Practical Course

SIS code: MB100P05

Practical hands-on training on 3D correlative light and electron microscopy combining confocal fluorescence imaging and electron microscopy imaging contain focused ion beam milling (FIB-SEM) and electron tomography (ET).

November 22 - 25, 2021

BIOCEV, Vestec

Light, particulary fluorescence, microscopy offers live-cell compatibility and flexibility in labelling by specific molecular probes. In this way, insight into the function of cells at their native state can be obtained. Electron microscopy, on the hand, provides unmatched spatial resolution. Correlative light and electron microscopy (CLEM) combines the unique information from both techniques in a single image.During the course you will try hands-on a complete CLEM workflow from sample preparation to image processing. The hands-on sessions will be conducted using the state-of-the-art equipment available at IMCF, including high end fluorescence confocal microscopes, scanning electron microscope (SEM) focused ion beam (FIB) milling and transmission electron microscope (TEM).

Registration deadline: November 5, 2021

The course is supported by the National Infrastructure for Biological and Medical Imaging (Czech-BioImaging, Ministry of Education, Youth and Sports – Large Research Infrastructure, LM2018129).