Practical Course

Hands-on training of selected electron microscopy techniques. The course consists of three parts. The first comprises preparation of biological samples through a standard, chemical fixation procedure. The second part continues with the ultramicrotomy of different resin-embedded specimens. The third part deals with the immunogold labelling on resin sections.

The list of hands-on techniques covered by EM-SPS course:

Chemical fixation

• Chemical fixation of suspension and monolayer cells
• Different embedding procedures

Ultramicrotomy

• Semithin sectioning and toluidine blue section staining
• Ultrathin sectioning and section pick up techniques
• Sections post-contrasting
• Troubleshooting and tips-and-tricks
• TEM imaging of different sample types

Immunolabelling

• On section immunolabelling with primary and gold-coupled antibodies

EM-SPS workshop

BIOCEV, Vestec

The course is organized by the Imaging Methods Core Facility as annual course.

The EM-SPS course has been postponed till autumn 2022.

The course is supported by the National Infrastructure for Biological and Medical Imaging (Czech-BioImaging, Ministry of Education, Youth and Sports – Large Research Infrastructure, LM2018129).