300.jpg 488w\" sizes=\"auto, (max-width: 998px) 100vw, 998px\" \/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p>PhenoCycler-Fusion 2.0 enables to detect protein expression on tissues in real time. The system introduces up to 64 cycles automation with rapid fluidics establishing itself as the fastest spatial biology solution enabling to detect up to 100+ biomarkers (3 markers per run plus DAPI as reference). It is a part of an integrated end-to-end workflow containing pre-optimized antibodies and reagents, fast fluidics and imaging, as well as onboard data processing generating high-resolution QPTIFF files. The system features imaging of 1 million cells in 10 minutes by the detection of biomarkers while preserving tissue integrity using barcoding technology. The system allows to stain the tissue also with non-inventoried antibodies conjugated with barcodes via kit. Panel could be built up from an extensive database of antibodies, in-house conjugated antibodies or Akoya offers ready to-use PhenoCode\u2122 Discovery Panels<\/p>\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>For FFPE and FF tissue sections, tissue microchips, tissue sections, organoids<\/li>\n\n\n\n<li>Up to 100+ biomarkers on one section by unique barcoding technology<\/li>\n\n\n\n<li>Up to 300+ catalog antibodies or in-house conjugated antibodies<\/li>\n\n\n\n<li>Cell phenotyping<\/li>\n\n\n\n<li>Cellular neighborhood analysis<\/li>\n\n\n\n<li>Slide scanning without multiplexing, brightfield, and fluorescence<\/li>\n\n\n\n<li>25 min of acquisition for 4 colors, at 15 mm x 15 mm, at 20x<\/li>\n\n\n\n<li>QPTIFF file format compatible with Enable Medicine, Visiopharm, QuPath etc.<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-2c590546-01ad-4d7d-b167-ea26e37d9046\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-c71a1340-90bb-4dd9-ae4c-0a98b7e34e5a\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-c71a1340-90bb-4dd9-ae4c-0a98b7e34e5a\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-c71a1340-90bb-4dd9-ae4c-0a98b7e34e5a\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-c71a1340-90bb-4dd9-ae4c-0a98b7e34e5a\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-74\" class=\"tablepress tablepress-id-74\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Channels<\/h5><\/td><td class=\"column-2\">Opal 7-color<br \/>\nDAPI<br \/>\nAF488<br \/>\nATTO 550<br \/>\nCy5<br \/>\nAF750<br \/>\nBrightfield<br \/>\n<br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Magnification<\/h5><\/td><td class=\"column-2\">10x (NA 0.4)<br \/>\n20x (NA 0.7)<br \/>\n40x (NA 0.8)<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Resolution<\/h5><\/td><td class=\"column-2\">10x (1.0 \u00b5m\/pixel)<br \/>\n20x (0.5 \u00b5m\/pixel)<br \/>\n40x (0.25 \u00b5m\/pixel)<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Scanning area<\/h5><\/td><td class=\"column-2\">18mm x 35mm<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Number of slides processed simultaneously<\/h5><\/td><td class=\"column-2\">2 (multiple tissue sections per slide)<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">PhenoCycler-Fusion 2.3.1.<br \/>\nPhenoCycler Experiment Designer 2.1.0.<br \/>\nPhenochart 2.2.0.<br \/>\nQuPath<br \/>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-74 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-c71a1340-90bb-4dd9-ae4c-0a98b7e34e5a\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-c71a1340-90bb-4dd9-ae4c-0a98b7e34e5a\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-14-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>Nanolive CX-A<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-14-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"280\" height=\"210\" src=\"http:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2022\/11\/Nanolive.png\" alt=\"\" class=\"wp-image-1498\"><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li>3D holotomography &amp; fluorescence<\/li>\n\n\n\n<li>Stage-top incubator for long-term automatic live cell imaging<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Automated holotomographic and fluorescence microscope for long-term live-cell imaging. Label-free 3D imaging of cells in real time is ensured due to the low power laser and top stage. In addition, presence of epifluorescence lamp allows correlative holographic and fluorescence imaging. Link to manufacturer website:<\/p>\n\n\n\n<p>https:\/\/www.nanolive.ch\/products\/3d-microscopes\/cx-a\/<a href=\"\"><\/a><\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Gentle long-term live-cell label-free 3D imaging<\/li>\n\n\n\n<li>Correlative holographic (3D) and fluorescence (2D) imaging<\/li>\n\n\n\n<li>Imaging of cell populations, single cells or organelles<\/li>\n\n\n\n<li>Measurement of refractive indices inside the cell<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-e10d0edf-6edb-4dad-8043-0202085c9590\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-e10d0edf-6edb-4dad-8043-0202085c9590\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-e10d0edf-6edb-4dad-8043-0202085c9590\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-e10d0edf-6edb-4dad-8043-0202085c9590\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-e10d0edf-6edb-4dad-8043-0202085c9590\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-23\" class=\"tablepress tablepress-id-23\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">EVE - data acquisition\t<br \/>\nSTEVE - data visualization and post-processing<\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Illumination<\/h5><\/td><td class=\"column-2\">520 nm (Class 1 laser low power, sample exposure 0.2 mW\/mm2)<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Epifluorescence lamp<\/h5><\/td><td class=\"column-2\">CoolLED pE-300<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Excitation filters<\/h5><\/td><td class=\"column-2\">392\/23, 474\/27, 554\/23 or 635\/18<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Emission filters<\/h5><\/td><td class=\"column-2\">For DAPI, FITC, TRITC\/Cy5 imaging (Semrock FF01-432\/515\/595\/730) <\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Objective<\/h5><\/td><td class=\"column-2\">Dry objective 60x, NA 0,8<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\"><h5>Camera<\/h5><\/td><td class=\"column-2\"><b>USB 3.0 CMOS Sony IMX174 sensor<\/b> <br \/>\n<ul><li>Quantum Efficiency 70 % (at 545 nm)<\/li><li>Dark Noise 6,6 e\u00af <\/li><li>Dynamic Range 73,7 dB<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\"><h5>Module for environmental control<\/h5><\/td><td class=\"column-2\"><b>Tokaihit stage top incubator<\/b><br \/>\n<ul><li>CO2 concentration range: 5% - 20% (\u00b10.1%)<\/li><li>Humidity: ~ 95%<\/li><li>Sample temperature: 30-40\u00b0C (\u00b10.3\u00b0C)<\/li><\/ul><br \/>\n<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\"><h5>Sample insert<\/h5><\/td><td class=\"column-2\">4 dish format<br \/>\n96 well plate<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\"><h5>Recommended labware<\/h5><\/td><td class=\"column-2\"><b>35 mm low IBIDI dish<\/b> Cat.No: 80136; optional DIC lid Cat.No: 80050; recommendations: respect dish volume (1-2 ml), use same volume in each well, assure no meniscus, assure no bubbles, change medium before imaging)<br \/>\n<b>Nanolive 96-well format plates<\/b> \u2013 recommendations: respect well volume (80-90\u03bcl), each well must have the same volume<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-23 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-e10d0edf-6edb-4dad-8043-0202085c9590\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-e10d0edf-6edb-4dad-8043-0202085c9590\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n\n<div class=\"wp-block-ub-content-toggle-accordion\" style=\"border-color: #f1f1f1; \" id=\"ub-content-toggle-panel-block-71dcd60e-7279-4a5d-a344-01fe0548eddd\">\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-title-wrap\" style=\"background-color: #f1f1f1;\" aria-controls=\"ub-content-toggle-panel-15-c7689ffa-8ae7-4152-b389-68571d10b70d\" tabindex=\"0\">\n\t\t\t<p class=\"wp-block-ub-content-toggle-accordion-title ub-content-toggle-title-c7689ffa-8ae7-4152-b389-68571d10b70d\" style=\"color: #000000; \"><strong>ImageXpress Micro XLS<\/strong><\/p>\n\t\t\t<div class=\"wp-block-ub-content-toggle-accordion-toggle-wrap right\" style=\"color: #000000;\"><span class=\"wp-block-ub-content-toggle-accordion-state-indicator wp-block-ub-chevron-down\"><\/span><\/div>\n\t\t<\/div>\n\t\t\t<div role=\"region\" aria-expanded=\"false\" class=\"wp-block-ub-content-toggle-accordion-content-wrap ub-hide\" id=\"ub-content-toggle-panel-15-c7689ffa-8ae7-4152-b389-68571d10b70d\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.3%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"2330\" height=\"1312\" src=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2023\/09\/20230913_105545.jpg\" alt=\"\" class=\"wp-image-2302\" style=\"object-fit:cover\" srcset=\"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2023\/09\/20230913_105545.jpg 2330w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2023\/09\/20230913_105545-300x169.jpg 300w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2023\/09\/20230913_105545-1024x577.jpg 1024w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2023\/09\/20230913_105545-768x432.jpg 768w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2023\/09\/20230913_105545-1536x865.jpg 1536w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2023\/09\/20230913_105545-2048x1153.jpg 2048w, https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-content\/uploads\/2023\/09\/20230913_105545-500x282.jpg 500w\" sizes=\"auto, (max-width: 2330px) 100vw, 2330px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.7%\">\n<ul class=\"wp-block-list\">\n<li>cytometry &amp; fluorescence<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<p>Fully automated imaging cytometer designed for fast acquisition and analysis of robust data volume by advanced methods of multiparametric cytometric analysis, reconstruction and multidimensional visualization. Link to manufacturer website:<\/p>\n\n\n\n<p><a href=\"https:\/\/www.moleculardevices.com\/systems\/high-content-imaging\/imagexpress-micro-xls-widefield-high-content-analysis-system\">https:\/\/www.moleculardevices.com\/systems\/high-content-imaging\/imagexpress-micro-xls-widefield-high-content-analysis-system<\/a><a href=\"\"><\/a><\/p>\n\n\n\n<p><strong>Applications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>localization, quantification and functional analysis of mitochondria in cell, effectivity and dynamics of internalization of surface structures, viability and morphology of cells or subcellular structures<\/li>\n\n\n\n<li>cytotoxicity, apoptosis\/necrosis<\/li>\n\n\n\n<li>cell cycle and mitosis<\/li>\n\n\n\n<li>cell differentiation, number, viability and phosphorylation of cells or subcellular structures, activation\/inhibition of signal pathways and phosphorylation of proteins, effectivity of transfection, cell migration and analysis of objects movement<\/li>\n\n\n\n<li>effectivity and dynamics of internalization of surface structures, localization and co-localization of proteins in nuclear, perinuclear, cytoplasmic or membrane localization<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>Automatic hardware autofocus via IR laser. Automatic software autofocus.<\/p>\n\n\n<div class=\"wp-block-ub-expand ub-expand\" id=\"ub-expand-dc692596-ed51-44b1-b2d8-c6f9b64b3faa\" data-scroll-type=\"false\" data-scroll-amount=\"\" data-scroll-target=\"\">\n<div class=\"ub-expand-portion ub-expand-partial wp-block-ub-expand-portion\" id=\"ub-expand-partial-dc692596-ed51-44b1-b2d8-c6f9b64b3faa\" aria-hidden=\"false\">\n\t\t\t\n\t\t\t<a id=\"ub-expand-toggle-partial-dc692596-ed51-44b1-b2d8-c6f9b64b3faa\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"false\" aria-controls=\"ub-expand-full-dc692596-ed51-44b1-b2d8-c6f9b64b3faa\" tabindex=\"0\">\n\t\t\t\tshow more\n\t\t\t<\/a>\n\t\t<\/div>\n\n<div class=\"ub-expand-portion ub-expand-full ub-hide wp-block-ub-expand-portion\" id=\"ub-expand-full-dc692596-ed51-44b1-b2d8-c6f9b64b3faa\" aria-hidden=\"true\">\n\t\t\t\n\n<table id=\"tablepress-69\" class=\"tablepress tablepress-id-69\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><h5>Software<\/h5><\/td><td class=\"column-2\">1 offline licence<br \/>\nModules:<ul><li>Granularity Application Module<\/li> <li>Live\/Dead Application Module <\/li><li>Cell cycle Application Module <\/li><li>Multiwavelenght Cell Scoring Application Module <\/li><li>Multiwavelenght Translocation Application Module <\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><h5>Epifluorecence<\/h5><\/td><td class=\"column-2\">solid state with spectral range 400-640nm and 10 000 hours life time<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><h5>Objectives<\/h5><\/td><td class=\"column-2\"><ul><li>10x Plan Fluor, NA 0.3 <\/li><li>20x Ph1 S Plan Fluor ELWD ADM, NA 0.45, appropriate for phase contrast<\/li><li>40x Ph2 S Plan Fluor ELWD ADM, NA 0.60, appropriate for phase contrast<\/li><\/ul><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\"><h5>Filters <\/h5><\/td><td class=\"column-2\">DAPI<br \/>\neGFP\/FITC<br \/>\nTRITC<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\"><h5>Detector<\/h5><\/td><td class=\"column-2\">SCMOS<br \/>\n4.5 MPx, 80-200 frames per sec., 16bit A\/D converter, dynamic range 1:20000, low electronic noise 1.4 e\/px<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\"><h5>Stage<\/h5><\/td><td class=\"column-2\">inserts for multiwall plates (6 well \u2013 1536 well)<br \/>\nmicroscopic slides (possible up to 3 slides simultaneously)<br \/>\nadjustable in X, Y, Z with 100nm accuracy<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-69 from cache -->\n\n\n\t\t\t<a id=\"ub-expand-toggle-full-dc692596-ed51-44b1-b2d8-c6f9b64b3faa\" class=\"ub-expand-toggle-button\" style=\"text-align: left; \" role=\"button\" aria-expanded=\"true\" aria-controls=\"ub-expand-full-dc692596-ed51-44b1-b2d8-c6f9b64b3faa\" tabindex=\"0\">\n\t\t\t\tshow less\n\t\t\t<\/a>\n\t\t<\/div>\n<\/div>\n<\/div>\n\t\t<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"sidebar-page.php","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-1353","page","type-page","status-publish","hentry"],"featured_image_src":null,"_links":{"self":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/1353","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/comments?post=1353"}],"version-history":[{"count":98,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/1353\/revisions"}],"predecessor-version":[{"id":3000,"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/pages\/1353\/revisions\/3000"}],"wp:attachment":[{"href":"https:\/\/imcf.natur.cuni.cz\/IMCF\/wp-json\/wp\/v2\/media?parent=1353"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}